Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

Sandesc, Mihai; Rogobete, Alexandru Florin; Bedreag, Ovidiu Horea; Dinu, Anca; Păpurică, Marius; Cradigati, Carmen Alina; Sărăndan, Mirela; Popovici, Sonia Elena; Bratu, Lavinia Melania; Bratu, Tiberiu; Stan, Adrian Tudor; Săndesc, Dorel

doi:10.17305/bjbms.2018.2306

“…Numerous species of microRNAs are involved in the pathogenesis of mitochondrial damage, these being responsible for the excess production of oxygen free radicals (OFRs), as well as for endothelial and vascular dysfunctions. [16][17][18][19][20][21][22][23] Magenta et al have investigated the effects and implications of OFRs on the epigenetic mechanisms of miRNAs. 24 Oxidative stress (OS), as well as increased production of OFRs have important implications in the pathogenesis of CVDs through the ischemia and ischemia-reperfusion (IR) syndrome injury they can cause.…”

Section: The Most Common Molecular Mechanisms Involved In Cvdsmentioning

confidence: 99%

Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

Buzaș

¹

,

Rogobete

²

,

Popovici

³

et al. 2018

Journal of Cardiovascular Emergencies

Self Cite

View full text Add to dashboard Cite

Worldwide, cardiovascular diseases (CVDs) represent one of the main causes of morbidity and mortality, and acute coronary syndromes are responsible for a large number of sudden cardiac deaths. One of the main challenges that still exist in this area is represented by the early detection and targeted monitoring of the pathophysiology involved in CVDs. During the last couple of years, researchers have highlighted the importance of molecular and epigenetic mechanisms involved in the initiation and augmentation of CVDs, culminating in their most severe form represented by acute myocardial infarction. One of the most studied molecular factors involved in this type of pathology is represented by nuclear transcription factor kappa B (NF-κB), as well as the involvement of microRNAs (miRNAs). It has been suggested that miRNAs can also be involved in the complex process of atheromatous plaque vulnerabilization that leads to an acute cardiac event. In this review paper, we describe the most important molecular mechanisms involved in the pathogenesis of CVDs and atheromatous plaque progression and vulnerabilization, which include molecular mechanisms dependent on NF-κB. For this paper, we used international databases (PubMed and Scopus). The keywords used for the search were “miRNAs biomarkers”, “miRNAs in cardiovascular disease”, “NF-κB in cardiovascular disease”, “molecular mechanism in cardiovascular disease”, and “myocardial NF-κB mechanisms”. Numerous molecular reactions that have NF-κB as a trigger are involved in the pathogenesis of CVDs. Moreover, miRNAs play an important role in initiating and aggravating certain segments of CVDs. Therefore, miRNAs can be used as biomarkers for early evaluation of CVDs. Furthermore, in the future, miRNAs could be used as a targeted molecular therapy in order to block certain mechanisms responsible for inducing CVDs and leading to acute cardiovascular events.

show abstract

“…In hypermetabolic states like inflammation and sepsis, the oxidant/antioxidant balance is significantly disrupted, with an exaggerated increase in FR expressions. This major imbalance that becomes systemic in a very short period of time is called oxidative stress (OS), and is involved in the majority of biochemical pathways [90][91][92][93][94][95].…”

Section: Cannabinoid Signaling System and Redox Activity-linked With mentioning

confidence: 99%

“…As a consequence, numerous fragments resulting from the mtDNA destruction will be released, which will further interact with the immune system. This cascade of molecular interactions will lead to the activation of macrophages, neutrophils, and TLR-cells, with a further augmentation of the inflammatory status and acceleration of the cellular redox stress [95][96][97][98][99][100][101].…”

Section: Cannabinoid Signaling System and Redox Activity-linked With mentioning

confidence: 99%

Cannabis Sativa Revisited—Crosstalk between microRNA Expression, Inflammation, Oxidative Stress, and Endocannabinoid Response System in Critically Ill Patients with Sepsis

Dinu

¹

,

Rogobete

²

,

Bratu

³

et al. 2020

Cells

Self Cite

View full text Add to dashboard Cite

Critically ill patients with sepsis require a multidisciplinary approach, as this situation implies multiorgan distress, with most of the bodily biochemical and cellular systems being affected by the condition. Moreover, sepsis is characterized by a multitude of biochemical interactions and by dynamic changes of the immune system. At the moment, there is a gap in our understanding of the cellular, genetic, and molecular mechanisms involved in sepsis. One of the systems intensely studied in recent years is the endocannabinoid signaling pathway, as light was shed over a series of important interactions of cannabinoid receptors with biochemical pathways, specifically for sepsis. Furthermore, a series of important implications on inflammation and the immune system that are induced by the activity of cannabinoid receptors stimulated by the delta-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) have been noticed. One of the most important is their ability to reduce the biosynthesis of pro-inflammatory mediators and the modulation of immune mechanisms. Different studies have reported that cannabinoids can reduce oxidative stress at mitochondrial and cellular levels. The aim of this review paper was to present, in detail, the important mechanisms modulated by the endocannabinoid signaling pathway, as well as of the molecular and cellular links it has with sepsis. At the same time, we wish to present the possible implications of cannabinoids in the most important biological pathways involved in sepsis, such as inflammation, redox activity, immune system, and epigenetic expression.

show abstract

“…As a consequence, numerous fragments resulting from the mtDNA destruction will be released, which will further interact with the immune system. This cascade of molecular interactions will lead to the activation of macrophages, neutrophils, and TLR-cells, with a further augmentation of the inflammatory status and acceleration of the cellular redox stress [95][96][97][98][99][100][101].…”

Section: Cannabinoid Signaling System and Redox Activity-linked With mentioning

confidence: 99%

Cannabis Sativa Revisited - Crosstalk between microRNAs Expression, Inflammation, Oxidative Stress and Endocannabinoid Response System in Critically Ill Patients with Sepsis

Rogobete¹,

Bratu²,

Bedreag³

et al. 2019

Preprint

Self Cite

0

View full text Add to dashboard Cite

Critically ill patients with sepsis require a multidisciplinary approach, as this situation implies multiorgan distress, with most of the bodily biochemical and cellular systems being affected by the condition. Moreover, sepsis is characterized by a multitude of biochemical interactions and by dynamic changes of the immune system. At the moment, there is a gap in our understanding of the cellular, genetic, and molecular mechanisms involved in sepsis. One of the systems intensely studied in recent years is the endocannabinoid signaling pathway, as light was shed over a series of important interactions of cannabinoid receptors with biochemical pathways, specifically for sepsis. Furthermore, a series of important implications on inflammation and the immune system that are induced by the activity of cannabinoid receptors stimulated by the delta-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) have been noticed. One of the most important is their ability to reduce the biosynthesis of pro-inflammatory mediators and the modulation of immune mechanisms. Different studies have reported that cannabinoids can reduce oxidative stress at mitochondrial and cellular levelsThe aim of this review paper was to present, in detail, the important mechanisms modulated by the endocannabinoid signaling pathway, as well as of the molecular and cellular links it has with sepsis. At the same time, we wish to present the possible implications of cannabinoids in the most important biological pathways involved in sepsis, such as inflammation, redox activity, immune system, and epigenetic expression. cascade and in the evolution of sepsis are represented by the inflammatory response, immune response, and redox activity, followed by the involvement of cellular defects, which all lead to severe organ dysfunction. Adding to this phenomenon are severe respiratory infections; tissue hypoxia; acute kidney injury (AKI); metabolic imbalances; and, last but not least, derangements of the biological system as a whole. Through the augmentation of the inflammatory status and through the concomitant derangement of several organ functions, a high percentage of critical patients with sepsis develop multiple organ dysfunction syndrome (MODS), and finally death [8,[10][11][12].Numerous biochemical and cellular systems involved in sepsis pathogenesis have been studied, both in order to better understand these complex mechanisms, and in the hope of finding new therapeutic targets. The endocannabinoid response system is one of them. Recent studies have shown the involvement of specific endocannabinoid receptors, such as endocannabinoid CB1 receptor and CB2 receptor, as well as their link with important processes in sepsis, such as the immune response, inflammatory response, and redox activity [13]. Furthermore, a series of implications on the epigenetic processes have also been proven, through the change in the microRNAs expression, the microRNAs responsible for the modulation of the immune, and inflammatory systems [14]. It was also shown that by stimulatin...

show abstract

Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

Cited by 29 publications

References 25 publications

Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

Cannabis Sativa Revisited—Crosstalk between microRNA Expression, Inflammation, Oxidative Stress, and Endocannabinoid Response System in Critically Ill Patients with Sepsis

Cannabis Sativa Revisited - Crosstalk between microRNAs Expression, Inflammation, Oxidative Stress and Endocannabinoid Response System in Critically Ill Patients with Sepsis

Contact Info

Product

Resources

About