Analysis of plant growth and biochemical parameters in Amsonia orientalis after in vitro salt stress

Acemi, Arda; Duman, Yonca Avcı; Karakuş, Yonca Yüzügüllü; Kömpe, Yasemin Özdener; Özen, Fazıl

doi:10.1007/s13580-017-0215-0

Cited by 12 publications

(7 citation statements)

References 36 publications

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“…This finding suggests a supplementary role of CAT in regulating H 2 O 2 in A. orientalis exposed to drought stress. A similar role of CAT was also observed during an in vitro salt stress in A. orientalis (Acemi et al, 2017b). The main function of CAT is to decompose mainly subcellular H 2 O 2 to H 2 O and O 2 .…”

Section: Discussionsupporting

confidence: 73%

“…Under long-term and/or extensive stress, the level of endogenous abscisic acid is increased while that of cytokinin is decreased to close the stomata, which limits water loss, whereas a short-term or mild stress could trigger a temporary elevation of cytokinin level and/or signaling (Ha et al, 2012). After in vitro salt treatment in A. orientalis, the lowest concentration of NaCl increased shoot numbers, which supports this hypothesis on shoot growth (Acemi et al, 2017b). However, shoot numbers remained statistically the same even after long-term drought in our study.…”

Section: Discussionsupporting

confidence: 64%

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Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.)

Acemi

Duman

Karakuş

et al. 2018

Folia Horticulturae

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This study aimed to investigate the effects of drought stress on Amsonia orientalis, an endangered ornamental plant with a limited natural distribution in Europe. Effects of polyethylene glycol (PEG)-mediated drought stress (-0.15, -0.49, -1.03 and -1.76 MPa osmotic potentials) were tested on in vitro cultures. In general, root lengths and numbers, total protein, chlorophyll a and carotenoid contents were negatively influenced at elevated levels of the stress factor. The successive decrease in the tested osmotic potentials resulted in gradually higher H2O2, malondialdehyde (MDA) and proline contents. Activities of the antioxidant enzymes, peroxidase (POD) and catalase (CAT), were found to be enhanced in response to the decreasing osmotic potential tested, whereas increased superoxide dismutase (SOD) activity was observed at the -0.15 MPa osmotic potential. Strong activation of POD enzymes under drought stress suggests that POD enzymes might have a major role in regulating the H2O2 content, while CAT has only a supplementary role in A. orientalis. These results indicated that although A. orientalis is susceptible to long-term drought, the species may survive during mild drought stress because the development of the plant was not totally inhibited but only limited. Nevertheless, the species should be introduced to well-irrigated lands, after evaluation of the soil’s water status, in order to ensure the continuation of its generations.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionsupporting

confidence: 64%

Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.)

Acemi

Duman

Karakuş

et al. 2018

Folia Horticulturae

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“…In a recent study on A. orientalis, it was shown that elevated POD activity and nearly unchanged CAT activity catalyzed the transformation of H 2 O 2 to H 2 O and protected the plant against drought stress (5). The increases in POD and CAT activities after Zn/Mo exposure were more pronounced than was found following salt and drought stresses in A. orientalis (4). The notable increases in POD but nearly unchanged activities of CAT after salt and drought stress suggested that CAT has only a supplementary role in H 2 O 2 regulation in A. orientalis.…”

Section: Discussionmentioning

confidence: 96%

“…Heavy metal exposure and accumulation can result in physiological and developmental disorders in plants. Reduced organ growth, limitations in photosynthesis due to reduced pigment synthesis and increased chloroplast deformation, over-production of antioxidant enzymes, accumulation of some osmoregulators, and increased lipid peroxidation are the most common signs of stress-related responses in plants (4,24).…”

Section: Discussionmentioning

confidence: 99%

“…The decrease in its habitat may be related to anthropogenic and/or environmental abiotic factors. To investigate the effects of salt and drought stress on the growth and physiology of A. orientalis, several studies have been conducted (4,5). However, the effects of heavy metal contamination, which is a major global environmental problem, on A.…”

Section: Introductionmentioning

confidence: 99%

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Effects of zinc and molybdenum on European Bluestar (Amsonia orientalis): An in vitro study

Acemi

Duman

Karakuş

et al. 2020

The EuroBiotech Journal

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This study aimed to investigate the effects of possible zinc (Zn) and molybdenum (Mo) contaminations on the critically endangered European Bluestar (Amsonia orientalis). The effects of Zn and Mo were tested in a dose-dependent manner on in vitro cultures. Zn at 0.1 mM in the medium inhibited root development whereas Mo showed the same effect only at ≥2.5 mM concentration. Gradual inhibition of shoot development was observed after treatment with both metals. Protein contents were also negatively affected by increasing metal concentrations, while proline levels increased gradually. Successive increases in metal concentrations resulted in higher hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations. The activity of the antioxidant enzymes, peroxidase (POD) and catalase (CAT), were found to be enhanced in response to increasing metal concentrations. Superoxide dismutase (SOD) activity decreased after Zn treatment but increased after Mo treatment. A marked increase in POD and CAT in response to metal stress suggests that these enzymes might have a significant cooperative role in regulating H2O2 production, although CAT, in response to drought and salt stress, has been reported to only play a supplementary role in A. orientalis. These results indicated that A. orientalis is susceptible to long-term Zn stress but can tolerate up to 2.5 mM Mo in the long-term. Deficiency of Mo is more common than high toxic concentrations in the environment. Therefore Zn contamination should be considered as one of the major threats for A. orientalis in its native habitat.

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Effect of salt stress on seed germination, morphology, biochemical parameters, genomic template stability, and bioactive constituents of Andrographis paniculata Nees

et al. 2021

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Analysis of plant growth and biochemical parameters in Amsonia orientalis after in vitro salt stress

Cited by 12 publications

References 36 publications

Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.)

Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.)

Effects of zinc and molybdenum on European Bluestar (Amsonia orientalis): An in vitro study

Effect of salt stress on seed germination, morphology, biochemical parameters, genomic template stability, and bioactive constituents of Andrographis paniculata Nees

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