Analysis of phosphorous content in cancer tissue by synchrotron micro-XRF

Low Inorganic Phosphate Stress Inhibits Liver Cancer Progression: from In Vivo to In Vitro

“…The endothelial cell membrane is a physiological barrier to prevent interstitial Pi diffusion even intratumoral Pi concentration is higher than that of normal tissue and serum. [19,20] If this barrier has not been perturbed, intratumoral Pi will not be exhausted by sevelamer positioned in the blood vessels. As we confirmed, oral administra-tion of sevelamer only exhausts plasma Pi partially without deprivation of intratumoral Pi.…”

Study of anti-tumorigenic actions of essential fatty acids in a murine mammary gland adenocarcinoma by micro-XRF

“…They monitored the intensity excitation beam before and after it passed through the sample (and sample substrate) in order to calculate the transmission factor for each pixel at the mean energy E M of the polychromatic beam. By comparison with the mean transmission factor of a reference position, it is possible to calculate the transmission factor of the sample at each incident energy: 22 T ( E ) = e − μ S ( E ) ρ S t S Here ρ S is the sample density, t S is the sample thickness and μ S ( E ) is the mass attenuation coefficient for the sample at incident energy E .…”

“…The incoming microbeam excitation X-ray spectrum on the sample was determined through a spectral reconstruction technique, based on the scattering pattern of a thin standard plastic material. 22…”

“…[18][19][20] In addition, the phosphorous level can also be used to monitor the metabolic activity of the tumour cells since it plays an important role in the cancer growth. 21,22 Some authors have recently proposed inorganic phosphorous concentration in tumour microenvironment as a new marker of tumour progression. 23 There are other elements in biological tissues that are also accessible by micro-XRF and could be useful to obtain even more information of the tumour metabolism.…”

Atomic spectrometry update – a review of advances in X-ray fluorescence spectrometry and its special applications

“…Falchini et al 25 developed quantitative elemental imaging approaches for application to the important topic of cancer research. Their quantitative strategy was based on the fundamental parameter method which considered excitation by a polychromatic incident microbeam and took into account sample matrix effects.…”