Analysis of oxybutynin and N-desethyloxybutynin in human urine by dispersive liquid–liquid microextraction (DLLME) and capillary electrophoresis (CE)

Moreira, Bruna Juliana; Borges, Keyller Bastos; Oliveira, Anderson Rodrigo Moraes de; Gaitani, Cristiane Masetto de

doi:10.1039/c5ay01641j

Cited by 8 publications

(5 citation statements)

References 50 publications

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“…In CE, the separation voltage is also an important parameter for controlling electro‐osmosis . The separation voltage of 15, 17, 19, 20, and 22 kV was checked by using a background eletrolyte solution consisting of 20 mmol/L Na 2 B 4 O 7 ·10H 2 O, and 10% v/v ACN at a pH value of 9.80.…”

Section: Resultsmentioning

confidence: 99%

Dispersive liquid–liquid microextraction of five chlorophenols in water samples followed by determination using capillary electrophoresis

et al. 2018

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Dispersive liquid-liquid microextraction (DLLME) coupled with CE was developed for simultaneous determination of five types of chlorophenols (CPs), namely 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), 2,6-dichlorophenol (2,6-DCP), and 2,4,6-trichlorophenol (2,4,6-TCP) in water samples. Several parameters affecting DLLME and CE conditions were systematically investigated. Under the optimized DLLME-CE conditions, the five CPs were separated completely within 7.5 min and good enrichment factors were obtained of 40, 193, 102, 15, and 107 for 4-CP, 2,4,6-TCP, 2,4-DCP, 2-CP, and 2,6-DCP, respectively. Good linearity was attained in the range of 1-200 μg/L for 2,4,6-TCP, 2,4-DCP, 2-300 μg/L for 4-CP and 2-CP, and 1-300 μg/L for 2,6-DCP, with correlation coefficients (r) over 0.99. The LOD (S/N = 3) and the LOQ (S/N = 10) were 0.31-0.75 μg/L and 1.01-2.43 μg/L, respectively. Recoveries ranging from 60.85 to 112.36% were obtained with tap, lake, and river water spiked at three concentration levels and the RSDs (for n = 3) were 1.31-11.38%. With the characteristics of simplicity, cost-saving, and environmental friendliness, the developed DLLME-CE method proved to be potentially applicable for the rapid, sensitive, and simultaneous determination of trace CPs in complicated water samples.

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Section: Resultsmentioning

confidence: 99%

Dispersive liquid–liquid microextraction of five chlorophenols in water samples followed by determination using capillary electrophoresis

et al. 2018

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“…The literature comprises different methods for the determination of OXB either alone, in combination with other drugs or in presence of its degradation product. Some of these methods include: spectrophotometric methods [3][4][5], HPLC [6][7][8], gas chromatography [9,10], thin layer chromatography [5], capillary electrophoresis [11] and potentiometric methods [12][13][14][15]. To the best of our knowledge, only one voltammetric method has been applied for the analysis of OXB using differential pulse and square wave cathodic adsorptive stripping voltammetry on mercury electrode surface [16].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Oxybutynin Hydrochloride Utilizing Pencil Graphite Electrode Decorated with Gold Nanoparticles

et al. 2022

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A novel voltammetric method was successfully applied for the determination of an anticholinergic drug, oxybutynin hydrochloride (OXB). The method is concerned with electrooxidation of the drug on the surface of pencil graphite electrode (PGE). In order to enhance the electrode sensitivity and peak current, the electrode was coated with gold nanoparticles (Au-NPs) via electrochemical deposition using cyclic voltammetry from gold salt solution. The surface of Au-NPs modified PGE has been characterized using scanning electron microscopy and X-ray photoelectron spectroscopy. Various experi-mental variables were studied and optimized to enhance the sensor's response towards OXB. Quantitative determination of the drug was achieved in phosphate buffer pH 7.5 using differential pulse voltammetry by scanning the potential over range of 0.00 to 2.20 V with scan rate of 40 mV s À 1 . Validation of the method was achieved according to ICH guidelines. The method was found to be linear over concentration range (2.0 × 10 À 7 -1.0 × 10 À 6 M). The suggested sensor was efficiently developed for the quantitative determination of OXB in pure form, pharmaceutical dosage form and spiked plasma samples.

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“…But compared with capillary electrophoresis (CE), HPLC has the disadvantages of consuming a large amount of organic solvent, long analysis time and more sample usage [16,17]. As a result, CE has become the preferred alternative method with high resolution, rapid analysis, low sample and reagent consumption, and flexible separation modes [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Determination of hexachlorophene in cosmetics by capillary electrophoresis compared with high performance liquid chromatography

Gao

Huitao

et al. 2020

AChrom

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Abstract A simple capillary electrophoresis (CE) method with ultraviolet (UV) detection was developed for the determination of hexachlorophene (HCP) in cosmetics. Separation conditions were obtained in 20 mM Na2B4O7, 10% MeOH (pH 9.20), with 25 kV applied voltage and UV detection at 208 nm. Under the selected conditions, electrophoretic analysis was completed in about 4 min, with limit of detection (LOD) of 0.06 µg·mL−1 for HCP. The method was successfully applied to determine HCP in three kinds of cosmetics with relative standard deviations (RSD) of 0.52–3.02% and recoveries from 90.0 to 96.4% for the spiked samples. The results indicated that the proposed method was reliable. Comparative experiments were also carried out with high-performance liquid chromatography (HPLC)-UV method described in National Standards of People's Republic of China. The validation results of the two methods are comparable, but the proposed CE method is simple, rapid, which makes separation and analyte quantification in shorter time with much less reagent consumption.

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Analysis of oxybutynin and N-desethyloxybutynin in human urine by dispersive liquid–liquid microextraction (DLLME) and capillary electrophoresis (CE)

Abstract: A DLLME/CE method was developed to the determination of oxybutynin (OXY) and N-desethyloxybutynin (DEO) in human urine.

Cited by 8 publications

References 50 publications

Dispersive liquid–liquid microextraction of five chlorophenols in water samples followed by determination using capillary electrophoresis

Dispersive liquid–liquid microextraction of five chlorophenols in water samples followed by determination using capillary electrophoresis

Voltammetric Determination of Oxybutynin Hydrochloride Utilizing Pencil Graphite Electrode Decorated with Gold Nanoparticles

Determination of hexachlorophene in cosmetics by capillary electrophoresis compared with high performance liquid chromatography

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