Analysis of phenolic compounds for determination of cambium differentiation and tracheal elements in olive graft combinations

Abstract

The aim of this investigation was to evaluate the effect of phenolic compounds during differentiation of cambium and tracheal elements in olive cultivars 'Ayvalik', 'Domat', 'Gemlik', 'Memecik', 'Nizip Yaglik' and 'Sari Ulak. These cultivars were grafted onto one year-old cv. 'Gemlik' rootstock. This rootstock has been propagated by cuttings. According to the results of histological studies for two years; new cambium cells were initiated in first three months while the formation of vascular bundles and sclerenchyma cells were initiated in six months. Large numbers of undifferentiated parancymatic cells were also determined in both side grafts of the cultivars 'Ayvalik', 'Domat' and 'Nizip Yaglik' after 3, 6and 12 months of grafting. Further, High levels of 4 hydroxyphenylacetic acid, vanillic and ferulic acids content were determined in the scion of the cultivars Ayvalik, Nizip Yağlik and Domat. As a result, during recovery of graft zone, development of new cambium tissues, vascular connections and sclerenchyma tissues occurred imperfectly in 'Ayvalik', 'Domat' and 'Nizip Yaglik' cultivars and graft zones of these combinations were found weak. Moreover level of 4Hydroxyphenylacetic acid and Ferulic acidphenolic compounds had high concentration in scions of 'Ayvalik' and 'Domat', respectively. These results illustrated existence of problem in differentiation of cambium and vascular systems in graft interface of 'Ayvalik' and 'Domat' cultivars.

Preliminary Evaluation of the Impact of Antioxidants Polyvinylpyrrolidone and Ascorbic Acid on Patch Budding of Persian Walnut

“…It should be noted that the percentage of budding success among different cultivars and genotypes is significantly different probably also because of differences in their phenolic materials content Vahdati & Zareie 2005;Karadeniz 2005). There are reports on the effect of high concentrations of phenolic substances on budding and grafting success (Mng'omba et al 2008;Azimi et al 2016). Karadeniz (2003) reported that there is a negative correlation between the amount of phenol and the percentage of graft success in the walnut.…”