Analysis of oxidative stress expressed by urinary level of biopyrrins and 8‐hydroxydeoxyguanosine in patients with chronic schizophrenia

Miyaoka, Tsuyoshi; Ieda, Masa; Hashioka, Sadayuki; Wake, Rei; Furuya, Motohide; Liaury, Kristian; Hayashida, Maiko; Tsuchie, Keiko; Arauchi, Ryosuke; Araki, Toshimitsu; Shioji, Izuru; Ezoe, Satoko; Inoue, Ken; Yamaguchi, Tokio; Horiguchi, Jun

doi:10.1111/pcn.12319

Cited by 18 publications

(17 citation statements)

References 37 publications

(49 reference statements)

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“…No significant difference was observed in 8OHdG levels (P ¼ 0.26). In patients with chronic schizophrenia, a recent report has shown an increase in the UBP level, but no increase in the urinary 8OHdG level [30]. This finding suggests that patients with chronic schizophrenia are under a certain kind of oxidative stress, and our results also suggest that the UBP level is more associated with school stress than the 8OHdG level.…”

Section: Discussionsupporting

confidence: 67%

Reference urinary biopyrrin level and physiological variation in healthy young adults: relation of stress by learning

Tada

Shiota

Hayashi³

et al. 2020

Heliyon

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Background: Biopyrrins are end products of oxidation reactions of bilirubin with reactive oxygen, and urinary biopyrrin (UBP) levels might increase under oxidative stress. The authors examined the reference UBP level for healthy adults and its physiological variation in 40 healthy volunteers recruited from among students of our university (20 students each from third-year and fourth-year), and compared the results with data on 8-hydroxy-2deoxyguanosine (8OHdG). Methods: UBP and 8OHdG levels could be considered as oxidative stress markers. The UBP levels were measured with a competitive ELISA kit using biopyrrin antibody 24G7, according to the manufacturer's protocol. 8OHdG levels were measured with a Highly Sensitive 8-OHdG Check kit. UBP and 8OHdG measurements were performed in triplicate and means values calculated. For both parameters, creatinine (Cr) correction was performed using urinary creatinine levels measured by an enzymatic method. Results: A comparison of the UBP levels between different grades revealed that the third-year students under high stress from clinical training and other course work tended to have a higher UBP level than fourth-year students. Therefore, we compared the current UBP levels in fourth-year students (samples collected in 2018) with their UBP level when they were in the third-year (samples collected in 2017) to examine the annual change. We found that the UBP level in 2017 samples was significantly higher than that in 2018 samples (P < 0.05). No difference in the 8OHdG level. Additionally, no effect of menstrual stress on the UBP level was observed. Conclusions: These results suggest that the UBP levels may be related to school-related stress and menstruation has no effects on urinalysis results.

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Section: Discussionsupporting

confidence: 67%

Reference urinary biopyrrin level and physiological variation in healthy young adults: relation of stress by learning

Tada

Shiota

Hayashi³

et al. 2020

Heliyon

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“…Biopyrrins were found to be correlated with rating scores 69,71 and response to treatment, 70 as well as the duration of the disorder. 71 The variation in the oxidative metabolism of bilirubin logically indicates the differences in the antioxidant activity of the molecule. However, this could mean the increased recruitment of healthy antioxidant defenses or be the result of compensation by the UCB-ROS buffer system, as previously discussed.…”

Section: Discussionmentioning

confidence: 95%

“…Miyaoka et al 71 published an article in which the urinary BOMs of 29 patients with schizophrenia (DSM-IV-TR) were compared with those of 30 healthy controls. The mean levels of urinary BOMs in patients with schizophrenia and normal controls were 1.51 ± 0.74 and 1.11 ± 0.54 μmol/g creatinine, respectively; the mean levels of urinary BOMs in patients with schizophrenia were significantly higher than those of the control subjects (P = 0.019).…”

Section: High Levels Of Ucb In Humans With Schizophreniamentioning

confidence: 99%

Unconjugated bilirubin and schizophrenia: a systematic review

2019

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Schizophrenia is a complex syndrome of unknown etiology and difficult to manage. Unconjugated bilirubin has been researched as a potential biological marker of this syndrome. The objective of this review article was to gather the studies published to date on the relationship between this molecule and schizophrenia. Broad inclusion criteria have been used (PRISMA) to include as many relevant studies as possible. Fourteen studies were selected: 3 analyzed the effects of unconjugated hyperbilirubinemia in animal models; 6 demonstrated an increased incidence of schizophrenia in patients with increased unconjugated bilirubin; 2 reported an increased incidence of the disease in patients with decreased unconjugated bilirubin; and 3 linked an increased incidence of schizophrenia with an increased excretion of the oxidative product of bilirubin, the so-called biopyrrins. Because of apparently contradictory reported results, the hypothesis that the relationship between schizophrenia and unconjugated bilirubin was not linear and that there was an inflammatory dysfunction explaining this was considered. The 2 most accepted models for the pathophysiology of schizophrenia are described, and the possible role of the molecule in each is clarified. The bilirubin buffer system and its role in antioxidant defense was explored. The average levels of unconjugated bilirubin in patients with schizophrenia, schizoaffective disorder, and bipolar disorder were also compared, having been hypothesized that these diseases could be different points of a same pathological spectrum. Finally, it was concluded that unconjugated bilirubin is a promising molecule that could be used as a possible biological marker for schizophrenia, and the necessity of subsequent efforts for its research was considered.

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“…The level of urinary 8-OHdG was measured with a MICRO Plate READER Model 680 (Bio-Rad Laboratories Inc., Hercules, CA, USA) by using an 8-OHdG Check ELISA kit, which contains the anti-8-OHdG monoclonal antibody N45.1 (Japan Institute for the Control of Aging). 14 The results of 8-OHdG were corrected for the urinary concentration of creatinine, which was determined with an Accuras Auto CRE (Shino test).…”

Section: Methodsmentioning

confidence: 99%

Natural reduced water suppressed anxiety and protected the heightened oxidative stress in rats

Masuda

Tanaka

Kanehisa

et al. 2017

NDT

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BackgroundIn Japan, the effects of reduced water, such as hydrogen-rich electrolyzed reduced water and natural reduced water, like Hita Tenryosui water®, have been examined. The purpose of the present study was to identify the role of natural reduced water in anxiety and blood biochemical analysis.Materials and methodsNatural reduced water and distilled water were administered to rats for 180 consecutive days, and their effect on anxiety-like behavior and depression was examined by using elevated plus maze, light/dark, forced swimming, and conditioned fear tests. Before and after administration of natural reduced or distilled water, we performed blood and urine analyses.ResultsNatural reduced water exhibited anxiolytic-like effects in the conditioned fear and elevated plus maze tests. The mean levels of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the natural reduced water were significantly lower than the distilled water group. Natural reduced water group also showed decrease in blood–urea nitrogen levels compared with the distilled water group.ConclusionThese results indicate that natural reduced water may decrease anxiety-related behaviors and prevent heightened oxidative stress.

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Analysis of oxidative stress expressed by urinary level of biopyrrins and 8‐hydroxydeoxyguanosine in patients with chronic schizophrenia

Cited by 18 publications

References 37 publications

Reference urinary biopyrrin level and physiological variation in healthy young adults: relation of stress by learning

Reference urinary biopyrrin level and physiological variation in healthy young adults: relation of stress by learning

Unconjugated bilirubin and schizophrenia: a systematic review

Natural reduced water suppressed anxiety and protected the heightened oxidative stress in rats

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