Analysis of photosynthetic pigments and chlorophyll fluorescence characteristics of different strains of Porphyra yezoensis

Tao, Zhang; Shen, Zonggen; Xu, Pao; Zhu, Jianyi; Lü, Quan; Shen, Yi; Wang, Yue; Yao, Chunyan; Li, Jiafu; Wang, Yunxia; Jiang, Hongxia

doi:10.1007/s10811-011-9708-x

Cited by 45 publications

(28 citation statements)

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“…Because relatively high photosynthetic physiological activity may accelerate the growth of the thallus, the study of the photosynthetic physiological characteristics of Pyropia strains is also important for the breeding of Pyropia (Zhang et al 2012(Zhang et al , 2014. From the perspective of breeding, it is important to breed a cultivar with high light-use efficiency.…”

Section: Discussionmentioning

confidence: 99%

“…Although there are many methods for seaweed breeding, mutagenesis remains the main breeding method. Some cultivars possessing good characteristics have been obtained through mutagenesis (Zhang et al 2012). Niwa et al (2011) selected a pigment mutant of P. yezoensis by heavy-ion beam mutagenesis that had higher contents of total FAA, Ala, and Tau than the wild type, and their study provided a good basis for Pyropia pigment mutant breeding.…”

Section: Discussionmentioning

confidence: 99%

“…The photosynthetic pigments were extracted according to Zhang et al (2012) with a slight modification. Five pieces of blade (exactly 1 cm 2 per piece) cut from vegetative tissues were thoroughly ground in 20 mL of sodium phosphate buffer (pH 6.8) and then kept in the dark for 12 h. After centrifugation for 20 min at 40,000×g, the phycobiliproteins (phycoerythrin (PE), phycocyanin (PC), and allophycocyanin (APC)) were extracted in the supernatant.…”

Section: Methodsmentioning

confidence: 99%

“…Our research has indicated that the measurement of photosynthetic parameters, such as chlorophyll fluorescence and photosynthetic pigment contents, can help to analyse photosynthetic characteristics of different Pyropia strains grown under stress environment, as these characteristics are closely related to the growth activity. Therefore, photosynthetic parameter measurement may be useful to improve Pyropia breeding efficiency (Zhang et al 2012). Chlorophyll fluorescence is commonly used in the study of photosynthesis, particularly PSII function (Schreiber et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Study on the photosynthetic characteristics of a Pyropia haitanensis red mutant cultured under different light intensities

Zhang

et al. 2015

J Appl Phycol

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The photosynthetic characteristics of a Pyropia haitanensis red mutant mutated from a wild-type strain using chemical mutagens were studied. The aim was to compare the differences in photosynthetic characteristics between the red mutant and the wild type, and then to analyse the possibility of the red mutant as breeding material. The results indicated that the cytoplasm thickness of the red mutant and the wild type were similar, whereas the cell wall of the red mutant was significantly thinner than that of the wild type. Additionally, the chlorophyll a extract absorption spectra of both strains exhibited no obvious difference, whereas the absorption peaks at 498, 545, and 565 nm (which belong to phycoerythrin, PE) in the phycobiliprotein extract absorption spectra of the red mutant were clearly higher than those of the wild type. The PE content per area of the red mutant thallus was significantly higher than that of the wild type, and the ratios of PE to phycocyanin (PC) and PE to chlorophyll a were significantly higher in the red mutant than in the wild type. The net photosynthetic oxygen evolution rates of the red mutant and wild type cultured under different light intensities demonstrated that the oxygen evolution rates of all samples decreased with increasing light intensities, whereas the oxygen evolution rate of the red mutant was higher than that of the wild type under the same light intensities. The light response curves also indicated that the light-saturated photosynthetic rates (P max ), light saturation points (LSPs), and light compensation points (LCPs) of both strains decreased to different degrees with increasing light intensities and that the P max and LSP values of the red mutant were significantly higher than those of the wild type under identical light intensities. In contrast, there were no obvious differences in the respiration rate (R d ) and in the LCP. The F v /F m values of both strains were similar before light treatment, whereas the F v /F m of the red mutants after culture under different light intensities (120 and 200 μmol photons m −2 s −1 ) for 3 days were significantly higher than those values of the wild type. Moreover, ΔF/F m ′ also exhibited a similar variation. Based on these results, the high content of PE in the P. haitanensis red mutant causes a steady red phenotype, and the increase in the PE concentration and the decrease in the thickness of the thallus may enhance its photosynthetic physiological activity. Thus, the red mutant of P. haitanensis may provide good material for Pyropia breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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