Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis

Abstract

The objective of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the IL10, NOS2A, and ESR2 genes and chronic periodontitis (CP) and aggressive periodontitis (AgP). Three groups of patients underwent periodontal and radiographic evaluations: CP (n = 61), AgP (n = 50), and periodontally healthy (control group=61). Genomic DNA was extracted from oral epithelial cells and used for genotyping by real-time polymerase chain reaction using TaqMan® probes. The investigated SNPs were: -1087G > A, -819C > T and -592C > A in the IL10; +2087G > A in the NOS2A, and +1730G > A in the ESR2 gene. Differences in genotype and allele frequencies of each polymorphism and some individual characteristics were analyzed using the chi-square test and multivariate logistic regression analysis. Analysis of SNPs and haplotypes in the IL10 and SNP in the ESR2 gene did not present any significant association with AgP or CP. The +2087G allele of the NOS2A gene tended to be significantly associated with periodontal disease. Patients carrying the genotype +2087GG in the NOS2A gene were genetically protected against the development of CP (p = 0.05; OR = 0.44; 95%CI = 0.20-0.95). This result showed greater significance when patients with AgP and CP were combined (total PD) (p = 0.03; OR = 0.46; 95%CI = 0.23-0.92). In conclusion, the studied Brazilian population had a significantly higher frequency of the GG genotype for the +2087 SNP in the NOS2A gene in individuals without periodontitis, although statistical significance was not maintained after multiple logistic regression.

Inducible Nitric Oxide Synthase Polymorphisms and Nitric Oxide Levels in Individuals with Chronic Periodontitis

“…However, similar to other studies [36,38], this genetic association was not maintained after the adjustments for covariates in the multiple logistic regression analysis. Multiple logistic regressions were used here to evaluate the influence of common (and other potential) covariates, in the association of each NOS2 SNP with CP.…”

“…Moreover, any significant association of the +2087 SNP with CP was verified in our southeastern Brazilian population. However, in a northeastern Brazilian population, the +2087(GG) genotype was recently associated with protection against the development of PD [36]. Contrasting results for a SNP association in case-control studies considering different populations are very common, mainly because of differences in the genetic carriage (ethnicity) or in the study design [37].…”

“…Because there is only one previous study investigating the association of the above-mentioned SNPs in the NOS2 gene with CP [36], and considering that salivary NO levels in patients with CP remain controversial, we investigated in the present study the possibility of an association of susceptibility to CP with SNPs in the NOS2 gene and its haplotypes and whether these patients’ genetic carriage could be related to salivary levels of nitrite (NO 2 − ) and/or nitrate + nitrite (NOx).…”

Interleukin Expression as a Diagnostic Tool in Chronic and Aggressive Periodontitis

“…6,17 Periodontitis lesions presented a significantly higher expression of IL10 than that in autologous peripheral blood mononuclear cells. 18 Therefore, IL10 has been con sidered a candidate susceptibility gene in periodontitis.…”

“…This will allow for early treatment, thus reducing the functional and esthetic sequelae associated with the disease. 11,17 …”