Analysis of plasmid pMZ1 from<i>Micromonospora zionensis</i>

Vukov, Natǎsa; Vasiljević, Branka

doi:10.1111/j.1574-6968.1998.tb13015.x

Cited by 3 publications

References 17 publications

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Micromonospora

Genilloud¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Mi.cro.mo.no.spo'ra. Gr. adj. mikros small; Gr. adj. monos single, solitary; Gr. fem. n. spora a seed and in biology a spore; N.L. fem. n. Micromonospora small, single‐spored (organism). Actinobacteria / Actinobacteria / Micromonosporales / Micromonosporaceae / Micromonospora Well‐developed, branched, substrate mycelium (0.2–0.6 µm diameter). Nonmotile spores are borne singly, sessile, or terminally on short sporophores. Sporophore development is monopodial or in some cases sympodial. Spores are spherical to oval in shape (0.7–1.5 µm) and in most species have blunt spiny projections. The spores are often carried in branched clusters on short hyphae of the substrate mycelium. Aerial mycelium is usually absent, but some cultures develop sterile short aerial hyphae. Gram‐stain‐positive, mesophilic, non‐acid‐fast. Aerobic to microaerophilic. Chemo‐organotrophic. Sensitive to pH below 5.0. Growth usually occurs between 20 and 40°C, but not above 50°C. Cell wall contains meso ‐diaminopimelic acid and/or 3‐OH‐diaminopimelic acid. Arabinose and xylose are the characteristic sugars present in whole‐organism hydrolysates of most species, but variable amounts of galactose, glucose, mannose, and rhamnose can also be found depending on the species. The major phospholipids are phosphatidylethanolamine, phosphatidylinositol, and phosphatidylinositol mannosides. Saturated and unsaturated fatty acids are present, mostly C 15:0 iso, C 17:0 iso, C 15:0 anteiso, C 17:0 anteiso, C 17:0 10‐methyl, and C 17:0 10‐methyl. Mycolic acids are absent. Major menaquinones are MK‐9(H 4 ), MK‐9(H 6 ), MK‐10(H 4 ), MK‐10(H 6 ), or MK‐12(H 6 ). Isolated from soil, plant materials, freshwater, and marine environments. DNA G + C content ( mol %): 68–75 ( T m , HPLC). Type species : Micromonospora chalcea (Foulerton 1905) Ørskov 1923, 156 AL (“ Streptothrix chalcea ” Foulerton 1905, 1199).

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Micromonospora

Genilloud¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Vector Systems Allowing Efficient Autonomous or Integrative Gene Cloning inMicromonosporasp. Strain 40027

Zhou

Deng

2003

Appl Environ Microbiol

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Vector systems allowing autonomous or site-specific integrative gene cloning were developed for Micromonospora sp. strain 40027, a producer of the antibiotic fortimicin A. The autonomous system depends on the discovery of a low-copy-number, self-transmissible covalently closed circular plasmid, pJTU112 (ca. 14.1 kb), which was shown to be present in the progenitor strain in both integrated and autonomous states. The copy numbers of both wild-type pJTU112 and three derivatives of it can be amplified at least sixfold by addition of streptomycin to the culture medium. The integrative system was developed by the use of a pBR322-derived Escherichia coli plasmid vector, pSET152, mediated by the attP site of the Streptomyces phage ⌽C31. Both vectors can be transferred by conjugation from E. coli into Micromonospora sp. strain 40027. The heterologous cloning and expression of the dnd gene cluster originating from Streptomyces lividans 1326 into Micromonospora sp. strain 40027 demonstrated the use of the two systems.

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Overexpression of sgm 5’ UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells

Kojic¹,

Vojnović²,

Vukov³

et al. 2007

ARCH BIOL SCI BELGRA

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The 16S rRNA methylases are expressed by most of the antibiotic producing bacteria in order to protect themselves against antibiotics by methylation of 16S rRNA at positions which are crucial for their action. The sgm sisomicin-gentamicin resistance gene from Micromonospora zionensis methylates G1405 positioned in the A site of 16S rRNA, which includes a CCGCCC hexanucleotide. The same hexanucleotide is also present 14 nucleotides in front of the ribosome binding site of sgm mRNA. The model proposed for translational regulation of sgm assumes that Sgm binds to this motif, both on 16S rRNA and on the 5? untranslated region (UTR) of its own mRNA. The 5? UTR mRNA sequence was overexpressed on 3?-truncated sgm mRNA, and the effect on gentamicin resistance conferred by Sgm was tested in Escherichia coli and in Micromonospora melanosporea. Overexpression of the sgm mRNA regulatory region decreases the resistance to gentamicin in both E. coli and M. melanosporea. This effect is likely to be due to titration of Sgm molecules by the overexpressed 5? UTR.

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Analysis of plasmid pMZ1 fromMicromonospora zionensis

Cited by 3 publications

References 17 publications

Micromonospora

Micromonospora

Vector Systems Allowing Efficient Autonomous or Integrative Gene Cloning inMicromonosporasp. Strain 40027

Overexpression of sgm 5’ UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells

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