Analysis of Plasma Citrulline and Intestinal Morphometry in Mice with Hepatosplenic Schistosomiasis

Siqueira, Luciana Teixeira de; Ferraz, A.; Campos, Josemberg Marins; Filho, José Luiz de Lima; Albuquerque, Mônica C.P.A.; Aires, André de Lima; Ribeiro, Maria Helena M. Lima; Cavalcanti, Maria Taciana Holanda; Cavalcanti, Carmelita de Lima Bezerra; Ferraz, Edmundo Machado

doi:10.1089/sur.2009.066

Cited by 5 publications

(4 citation statements)

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“…Concurrent infection with S. mansoni significantly altered the aforementioned anatomical sites mainly during chronic infection, irrespective of diet and the intestinal segment. Our results suggest that morphological alterations begin by week 9 and are aggravated by week 17, consistent with other studies of intestinal pathology (Borgers et al 2000;Couto et al 2002;Siqueira et al 2010). In this study, the damage to the intestinal tissue could be the result of inflammation (Trobojević-Stanković et al 2010) due to the higher number of eggs trapped in the intestine in mice fed a high-fat diet (Neves et al 2007, Alencar et al 2009.…”

Section: Discussionsupporting

confidence: 91%

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Changes in the small intestine ofSchistosoma mansoni-infected mice fed a high-fat diet

et al. 2012

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The consumption of a high-fat diet modifies both the morphology of the small intestine and experimentally tested effects of schistosomiasis mansoni. However, whether a schistosomiasis infection associated with a high-fat diet causes injury to the small intestine has never been investigated. Mice were fed either a high-fat or a standard-fat diet for 6 months and were then infected with Schistosoma mansoni cercariae. Physical characteristics of the intestinal tissue (mucosal thickness, small intestinal villi length and height, and abundance of goblet cells and enterocytes on the villous surface) and the distribution of granulomas along the intestinal segments and their developmental stage were measured at the time of sacrifice (9 or 17 weeks post-infection). The group fed a high-fat diet exhibited different granuloma stages, whereas the control group possessed only exudative granulomas. The chronically infected mice fed a high-fat diet exhibited higher granuloma and egg numbers than the acutely infected group. Exudative, exudative/exudative-productive and exudative-productive granulomas were present irrespective of diet. Computer-aided morphometric analysis confirmed that villus length, villus width, muscular height and submucosal height of the duodenal and jejunal segments were affected by diet and infection. In conclusion, a high-fat diet and infection had a significant impact on the small intestine morphology and morphometry among the animals tested.

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Section: Discussionsupporting

confidence: 91%

“…2002; Siqueira et al . 2010). In this study, the damage to the intestinal tissue could be the result of inflammation (Trobojević-Stanković et al .…”

Section: Discussionmentioning

confidence: 99%

Changes in the small intestine ofSchistosoma mansoni-infected mice fed a high-fat diet

et al. 2012

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“…A breakdown of the equilibrium of the autochthonous microbial population contributes to the selective overgrowth of some species, which may promote the translocation process (35) (36) . Immunomodulation might explain the alterations in microbial populations that occur during schistosomiasis.…”

Section: Discussionmentioning

confidence: 99%

Post-splenectomy infections in chronic schistosomiasis as a consequence of bacterial translocation

Lima

Negro-Dellacqua

Santos

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:Bacterial translocation is the invasion of indigenous intestinal bacteria through the gut mucosa to normally sterile tissues and internal organs. Schistosomiasis may cause alterations in the immune system and damage to the intestines, portal system and mesenteric lymph nodes. This study investigated bacterial translocation and alterations in the intestinal microbiota and mucosa in schistosomiasis and splenectomized mice. Methods: Forty female 35-day-old Swiss Webster mice were divided into the following four groups with 10 animals each: schistosomotic (ESF), splenectomized schistosomotic (ESEF), splenectomized (EF) and control (CF). Infection was achieved by introduction of 50 Schistosoma mansoni (SLM) cercariae through the skin. At 125 days after birth, half of the parasitized and unparasitized mice were subjected to splenectomy. Body weights were recorded for one week after splenectomy; then, the mice were euthanized to study bacterial translocation, microbiota composition and intestinal morphometry. Results: We observed signifi cant reductions in the weight increases in the EF, ESF and ESEF groups. There were increases of at least 1,000 CFU of intestinal microbiota bacteria in these groups compared with the CF. The EF, ESF and ESEF mice showed decreases in the heights and areas of villi and the total villus areas (perimeter). We observed frequent co-infections with various bacterial genera. Conclusions: The ESEF mice showed a higher degree of sepsis. This fi nding may be associated with a reduction in the immune response associated with the absence of the spleen and a reduction in nutritional absorption strengthened by both of these factors (Schistosoma infection and splenectomy).

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“…Surgical treatment of schistosomal portal hypertension by splenectomy with ligation of left gastric vein, followed by postoperative endoscopic sclerosis is indicated in cases associated with prior upper gastrointestinal bleeding due to rupture of esophageal varices and/or gastric ulcers, or in cases of hypersplenism with important clinical consequences for the patient 1 In an attempt to identify risk factors that may compromise the postoperative outcome of patients undergoing surgery for disconnection and splenectomy, several authors have tried to preoperatively identify parameters that can improve late results in this group of patients, mainly with respect to rebleeding [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] .…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Influência da inversão do diâmetro veia porta/veia esplênica nos resultados do tratamento cirúrgico da hipertensão portal esquistossomótica

Aa¹,

et al. 2011

Rev. Col. Bras. Cir.

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Results: 169 patients were analyzed, with follow-up averaging 23.6 months. Twenty-one patients (12.4%) had splenic vein caliber greater of equal than the portal vein's (Inversion -study group). The mean preoperative diameter of the portal and splenic veins were respectively 1.49 and 1.14 cm in the control group, and 0.98 versus 1.07 cm in the inversion group. The portal vein diameter was significantly higher in the control group when compared to the inversion group (p <0.05). Varices in the gastric fundus were found in 33.3% of the inversion group and in 38.5% of patients in the control group. Postoperative rebleeding occurred in 23.1% of patients in the inversion group and in 13.4% of the control group ones (p> 0.05). In the postoperative evaluation with Doppler ultrasonography of portal vessels, no cases of portal vein thrombosis were observed in the inversion group, whilst in the control group portal thrombosis was identified in 16.9% of the patients (p <0.05). Death occurred in one (4.8%) individual from the inversion group; mortality was 4.1% in the control group (p>0.05). The mean serum level of platelets was significantly lower (65,950/mm³) in the inversion group than in the controls (106,647/mm³) (p<0.05). Conclusion: Conclusion: Conclusion: Conclusion: Conclusion: The results suggest that the reversal of portal/splenic vein caliber ratio does not represent a contraindication to surgical treatment of schistosomal portal hypertension.

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Analysis of Plasma Citrulline and Intestinal Morphometry in Mice with Hepatosplenic Schistosomiasis

Abstract: Serum citrulline concentrations were reduced in mice with schistosomiasis, and a direct correlation was found between the citrulline concentration and the morphometry of the jejunal villi. Moreover, there was a reduction in the plasma concentration of citrulline after splenectomy.

Cited by 5 publications

References 45 publications

Changes in the small intestine ofSchistosoma mansoni-infected mice fed a high-fat diet

Changes in the small intestine ofSchistosoma mansoni-infected mice fed a high-fat diet

Post-splenectomy infections in chronic schistosomiasis as a consequence of bacterial translocation

Influência da inversão do diâmetro veia porta/veia esplênica nos resultados do tratamento cirúrgico da hipertensão portal esquistossomótica

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