Analysis of potential factors affecting allografts contamination at retrieval

Abstract

The microbiological contamination of retrieved tissues has become a very important topic and it is a critical aspect in the safety of allografts, especially from multi-tissue donors whose tissues are frequently contaminated as a consequence of retrieval. We analysed a total of 10,107 tissues, 8178 musculoskeletal and 1929 cardiovascular tissues, retrieved from 978 multi-tissue donors. Of these, 159 heart-beating donors (HBD) were also organ donors, while the remaining 819 non-heart-beating donors (NHBD) were tissue donors only. A multivariate logistic model was used to determine the factors affecting contamination risk during retrieval. In the model, the dependent variable was the presence/absence of contamination while the covariates included were: gender, type of donor, age of donor, cause of death, previous skin donation, cadaver time, number of people attending the retrieval, number of tissues retrieved. Moreover, a second log-linear model was used to determine the number of strains isolated per tissue. Tissue contamination was statistically correlated with gender, type of donor, cadaver time, number of people attending the retrieval and season. In conclusion, to minimize the risk of bacterial contamination, aseptic techniques should be used at retrieval, with the number of retrieval team members kept to a minimum. In addition, cadaver time should be as short as possible and the donor should be refrigerated within a few hours after death.

The Use of Microbiological Quality Control Techniques Adopted in a Human Tissue Bank

“…For cultures of bacteria or fungi that showed growth of microorganisms, the identification and quantification of colonies of microorganisms were carried out using mass spectrometry using the Microflex LT MALDI TOF equipment (Bruker, Billerica, USA) and the sensitivity test by microdilution in broth, carried out using the VITEK® 2 Compact equipment (bioMérieux, Rio de Janeiro, Brazil); sensitivity tests were performed using disk diffusion. Antimicrobial susceptibility results were interpreted in accordance with the most recent guidelines from the Brazilian Committee on Antimicrobial Susceptibility Testing/European Committee on Antimicrobial Susceptibility Testing 11,12 . The results were tabulated and presented below.…”

Experimental procedures for decontamination and microbiological testing in cardiovascular tissue banks

“…37 Paolin et al demonstrated that tissue contamination was statistically correlated with gender, type of donor, cadaver time, number of people attending the retrieval, and season. 38 These authors proposed that to minimize the risk of bacterial contamination, aseptic techniques should be used at retrieval, including restrictions on the number of team members allowed in the room. In addition, cadaver time should be as short as possible, and the donor should be refrigerated within a few hours after death.…”

“…In addition, cadaver time should be as short as possible, and the donor should be refrigerated within a few hours after death. 38 Effective tissue recovery methods such as minimizing recovery times (<24 h after death) and the number of experienced team members involved in recovery are examples of factors that can affect the rate of tissue contamination. Additional factors such as minimizing the time after asystole, the total time leading up to tissue recovery, the type of recovery site, the efficacy of the skin prep method, 34,39 and other technical procedures may also impact the rate of contamination.…”

“…In addition, cadaver time should be as short as possible, and the donor should be refrigerated within a few hours after death. 38…”

An�lise da influ�ncia de uma equipe de capta��o de pele em um Banco de Tecidos- estudo transversal

“…sentam rentabilidade maior que 2000 cm 2 por doador, por outro lado, o profissional (AW) com menor número de captações ainda apresenta uma média de 1800 cm 2 por procedimento realizado. Em recente estudo retrospectivo, os autores corroboram nossos achados e referem que a experiência da equipe de captação é fundamental para melhora da rentabilidade da captação, principalmente porque reduz a chance de contaminação do material 11 . A principal limitação deste estudo é o baixo número de captações realizadas durante o período.…”