Analysis of pH change and an automatic pH control with A new function: On-line estimation of acetic acid

Jung, Yoon Keun; Hur, Won

doi:10.1007/bf02932326

Cited by 1 publication

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Culture pH is a function of the governing chemical equilibrium and acid/base reactions, which generally occur very quickly, and therefore the overall system can be considered to be in equilibrium [10]. This equilibrium is disturbed by cell growth as a result of acid or base production, proton exchange across the cell membrane, and formation or consumption of components participating in equilibrium reactions [2,9,10]. The culture pH at the point of renewed equilibrium therefore depends on different parameters such as the rate of proton exchange and acid or base production, in addition to the buffer capacity of the medium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prediction of proton exchange and bacterial growth on various substrates using constraint-based modeling approach

Ehsan

2011

Biotechnol Bioproc E

View full text Add to dashboard Cite

Proton exchange between cells and medium is one of the most important factors affecting culture pH, and hence its prediction is advantageous. In this research, proton exchange flux across the cell membrane was predicted using a genome-scale model. Calculated proton exchange flux was then exploited as a criterion to predict the trends and intensities of pH changes in cultures of Bacillus subtilis containing various C-sources, i.e. glucose, sucrose, glycerol, lactate, and citrate, as well as N-sources, i.e. ammonium chloride, sodium nitrate, urea, and histidine. The results showed that glucose, sucrose, and glycerol lowered culture pH as compared to citrate and lactate, which raised it. With regard to N-sources, the model predicted that ammonium chloride lowered culture pH while other sources raised pH. Furthermore, maximum theoretical biomass yield using the various C&N-sources was calculated, and sensitivity of microbial growth to proton exchange flux was investigated using robustness analysis to identify the effect of pH on growth of B. subtilis using different substrates. Finally, the effect of ammonium nitrate, a widely used nitrogen source, on growth of B. subtilis was studied. Experimental data obtained by cultivation of B. subtilis DSM 3256 on mineral salt media containing various C&N-sources were used to confirm model predictions. Model predictions were in good agreement with the experimental results for all of the examined C-sources as well as ammonium chloride and sodium nitrate as N-sources. However, the predictions for the N-sources urea and histidine showed deviations, possibly because these two compounds serve as both C&N-sources.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Most bacteria grow best around neutral pH values (6.5 ~ 7.5), and hence pH control of the culture is essential in most fermentation processes to improve cell growth and product formation [1][2][3]. There are several experimental studies that discuss the significance of pH on growth and product formation in microorganisms [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Prediction of proton exchange and bacterial growth on various substrates using constraint-based modeling approach

Ehsan

2011

Biotechnol Bioproc E

View full text Add to dashboard Cite

show abstract

Analysis of pH change and an automatic pH control with A new function: On-line estimation of acetic acid

Cited by 1 publication

References 3 publications

Prediction of proton exchange and bacterial growth on various substrates using constraint-based modeling approach

Prediction of proton exchange and bacterial growth on various substrates using constraint-based modeling approach

Contact Info

Product

Resources

About