Analysis of Plasma microRNA Associated with Hemolysis

Shkurnikov, M. Yu.; Knyazev, E N; Fomicheva, K. A.; Михайленко, Д. С.; Nyushko, K. M.; Saribekyan, E. K.; Samatov, Timur R.; Alekseev, B. Ya.

doi:10.1007/s10517-016-3300-y

Cited by 35 publications

(32 citation statements)

References 12 publications

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“…Age and sex did not have effect on the miRNA expression profile, with no significantly differentially expressed miRNAs (DEmiRs), apart from one miRNA (eca-miR-8908j) that passed the lower significance threshold usually suggested for DEmiRs (upregulated in males with log 2 fold change (log2FC) = 4.23; FDR = 0.013). The absorbance at 414 nm had a significant effect on the expression of eca-miR-25 (FDR = 4.15e-4) and eca-miR-486-5p (FDR = 0.066) that was previously reported as haemolysis dependant [27]. We identified 50 differentially expressed (DE) miRNAs (42 known and 9 novel) between pony and Warmblood serum at a stringent significance threshold FDR < 0.001.…”

Section: Resultsmentioning

confidence: 99%

Novel equine tissue miRNAs and breed-related miRNA expressed in serum

et al. 2016

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BackgroundMiRNAs regulate multiple genes at the post-transcriptional level and therefore play an important role in many biological processes. It has been suggested that miRNA exported outside the cells contribute to inter-cellular communication. Consequently, circulating miRNAs are of particular interest and are promising biomarkers for many diseases. The number of miRNAs annotated in the horse genome is much lower compared to model organisms like human and mouse. We therefore aimed to identify novel equine miRNAs for tissue types and breed in serum.ResultsWe analysed 71 small RNA-seq libraries derived from nine tissues (gluteus medius, platysma, masseter muscle, heart, liver, cartilage, bone, total blood and serum) using miRDeep2 and miRdentify tools. Known miRNAs represented between 2.3 and 62.9 % of the reads in 71 libraries. A total of 683 novel miRNAs were identified. Breed and tissue type affected the number of miRNAs detected and interestingly, affected its average intensity. A total of 50 miRNAs in serum proved to be potential biomarkers to differentiate specific breed types, of which miR-122, miR-200, miR-483 were over-expressed and miR-328 was under-expressed in ponies compared to Warmbloods. The different miRNAs profiles, as well as the differences in their expression levels provide a foundation for more hypotheses based on the novel miRNAs discovered.ConclusionsWe identified 683 novel equine miRNAs expressed in seven solid tissues, blood and serum. Additionally, our approach evidenced that such data supported identification of specific miRNAs as markers of functions related to breeds or disease tissues.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3168-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Novel equine tissue miRNAs and breed-related miRNA expressed in serum

et al. 2016

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“…MiR-451 is a miRNA contained in red blood cells and its level is correlated with haemolysis; 14,22 it was not significantly altered by ECMO in our samples. Monitoring haemolysis, which is frequently observed during ECMO, 5 is critical when dealing with miRNAs because it is reported to be a significant source of variation in miRNA profiling.…”

Section: Discussionmentioning

confidence: 62%

Influence of extracorporeal membrane oxygenation on serum microRNA expression

et al. 2019

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Objective To date, no biomarkers have been established to predict haematological complications and outcomes of extracorporeal membrane oxygenation (ECMO). The aim of this study was to investigate the expression of a panel of microRNAs (miRNAs), which are promising biomarkers in many clinical fields, in patients before and after initiating ECMO. Methods Serum miRNA levels from 14 patients hospitalized for acute respiratory failure and supported with ECMO in our medical intensive care unit were analysed before and 24 hours after ECMO. In total, 179 serum-enriched miRNAs were profiled by using a real-time PCR panel. For validation, differentially expressed miRNAs were individually quantified with conventional real-time quantitative PCR at 0, 24, and 72 hours. Results Under ECMO support, platelet count significantly decreased by 65 × 103/µL (25th percentile = 154.3 × 103/µL; 75th percentile = 33 × 103/µL). Expression of the 179 miRNAs investigated in this study did not change significantly throughout the observational period. Conclusions According to our data, the expression of serum miRNAs was not altered by ECMO therapy itself. We conclude that ECMO does not limit the application of miRNAs as specific clinical biomarkers for the patients’ underlying disease.

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“…Both prior studies employed RNU6 as a reference. While hsa-miR-451a has been found to act as a tumor suppressor [64,65], it is also a marker of hemolysis [66] and thus we suggest that care should be employed to exclude samples with hemolysis before analysis. The miRNA ratio of hsa-miR-451a and hsa-miR-23a-3p has been employed by others [56,67] to monitor for sample hemolysis.…”

Section: Effect Of Reference Mirna Used To Normalize Resultsmentioning

confidence: 95%

microRNA and Overcoming the Challenges of Their Use in the Diagnosis of Endometriosis

Turpin¹,

Леонова²,

Agarwal³

et al. 2021

Endometriosis

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Endometriosis is a common estrogen dependent and inflammatory disease affecting approximately 176 million women worldwide. Currently, the time between onset of symptoms and a definitive diagnosis has been reported by several international studies to range from 6 to 12 years. Presently, laparoscopic surgery followed by histopathological confirmation of lesions remains the gold standard for diagnosis. In part because of cost and invasiveness, current trends favor reduced laparoscopic surgeries in preference of the non-surgical diagnosis of endometriosis. However, the search for a clinical marker or markers of endometriosis that provide equal or similar sensitivity and specificity to laparoscopy has remained elusive. Thus, the search for a diagnostic test for the diagnosis of endometriosis continues to be a high priority research and clinical issue. Recent studies have reported favorable results with microRNA; however, lack of replication and absence of validation suggest that circulating miRNA may not be reliable for clinical use. Use of different screening platforms together with divergent methods may account for some of the lack or reproducibility in the literature. Herein we critically assess the recent literature and explore sources for discrepant findings. We suggest that prospective studies using validated reference miRNA to normalize results together with improved study design may yet reveal a suitable diagnostic marker or panel of markers for the diagnosis of endometriosis.

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Analysis of Plasma microRNA Associated with Hemolysis

Cited by 35 publications

References 12 publications

Novel equine tissue miRNAs and breed-related miRNA expressed in serum

Novel equine tissue miRNAs and breed-related miRNA expressed in serum

Influence of extracorporeal membrane oxygenation on serum microRNA expression

microRNA and Overcoming the Challenges of Their Use in the Diagnosis of Endometriosis

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