Analysis of Oxidative DNA Damage in Patients with Colorectal Cancer

Ribeiro, Marcelo Lima; Priolli, Denise Gonçalves; Miranda, Daniel D. C.; Arçari, Demétrius Paiva; Pedrazzoli, José; Martinez, Carlos Augusto Real

doi:10.3816/ccc.2008.n.034

Cited by 51 publications

(56 citation statements)

References 9 publications

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“…When they are produced in excess, ROS are harmful to cells and damage proteins, membranes, organelles and nucleotide bases, which can results in genetic mutations 25,26,27,28,29 .…”

Section: Discussionmentioning

confidence: 99%

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Reduction in oxidative stress levels in the colonic mucosa without fecal stream after the application of enemas containing aqueous Ilex paraguariensis extract

Cunha¹,

Silva

Almeida

et al. 2011

Acta Cir. Bras.

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PURPOSE: To evaluate the antioxidant effects of enemas containing aqueous extract of Ilex paraguariensis, comparing segments with and without fecal stream and correlating the segments with the duration of intervention. METHODS: Twenty-six Wistar rats were subjected to a diversion of the fecal stream in the left colon by a proximal colostomy and distal mucosal fistula. The rats were distributed randomly into two experimental groups of 13 animals each based on the time of sacrifice after surgical procedure (two or four weeks). Each group was then divided into two experimental subgroups that received either second daily enemas containing 0.9% saline solution or aqueous extract of Ilex paraguariensis at 0.2g/100g. Colitis was diagnosed by histopathological analysis and the detection of oxidative tissue damage by measuring the levels of malondialdehyde. The Mann-Whitney test was used to compare the tissue levels of malondialdehyde between colon segments with and without fecal stream in each experimental group, and the Kruskal-Wallis test was used to verify the variance between the levels of oxidative stress according the duration of the irrigation; both tests determined significance at 5% (p<0.05). RESULTS: The levels of malondialdehyde in the animals subjected to intervention in the colon with saline with and without fecal stream after two and four weeks of irrigation were 0.05±0.006 and 0.06±0.006, and 0.05± 0.03 and 0.08 ±0.02, respectively. The malondialdehyde levels in the animals irrigated with Ilex paraguariensis with and without fecal stream after two and four weeks of irrigation were 0.010±0.002 and 0.02±0.004, and 0.03±0.007 and 0.04±0.01, respectively. After two and four weeks of intervention, the levels of malondialdehyde were lower in the animals irrigated with Ilex paraguariensis regardless of the time of irrigation (p=0.0001 and p=0.002, respectively). CONCLUSION: The daily rectal application of enemas containing aqueous extract of Ilex paraguariensis decreases oxidative tissue damage in the colon without fecal stream regardless of the time of irrigation.

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“…When they are produced in excess, ROS are harmful to cells and damage proteins, membranes, organelles and nucleotide bases, which can results in genetic mutations 25,26,27,28,29 .…”

Section: Discussionmentioning

confidence: 99%

“…Cells have developed defense mechanisms to maintain the balance between ROS production and neutralization and avoid oxidative stress 12,[25][26][27] . The colon mucosa is particularly vulnerable to oxidative stress 23 .…”

Section: Discussionmentioning

confidence: 99%

Reduction in oxidative stress levels in the colonic mucosa without fecal stream after the application of enemas containing aqueous Ilex paraguariensis extract

Cunha¹,

Silva

Almeida

et al. 2011

Acta Cir. Bras.

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“…Studies also demonstrate that combination of 5-ASA with other antioxidants such as n-acetylcysteine produces better clinical and histological responses when compared with 5-ASA alone, suggesting that the capacity of reducing ROS in tissues is proportional to the increased antioxidant activity [53][54][55][56]. By such actions, 5-ASA can decrease or completely inhibit the oxidative DNA damage known to be involved in pathogenesis of UC, DC, and colitis-associated CRC [25,43,[58][59][60][61]. Although 5-ASA has been used in patients with UC, few studies have evaluated its therapeutic effects in patients with DC [40,41].…”

Section: Discussionmentioning

confidence: 99%

5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

et al. 2010

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“…Being formed in DNA by specific chemical mechanisms, such adducts may lead to mutations in proto-oncogenesis or tumour suppressor genes, and they start the process of carcinogenesis (Lehman et al 1994, Kinzler et al 1996. It is well established that inflammation facilitates the progression of normal cells to malignant cells, the production of pro-inflammatory cytokines such as TNF, IL-1, IL-6, IL-23 and reactive oxygen species (ROS) and nitrogen (Bartsch et al 2006, Roessner et. al.…”

Section: Inflammatory Bowel Disease and Carcinogenesismentioning

confidence: 99%