Analysis of protein additives degradation in aged mortars using mass spectrometry and principal component analysis

Kučková, Štěpánka; Rambouskova, Gabriela; Junková, Petra; Šantrůček, Jiří; Cejnar, Pavel; Smirnova, Tatiana Anatolievna; Novotný, Ondřej; Hynek, Radovan

doi:10.1016/j.conbuildmat.2021.123124

Cited by 10 publications

(2 citation statements)

References 44 publications

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“…Spectra were collected by the flexControl software (Bruker Daltonics) in positive reflector mode in the mass range of 900−4500 m/z [25]. The resulting spectra were processed with mMass [26]. For PCA, MS‐alone and multiMS‐toolbox [24, 27] were used with default values and normalization by total ion count, and subsequently, for confirmation of the PCA results, by more normalization method [28] using linear registration in both m/z and intensity values.…”

Section: Methodsmentioning

confidence: 99%

Comparison of proteomic approaches used for the detection of potential biomarkers of Alzheimer's disease in blood plasma

Smirnova

Viskin

Hoskova

et al. 2021

J of Separation Science

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At present, Alzheimer's disease is detected mainly using psychological tests, which can only confirm the disease in its more advanced phases. Therefore, bioanalytical possibilities for detecting this disease earlier are being investigated. To date, the results of analyses, which focus mainly on the study of lipids and proteins either in cerebrospinal fluid or much less often in blood plasma, do not provide satisfactory results. In addition, cerebrospinal fluid sampling is uncomfortable for the patients and involves many health risks. In this work, we deal with proteomic analysis using Matrix‐Assisted Laser Desorption/Ionisation—Time of Flight and Liquid Chromatography coupled to tandem Mass Spectrometry of blood plasma with a focus on various ways of preanalytical sample treatments. This should lead to results improvement and facilitate the subsequent evaluation using principal component analysis and partial least squares discriminant analysis. The obtained results indicate the direction of further research, namely the study of interactions between proteins and lipids contained in blood plasma. These substances may be regarded as potential biomarkers allowing for the diagnosis of Alzheimer´s disease even in its early stages.

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Section: Methodsmentioning

confidence: 99%

Comparison of proteomic approaches used for the detection of potential biomarkers of Alzheimer's disease in blood plasma

Smirnova

Viskin

Hoskova

et al. 2021

J of Separation Science

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“…The presence of protein varies according to the type of sample. The presence of protein detected in mortar or plaster samples is usually caused by protein-based additives in the material such as eggs, blood, casein, tow, plant fibers, and animal hair [33][34][35].…”

Section: Spot Tests and Conductivity Analysismentioning

confidence: 99%

Evaluation of Material Characteristics and Structural Dynamic Properties of a Historical Church: The Case of 19th Century Bursa Derekoy Church (Ayia Paraskevi)

Ayengin,

Bağbancı,

Köprülü Bağbancı

2024

Period. Polytech. Civil Eng.

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Derekoy (Ayia Paraskevi) Church, built by the Orthodox Greeks in Bursa, is one of the prominent monuments built in the 19th century. The structure, which lost its community with the population exchange (1923) between Turkey and Greece, has since then been out of service and a rapid deterioration process has begun. Analysis works are frequently implemented for this purpose and become crucial in Turkey, of which a large part is within the earthquake zone. However, the majority of the studies on the preservation of historical buildings are lacking in detailed material analysis and numerical methods. Conducting a series of material tests and numerical analyses for the structure provides more reliable and qualified interventions since they influence the decisions on the reinforcement and protection methods employed for historical buildings. For this purpose, the material characterizations of the mortar, plaster and stones used in the church were carried out by performing physical, chemical, mechanical, petrographic and mineralogical tests on samples taken from the church. Afterwards, mechanical tests of the walls and vibration tests were conducted to determine the dynamic behavior of the building. Based on the results obtained from the analyses, a series of intervention methods were proposed. It is understood that the flat-jack, shear and vibration tests are vital for determining the real structural behavior of the building. Furthermore, it is recommended that vibration tests are carried out on both the overall structure and individual walls to determine the dynamic behavior of similar structures where only outer walls remain standing.

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Estimation of the linseed oil content in historic lime mortar

Bauerová

Kracík-Štorkánová²,

Mácová

et al. 2022

J Therm Anal Calorim

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Analysis of protein additives degradation in aged mortars using mass spectrometry and principal component analysis

Cited by 10 publications

References 44 publications

Comparison of proteomic approaches used for the detection of potential biomarkers of Alzheimer's disease in blood plasma

Comparison of proteomic approaches used for the detection of potential biomarkers of Alzheimer's disease in blood plasma

Evaluation of Material Characteristics and Structural Dynamic Properties of a Historical Church: The Case of 19th Century Bursa Derekoy Church (Ayia Paraskevi)

Estimation of the linseed oil content in historic lime mortar

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