Analysis of oxygen isotopes of inorganic phosphate (δ18Op) in freshwater: A detailed method description for obtaining oxygen isotopes of inorganic phosphate in environmental water samples

Nisbeth, Catharina S.; Tamburini, Federica; Kidmose, Jacob; Jessen, Søren; O’Connell, David

doi:10.1016/j.mex.2022.101706

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…It is essential to collect all crystals as fractionation occurs during precipitation, producing a final isotopically inhomogeneous precipitate with δ 18 O values varying over several tenths of a per mil [ 5 ]. Other studies have found that if crystal formation does not initiate, it could be due to the pH of the solution or that the conditions of supersaturation were not attained [ 17 ]. The TCA solution used for plant material has the optimal pH value of around 1 for APM formation and when restraining the sample volume prior to the precipitation, crystal formation was always successful.…”

Section: Step Iii: Apm Precipitation and Dissolutionmentioning

confidence: 99%

“…It is important to perform a cation exchange step to remove cations that could interfere with the final precipitation of silver phosphate, like residual Mg 2+ or Na + ions [18] . The cation exchange step can be performed in column or batch mode [ 3 , 4 , 17 ] as long as all counter ions are removed properly by allowing enough equilibration time with the resin and the phosphate is quantitatively collected. This column setup provided a satisfactory flowrate (ml/min).…”

Section: Step V: Cation Exchange Stepmentioning

confidence: 99%

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An optimized method for extraction and purification of inorganic phosphate from plant material for oxygen isotope ratio analysis

Rieckmann,

Blake,

Chang

et al. 2024

MethodsX

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Section: Step Iii: Apm Precipitation and Dissolutionmentioning

confidence: 99%

Section: Step V: Cation Exchange Stepmentioning

confidence: 99%

An optimized method for extraction and purification of inorganic phosphate from plant material for oxygen isotope ratio analysis

Rieckmann,

Blake,

Chang

et al. 2024

MethodsX

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“…The extract preparation for isotope analysis used a modified protocol developed by Nisbeth et al (2019), which produces silver phosphate (Ag 3 PO 4 ) crystals (details are in the S1.4 Text). To adapt the protocol for soil matrix extracts, an organic matter removal step (DAX-8) and a cation removal step were added to the protocol, as described in Tamburini et al (2010).…”

Section: Extractable Soil P Isotopic Analysismentioning

confidence: 99%

Phosphorus availability and speciation in soils amended with upcycled dairy-waste nutrients

Laan,

Strawn,

Kayler

et al. 2024

Front. Chem. Eng.

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The excessive application of dairy manure to soils to supply plant nutrients can result in increased offsite nutrient transport and degraded water quality. However, by concentrating nutrients from dairy-waste onto biochar or as biosolids, a viable alternative fertilizer can be produced that will benefit soil health, promote carbon sequestration, and decrease nutrient leaching into surface waters. In this study, a greenhouse experiment was conducted to assess soil phosphorus (P) speciation and barley plant growth in soils amended with dairy-waste treated biochar and fermented manure. Phosphorus characterization in the post-harvest soils was determined using selective extractions, 31P-NMR and XANES spectroscopy, and isotopic tracing (P-δ18O). Selective extractions and NMR spectroscopy revealed that most of the P in the amended soils occurred as inorganic species (>85%). XANES spectroscopy further showed that the soil P occurred as either calcium-P minerals (54%–87%) or adsorbed P (0%–46%) species. Analysis by P-δ18O in water and sodium bicarbonate extractions of the treated soils showed that the water-extracted P pool is cycled much faster than the sodium bicarbonate extracted P. Although less than 10% of the total P in the dairy-derived bioproducts was extracted using water, plant productivity in the soils treated with these amendments was the same as that in soils treated with equivalent amounts of conventional nitrogen and P fertilizer. This suggests that dairy-derived bioproducts are good soil amendments to supply nutrients and limit leaching.

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“…To be able to analyse the δ 18 O P from such a diverse range of substrates, several obstacles had to be overcome. The main obstacles for the δ 18 O P method are low P concentrations and/or high concentrations of interfering compounds like organic matter (Tamburini et al 2010;Goldhammer et al 2011b;Nisbeth et al 2019). Therefore, modern protocols of the δ 18 O P method involve several purification steps before the final precipitation of silver phosphate (Ag 3 PO 4 ) which is then used to determine the δ 18 O P via TC/EA-IRMS.…”

Section: From Past To Presentmentioning

confidence: 99%

The Use of the δ18OP to Study P Cycling in the Environment

Pfahler

Adu-Gyamfi

O’Connell

et al. 2022

Oxygen Isotopes of Inorganic Phosphate in Environmental Samples

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Phosphorus (P) fertilizers are known to increase crop productivity; however, when applied in excess, it can cause serious environment pollution. Monitoring P pollution in natural environments using stable isotopes has been difficult because P has only one stable isotope (31P) making the use of P stable isotope tracing not an option. Radioactive P isotopes (32P and 33P) have been used but its drawbacks are the short half-life, health risks and safety procedures required to apply them in agricultural catchments. Phosphorus in organic and inorganic P forms is strongly bonded to oxygen (O), which has three stable isotopes, providing a system to track P cycling in agricultural catchments and environment using the stable isotopes of O in phosphate (δ18O-PO4). In recent years, various studies have indicated that the analysis of the stable isotopic composition of oxygen (O) bound to P (δ18Op) to better understand P cycling in the environment, has become a promising tracer (surrogate) to investigate soil P transformation, plant P uptake and to trace the sources of P from the soil to water bodies and the environment. The chapter outlines the background and examples of δ18Op studies in sediments, soils, fresh water, mineral fertilizers and plants.

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Analysis of oxygen isotopes of inorganic phosphate (δ18Op) in freshwater: A detailed method description for obtaining oxygen isotopes of inorganic phosphate in environmental water samples

Cited by 4 publications

References 37 publications

An optimized method for extraction and purification of inorganic phosphate from plant material for oxygen isotope ratio analysis

An optimized method for extraction and purification of inorganic phosphate from plant material for oxygen isotope ratio analysis

Phosphorus availability and speciation in soils amended with upcycled dairy-waste nutrients

The Use of the δ18OP to Study P Cycling in the Environment

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