Analysis of polymorphic TGFB1codons 10, 25, and 263 in a German patient group with non-syndromic cleft lip, alveolus, and palate compared with healthy adults

Scheffold, Christian; Mengsteab, Senait; Stoll, Doris; Riediger, D; Gressner, A. M.; Weiskirchen, Ralf

doi:10.1186/1471-2350-5-15

Cited by 24 publications

(19 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the contrary, Holla et al [25] could not find any significant difference in +915G/C polymorphism between CP and controls in Caucasian subjects of Czech nationality. The Table 4 Clinical characteristics of CP and G-AgP patients based on the positivity of the TGF-β1 +915C allele CP with C allele (C+) CP without C allele (C−) p G-AgP with C allele (C+) G-AgP without C allele (C−) p frequencies of +915G/C genotypes in control subjects (52.5%) of the present study were higher than the frequencies observed in other studies in healthy Caucasians [12,18,25]. Our data show that +915G/C heterozygote subjects are common in Turkish population.…”

Section: Discussioncontrasting

confidence: 83%

See 1 more Smart Citation

TGF-β1 gene polymorphisms in periodontal diseases

Atilla¹,

Emingil²,

Köse³

et al. 2006

Clinical Biochemistry

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 83%

“…The Thr263ILe polymorphism is located in exon 5 which encodes for the TGF-β1 proprotein [12]. Substitution of the polar amino acid threonine by the apolar isoleucine at codon 263 affects the stability and the activation process of TGF-β1 leading to reduced serum concentration of latent and subsequently active TGF-β1 [13,18].…”

Section: Introductionmentioning

confidence: 99%

TGF-β1 gene polymorphisms in periodontal diseases

Atilla¹,

Emingil²,

Köse³

et al. 2006

Clinical Biochemistry

View full text Add to dashboard Cite

“…The C-509T, T869C, and G915C polymorphisms have been show to be associated with the soluble TGF-b1 (sTGF-b1) serum levels [10]. Further, the homozygous G915G genotype (Arg25Arg) was associated with higher sTGF-b1 serum levels than heterozygous G915C genotype (Arg25Pro) [13,14]. The G915C TGF-b1 polymorphism has been studied in several diseases such as myocardial infarction and stroke [11], systemic lupus erytematosus [12], non-syndromic cleft lip, alveolus, and palate (CLPs) [13], juvenile idiopathic arthritis [14], ankylosing spondylitis [15], human longevity [16], and primary Sjögren's syndrome [17].…”

Section: Introductionmentioning

confidence: 98%

“…Three single-nucleotide polymorphisms within the promoter TGF-b1 gene have been identified: C-988A, G-800A, and C-509 T. In addition, a C insertion at position ?72 of the non-translated region, two polymorphisms in the signal peptide sequence at positions T869C (Leu10Pro) and G915C (Arg25Pro), and other in the precursor part of the protein C788T (Thr263Ile) have been identified [10][11][12][13]. The C-509T, T869C, and G915C polymorphisms have been show to be associated with the soluble TGF-b1 (sTGF-b1) serum levels [10].…”

Section: Introductionmentioning

confidence: 99%

The functional class evaluated in rheumatoid arthritis is associated with soluble TGF-β1 serum levels but not with G915C (Arg25Pro) TGF-β1 polymorphism

et al. 2010

View full text Add to dashboard Cite

The influence of genetic factors in rheumatoid arthritis (RA) has been described, including several cytokine genes such as transforming growth factor β (TGF-β) with regulatory effects on lymphocytes, dendritic cells, macrophages, chondrocytes, and osteoblasts, which are important in the RA pathogenesis. The G915C TGF-β1 polymorphism has been associated with soluble TGF-β1 (sTGF-β) serum levels. Thus, we studied the association of G915C (Arg25Pro) TGF-β1 polymorphism with sTGF-β1 serum levels in RA. We enrolled 120 RA patients and 120 control subjects (CS). The G915C TGF-β1 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and sTGF-β1 serum levels were quantified using an ELISA kit. The genotype frequency of G915C TGF-β1 polymorphism in RA and CS was G/G (91.7%), G/C (8.3%), C/C (0%) and G/G (85.8%), G/C (14.2%), C/C (0%), respectively, without significant differences. Moreover, the G/G TGF-β1 genotype carriers presented the highest disability index evaluated for the Spanish HAQ-DI score (P < 0.001). In addition, the sTGF-β1 serum levels were higher in RA (182.2 ng/mL) than CS (160.2 ng/mL), there was not significant difference. However, we found a positive correlation between the sTGF-β1 serum levels and the functional class (r = 0.472, P = 0.023). In conclusion, the G915C (Arg25Pro) TGF-β1 polymorphism is not associated with RA, but the sTGF-β1 serum levels are related with the functional class in RA.

show abstract

“…Bioceramics, such as hydroxyapatite (HA) and beta tri-calcium phosphate (β-TCP), possess two of the ideal characteristics for grafting procedures: osteoconduction and osseointegration [1]. Pure HA has great osteoconductive properties, ability to support the growth of bone over the implant surface, and chemical composition comparable to the inorganic component of bone [2].…”

Section: Introductionmentioning

confidence: 99%

Sintering effects on chemical and physical properties of bioactive ceramics

et al. 2013

View full text Add to dashboard Cite

Abstract:The objective of this study was to characterize the chemical and physical properties of bioactive ceramics prepared from an aqueous paste containing hydroxyapatite (HA) and beta tri-calcium phosphate (β-TCP). Prior to formulating the paste, HA and β-TCP were calcined at 800 ℃ and 975 ℃ (11 h), milled, and blended into 15%/85% HA/β-TCP volume-mixed paste. Fabricated cylindrical rods were subsequently sintered to 900 ℃, 1100 ℃ or 1250 ℃. The sintered specimens were characterized by helium pycnometry, X-ray diffraction (XRD), Fourier transform-infrared (FT-IR), and inductively coupled plasma (ICP) spectroscopy for evaluation of porosity, crystalline phase, functional-groups, and Ca:P ratio, respectively. Mechanical properties were assessed via 3-point bending and diametral compression. Qualitative microstructural evaluation using scanning electron microscopy (SEM) showed larger pores and a broader pore size distribution (PSD) for materials sintered at 900 ℃ and 1100 ℃, whereas the 1250 ℃ samples showed more uniform PSD. Porosity quantification showed significantly higher porosity for materials sintered to 900 ℃ and 1250 ℃ (p < 0.05). XRD indicated substantial deviations from the 15%/85% HA/β-TCP formulation following sintering where lower amounts of HA were observed when sintering temperature was increased. Mechanical testing demonstrated significant differences between calcination temperatures and different sintering regimes (p < 0.05). Variation in chemical composition and mechanical properties of bioactive ceramics were direct consequences of calcination and sintering.

show abstract

Analysis of polymorphic TGFB1codons 10, 25, and 263 in a German patient group with non-syndromic cleft lip, alveolus, and palate compared with healthy adults

Cited by 24 publications

References 29 publications

TGF-β1 gene polymorphisms in periodontal diseases

TGF-β1 gene polymorphisms in periodontal diseases

The functional class evaluated in rheumatoid arthritis is associated with soluble TGF-β1 serum levels but not with G915C (Arg25Pro) TGF-β1 polymorphism

Sintering effects on chemical and physical properties of bioactive ceramics

Contact Info

Product

Resources

About