Analysis of pathogenicity and genetic variation among Phytophthora sojae isolates using RAPD

Mapping QTL tolerance to Phytophthora root rot in soybean using microsatellite and RAPD/SCAR derived markers

“…The collection, isolation, identiWcation, and puriWcation of P. sojae followed the procedure of Meng et al (1999). Plant materials were inoculated with P. sojae isolates from three locations in Northeastern China (JiXi, ShuangYaShan, and JianSanJiang) and one location in Canada (Woodslee) in the greenhouse.…”

Genetic variability of Phytophthora sojae isolates from Argentina

“…That study indicated a higher level of polymorphism than in the Argentinean isolates. Meng et al (1999) also found genetic diversity among isolates of P. sojae, with four distinct groups obtained by RAPD, with no relationships detected with geographic origin of isolates, the same finding of Peters et al (2005) with P. erythroseptica.…”

“…RAPD technology also has been used to confirm sexual recombination of P. sojae in vitro and to study the segregation of avirulence genes (Whisson et al 1994). Meng et al (1999) used RAPD markers to study populations of P. sojae from Indiana, Iowa and Minnesota and found no correlation of populations with geographic origin. Wang et al (2003) analyzed genetic diversity of isolates from China and the United States through RAPD and distinguished 12 genetic groups.…”

Development of quantification bioassays for live propagules of Phytophthora sojae in soil

“…Estimating oospore density using leaf disc baiting For leaf disc baiting (Meng et al 1999), 5 g of ground Mollisol (through 1 mm sieve) was placed in a 6 cm sterile Petri dish, and 3 mL of previously prepared oospore suspensions of Eps597-3 was added; the Petri dish was sealed with Parafilm (BEMIS, Neenah, WI, USA) and incubated at 24°C for 5 days with 12 h light/12 h dark to induce oospore germination. After incubation, the dish was flooded with 7 mL of an aqueous solution of penicillin (500 ppm), rifampicin (200 ppm), pentachloronitrobenzene (PCNB, 250 ppm) and carbendazim (250 ppm).…”

“…The leaf discs are then placed on selective medium to isolate P. sojae (Canaday and Schmitthenner 1982;Meng et al 1999;Schmitthenner et al 1994). Although this leaf disc baiting method is the primary technique for isolating live P. sojae from infested soil and estimating the number of propagules in soil samples, it is not accurate enough for studies on the ecology and epidemiology of P. sojae in the soil.…”