Analysis of Phenolic Compounds in Coix-seed Reactive Derivatives(CRD) by Using Liquid Chromatography/Mass Spectrometry(LC-MS)

信孝, 鈴木; 昌彦, 滝埜; 浩孝, 大桑

doi:10.1625/jcam.17.11

Cited by 2 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Calibration standard solutions were prepared by appropriate dilutions of the mixed stock solution. The sample solution was prepared as described previously [46]. Briefly, the CS extract (1.0 g) was weighed accurately and dissolved in 10 mL of ultrapure water (Wako).…”

Section: Preparation Of the Standard And Sample Solutionsmentioning

confidence: 99%

Coix Seed Extract Prevents Inflammation-mediated Skin Dryness Induced by Sodium Dodecyl Sulfate Exposure in HR-1 Hairless Mice

Nakanishi

Murata

et al. 2022

Planta Medica International Open

View full text Add to dashboard Cite

Skin inflammation and dryness are the features of surfactant-induced irritant contact dermatitis, a common skin disorder. In Japan, Coix seed (CS, Coix lacryma-jobi L. var. ma-yuen Stapf) is widely used as a traditional medicine and functional supplement to treat skin inflammation and dry skin. However, the efficacy of CS against surfactant-induced skin disorders has not been reported. Here, we investigated the effect of CS on inflammatory dry skin disorders induced by multiple topical applications of sodium dodecyl sulfate (SDS), a representative anionic surfactant. Male HR-1 hairless mice received a water extract of CS for four weeks. Three weeks after CS administration, the dorsal skin of the mice was exposed once daily to 10% SDS for five days. CS efficacy was then evaluated by measuring epidermal water content; erythema index; severity of skin scaling; epidermal thickness; inflammatory cell infiltration; production of pro-inflammatory mediators, such as interleukin-1α (IL-1α) and prostaglandin E2 (PGE2); and protein expression of cyclooxygenase 2 (COX-2), in the dorsal skin. Administration of CS markedly attenuated the SDS-induced reduction in epidermal water content, elevated erythema index, and severity of skin scaling. Histological analysis demonstrated that CS suppressed epidermal hyperplasia and macrophage infiltration in SDS-exposed skin. Furthermore, CS significantly prevented SDS-induced production of IL-1α and PGE2, as well as COX-2 upregulation. These results indicate that CS prevents SDS-induced inflammation-mediated skin dryness by inhibiting the production of pro-inflammatory mediators.

show abstract

Section: Preparation Of the Standard And Sample Solutionsmentioning

confidence: 99%