Analysis of Plant Alkaloid Mixtures by Ammonia Chemical Ionization Mass Spectrometry

Alkaloids From Castilleja miniata and Penstemon whippleanus, Two Host Species For the Plume Moth, Amblyptilia (Platyptilia) pica

“…Routine data on instrumentation and general methods are available in a thesis (6). The crude alkaloid residue was also analyzed by NH3 ci gc/ms and these data (which corroborate the above work) have been published elsewhere (7). In addition, the gc-ms provided evidence for lupanine ( 7) and 1-acetyl-1,2,3,4-tetrahydropyridine (6).…”

“…13C Hydroxytetrahydrorhombifoline (5 .-Compound 5 appeared in tic (A1203, Et20, double developed) at an Rf lower than tetrahydrorhombifoline and higher than hydroxy lupanine. In the gc it appeared later than tetrahydrorhombifoline and before hydroxylupanine (7). It was not obtained pure except by gc/ms: ei ms (miz, rel.…”

“…The alkaloid is tentatively identified on this basis as the known 4-noractinidine 10 (12). The second minor alkaloid had MW 235 from the NH3 ci gc/ms (7). The molecular ion was not visible in the ei spectrum, but instead showed an miz 190 (M+-45).…”

“…value on tic than did tetrahydrorhombifoline and at a slightly greater retention time on gc. In the ei ms, tetrahydrorhombifoline itself shows no molecular ion peak but rather an M+-41, which represents loss of the N-allyl side chain, while NH3 ci ms (7) shows a high quasimolecular ion at mtz 249. These data and the fragmentation pattern are duplicated by 5, with the exception that the M+-41 in the ei ms and the quasimolecular ion in the NH3 ci ms are exactly 16 mass units higher.…”

The effects of Sceletium tortuosum in an in vivo model of psychological stress

“…Unfortunately no voucher specimen was lodged in a herbarium. Therefore, in order to put our study in context of the literature, we analysed the extract for its alkaloid content, using GC-MS techniques, based on the method described earlier (McCoy et al, 1983). Briefly, 1 ml chloroform and 1 ml 1 M HCl were added to 0.5 g plant material.…”