Analysis of pipecolic acid in biological fluids using capillary gas chromatography with electron-capture detection and [2H11]pipecolic acid as internal standard

Pre‐ and postnatal diagnosis of peroxisomal disorders using stable‐isotope dilution gas chromatography ‐ mass spectrometry

“…The second disadvantage arises from the use of the GC-MS equipment, which has high costs of acquisition and servicing. Therefore, for pipecolic acid determination we now frequently use a GC method with EC detection (Zee et al 1992). This method is less expensive but still yields high accuracy and sensitivity, owing to the use of the [2H~l]pipecolic acid internal standard.…”

“…Analysis of VLCFA in blood spots was performed according to Jakobs et al (1993), and of phytanic acid and pristanic acid in blood spots according to ten Brink et al (1993a). Analysis of pipecolic acid in body fluids was performed either according to Kok et al (1987) or to Zee et al (1992). The methods used to quantify bile acids in plasma and in amniotic fluid were described by Stellaard et al (1989Stellaard et al ( , 1991.…”

“…Levels of pipecolic acid in plasma, CSF, urine and amniotic fluid are summarized in Table 5. Concentrations of pipecolic acid in both plasma and urine of a healthy population were found to be age-dependent (Kok et al 1987;Zee et al 1992). Pipecolic acid is an intermediate in lysine degradation, and is generally found to be elevated in plasma and urine of patients affected by a peroxisomal deficiency disorder.…”

A new peroxisomal β‐oxidation disorder in twin neonates: Defective oxidation of both cerotic and pristanic acids

“…Immunoblot analysis of peroxisomal β-oxidation enzyme proteins in fibroblasts was performed according to Wanders and colleagues (1995d). Pipecolic acid was determined by a capillary gas chromatographic method with electron-capture detection using [ 2 H 11 ]pipecolic acid as internal standard (Zee et al 1992).…”

Diamond Paste Based Amperometric Biosensors for the Determination ofl- andd-Pipecolic Acid

“…1) is an important biological marker for the diagnosis of peroxisomal disorders (e.g., cerebrohepatorenal syndrome, neonatal adrenoleukodystrophy, infantile Refsum disease) while D-pipecolic acid (D-PA) ( Fig. Pipecolic acid and its derivatives were determined from biological samples using high performance liquid chromatography (HPLC), [5][6][7][8][9][10][11][12][13][14][15] gas chromatography, [16][17][18][19] liquid chromatography, [4,20] ion-exchange chromatography, [21] and electrophoresis techniques. [1][2][3][4] Many analytical methods have been reported to analyze pipecolic acid using expensive instrumentation and long sample preparation.…”