Analysis of Potential Genes and Pathways Involved in the Pathogenesis of Acne by Bioinformatics

Abstract

Acne is the eighth most frequent disease worldwide. Inflammatory response runs through all stages of acne. It is complicated and is involved in innate and adaptive immunity. This study aimed to explore the candidate genes and their relative signaling pathways in inflammatory acne using data mining analysis. Microarray data GSE6475 and GSE53795, including 18 acne lesion tissues and 18 matched normal skin tissues, were obtained. Differentially expressed genes (DEGs) were filtered and subjected to functional and pathway enrichment analyses. Protein–protein interaction (PPI) network and module analyses were also performed based on the DEGs. In this work, 154 common DEGs, including 145 upregulated and 9 downregulated, were obtained from two microarray profiles. Gene Ontology and pathway enrichment of DEGs were clustered using significant enrichment analysis. A PPI network containing 110 nodes/DEGs was constructed, and 31 hub genes were obtained. Four modules in the PPI network, which mainly participated in chemokine signaling pathway, cytokine–cytokine receptor interaction, and Fc gamma R-mediated phagocytosis, were extracted. In conclusion, aberrant DEGs and pathways involved in acne pathogenesis were identified using bioinformatic analysis. The DEGs included FPR2, ITGB2, CXCL8, C3AR1, CXCL1, FCER1G, LILRB2, PTPRC, SAA1, CCR2, ICAM1, and FPR1, and the pathways included chemokine signaling pathway, cytokine–cytokine receptor interaction, and Fc gamma R-mediated phagocytosis. This study could serve as a basis for further understanding the pathogenesis and potential therapeutic targets of inflammatory acne.

Review—Current Concepts in Inflammatory Skin Diseases Evolved by Transcriptome Analysis: In-Depth Analysis of Atopic Dermatitis and Psoriasis

“…Through analyzing bacterial metabolic pathways, this approach led to a potential mechanism of supplemented VitB12-induced acne [121]. WGCNA was utilized to construct interaction networks which highlight the role of central cytokines in the disease's pathophysiology (e.g., IL1β and CXCl1/2) [122,123]. Recently, the role of miRNAs was also introduced into this research field as depicted in Table A2 [124,125].…”

Integrated Analysis of Key Genes and Pathways Involved in Nonalcoholic Steatohepatitis Improvement After Roux-en-Y Gastric Bypass Surgery

“…Evidence from both in vivo and in vitro experiments also confirmed the function of EGR1 in hepatic insulin response and hepatic lipid metabolic regulation ( 37 ). SAA1, namely, serum amyloid A-1 protein belongs to the SAA family ( 39 ). As a classical acute-phase protein produced by hepatocytes, SAA mediated infection, injury, and inflammation response and could regulate toll-like receptor 4 (TLR4), which participated in obesity-induced insulin resistance ( 40 ).…”

Elucidating the Key Biomarkers and Immune Infiltration in Acne by Integrated Bioinformatics Analysis

“…FPR1, FPR2 and FPR3 play roles in in ammatory and antibacterial processes, which are primarily expressed in leukocytes, belonged to Gi-protein-coupled receptors. C3, C3AR1, and C5AR1 belong to the core members of complement family, participate in cellular response to in ammation (44). CXCL1, CXCL8, CXCL2, CXCL3, CXCL5 are involved in guiding the neutrophils to the site of infection and promote in ammation.…”