Analysis of Potential Migrants from Plastic Materials in Milk by Liquid Chromatography–Mass Spectrometry with Liquid–Liquid Extraction and Low-Temperature Purification

Abstract

A simple and fast analytical method was developed for the determination of six UV stabilizers (Cyasorb UV-1164, Tinuvin P, Tinuvin 234, Tinuvin 326, Tinuvin 327, and Tinuvin 1577) and five antioxidants (Irgafos 168, Irganox 1010, Irganox 3114, Irganox 3790, and Irganox 565) in milk. For sample preparation liquid-liquid extraction with low-temperature purification combined with centrifugation was used to remove fats, proteins, and sugars. After the cleanup step, the sample was analyzed with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). External standard and matrix calibrations were tested. External calibration proved to be acceptable for Tinuvin P, Tinuvin 234, Tinuvin 326, Tinuvin 327, Irganox 3114, and Irganox 3790. The method was successfully validated with matrix calibration for all compounds. Method detection limits were between 0.25 and 10 μg/kg. Accuracies ranged from 93 to 109%, and intraday precisions were <13%.

Identification and Quantification of Unknown Antioxidants in Plastic Materials by Ultrasonic Extraction and Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

“…14 Prior to qualitative and quantitative analysis of antioxidants, sample pretreatment is the most important step because of the trace contents of these compounds in plastic packaging. Several pretreatment techniques have been used for extraction or purification, such as liquid-liquid extraction, [15][16][17] ultrasonic extraction, 18,19 microwave-assisted extraction, supercritical fluid extraction, solid-phase extraction, 20,21 accelerated solvent extraction, 22 solvent sublation 23 and total dissolution of the polymer. 24 However, these pretreatment and analytical methods have been focused on well-known or commonly used additives.…”

Migration of Tinuvin P and Irganox 3114 into milk and the corresponding authorised food simulant

“…The analytical method used for the determination of Tinuvin P and Irganox 3114 have already been described in detail (including validation) previously (Bodai, et al 2014),…”

Identification of Additives in Polypropylene and Their Degradation under Solar Exposure Studied by Gas Chromatography–Mass Spectrometry

“…Several works have been devoted to the evaluation of additives in polymers, − but considering the relatively high molecular weight of some additives, many of those studies use high-performance liquid chromatography (HPLC) coupled with different types of detectors, i.e., mass spectrometry (MS) or UV. The simultaneous identification of unknown complex mixtures of additives and their degradation products is not, however, straightforward by HPLC since it requires an extra effort to make the decision on the appropriate and optimal ionization mode for each different additive …”

“…For instance, they are of capital importance in polymer formulations for outdoor applications, as is the case of plastics in agriculture. In other cases, however, some additives, or their degradation products, may involve specific health safety problems, namely, in materials with applications for drinks and food packaging. ,,,− …”