Analysis of phenolic compounds in cork from Quercus suber L. by HPLC–DAD/ESI–MS

Fernandes, Ana; Sousa, André; Mateus, Nuno; Cabral, Miguel; Freitas, Víctor de

doi:10.1016/j.foodchem.2010.10.016

Cited by 88 publications

(69 citation statements)

References 33 publications

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“…Phenolics in each bark extract were identified by comparison of their retention times with corresponding standards and by their UV spectra obtained with the diode array detector – DAD (SPD-M20A). Gallic acid, vanillic acid, protocatechuic acid, chlorogenic acid, coumaric acid, ferulic acid, quercetin, and rutin were used as standard compounds (Prieto et al, 1999; Fernandes et al, 2011; Gómez-Caravaca et al, 2013). The linear regression equation for each standard curve was obtained by plotting the amount of standard compound injected against the peak area.…”

Section: Methodsmentioning

confidence: 99%

Commiphora leptophloeos Phytochemical and Antimicrobial Characterization

Pereira

Jandú

et al. 2017

Front. Microbiol.

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Commiphora leptophloeos is a plant specie usually known for its medicinal purposes in local communities in Northeast Brazil. In order to evaluate its therapeutic potential, we aimed to determine the phytochemical and antimicrobial properties of C. leptophloeos extracts. Thin Layer Chromatography (TLC) was able to detect the presence of phenolic compounds, flavonoids and reducing sugars. Three phenolic compounds were identified by HPLC and described as Gallic, Chlorogenic and Protocatechuic acids. On the other hand, H1NMR analysis revealed the presence of hinokinin, a bioactive lignan further characterized in the present work. The minimum inhibitory concentration (MIC) values for hinokinin ranged from 0.0485 to 3.125 mg/mL in different S. aureus clinical isolates and showed a bactericidal activity against MRSA isolated from blood (MMC 0.40 mg/mL) and postoperative secretion (MMC = 3.125 mg/mL). C. leptophloeos extracts also showed antimicrobial activity against Mycobacterium species such as M. smegmatis (MIC = 12.5 mg/mL) and M. tuberculosis (MIC = 52 mg/mL). Additionally, we determined the toxicity of C. leptophloeos by in vitro HC50 tests with hemolytic activity detected of 313 ± 0.5 μg/mL. Our results showed that C. leptophloeos possesses inhibitory properties against MRSA as well as several other clinically important microorganisms. Furthermore, the present work is the first report of the presence of hinokinin in Commiphora genus.

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Section: Methodsmentioning

confidence: 99%

Commiphora leptophloeos Phytochemical and Antimicrobial Characterization

Pereira

Jandú

et al. 2017

Front. Microbiol.

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“…2i UV spectrum is more likely (Arapitsas et al, 2007;Määttä-Riihinen et al, 2004). C-glycosidic ellagitannins have UV-Vis spectra with a maximum of around 245 nm (Fernandes, Sousa, Mateus, Cabral, & de Freitas, 2011). For compounds such as 2,3-(HHDP)-glucose, without a simple glucogalloyl ester, DAD have very low selectivity (Fig.…”

Section: Detection -Identificationmentioning

confidence: 99%

Hydrolyzable tannin analysis in food

2012

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“…Ellagic acid, gallic and protocatechuic acids have also previously been identified as the most abundant compounds in cork . Also reported was quinic acid, which is a metabolite of caffeoylquinic acid, and also coumarin brevifolin carboxylic acid and methyl gallate . Gallic acid was also reported as a typical pollutant present in the wastewaters of cork industry …”

Section: Resultsmentioning

confidence: 92%

“…6 Also reported was quinic acid, which is a metabolite of caffeoylquinic acid, and also coumarin brevifolin carboxylic acid and methyl gallate. 40,41 Gallic acid was also reported as a typical pollutant present in the wastewaters of cork industry. 42 Triterpene acid-O-hexoside, identified here, was reported for the first time in the cork wastewater (Table 5), but other triterpenes were also identified in cork processing industry byproducts, 43 as promising sources of bioactive chemicals or chemical intermediates for the synthesis of polymeric materials.…”

Section: Chromatographic Profile and Chemical Composition Of Wastewatmentioning

confidence: 99%

Cork processing wastewaters components fractioned by ultrafiltration membranes – studies of antioxidant and antitumoral activity

Gomes

Borges

Serralheiro

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Cork processing wastewater is an environmental problem due to its high content of organic matter, such as sugars and non‐biodegradable compounds such as polyphenols (PPs), namely tannins. Membrane technology aimed at valorisation of the wastewaters components, concentrate stream, and simultaneously offer a pre‐treatment of the wastewater, permeate stream. In this work, the identification of bioactive PPs was also envisaged. Several fractions of cork processing wastewaters were generated using two cellulose acetate ultrafiltration membranes of 3 kDa and 74 kDa for PPs isolation according to nature and molecular weights. The membranes were prepared by the phase inversion method and fractionation was made in concentration and diafiltration modes. The wastewater and the fractions were analyzed in terms of total organic carbon, total phenols, tannins and total polysaccharides content and the compounds present were identified by FTIR, LC–MS and quantified by HPLC‐DAD. RESULTS Compounds such as quinic, gallic, protocatechuic, brevifolin carboxylic and ellagic acids were identified as the major compounds in cork wastewater. The wastewater and the fractions were tested for antioxidant activity and for capacity to inhibit the proliferation of the growth of human breast carcinoma cell lines, MCF‐7. The fractions revealed high antioxidant activity with EC50 values ranging from 1.174 ± 0.069 to 1.943 ± 0.179 mg/mgDPPH. The fractions demonstrated to be efficient as cell proliferation inhibitors, with values of IC50 ranging from 0.20 ± 0.003 to 0.46 ± 0.02 mg mL‐1. CONCLUSION The process reported demonstrates that PPs compounds can be recovered from cork effluent and further reused as high‐value bioactive compounds. © 2017 Society of Chemical Industry

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Analysis of phenolic compounds in cork from Quercus suber L. by HPLC–DAD/ESI–MS

Cited by 88 publications

References 33 publications

Commiphora leptophloeos Phytochemical and Antimicrobial Characterization

Commiphora leptophloeos Phytochemical and Antimicrobial Characterization

Hydrolyzable tannin analysis in food

Cork processing wastewaters components fractioned by ultrafiltration membranes – studies of antioxidant and antitumoral activity

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