Analysis of opiates in urine using microextraction by packed sorbent and gas Chromatography- Tandem mass spectrometry

Simão, Ana Y.; Monteiro, Catarina; Marques, Hernâni; Rosado, Tiago; Margalho, Cláudia; Barroso, Mário; Andraus, Maristela Haddad; Gallardo, Eugénia

doi:10.1016/j.jchromb.2022.123361

Cited by 7 publications

(2 citation statements)

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“…The reconstitution of the sorbent was modified as well, and the following conditions were used: 2 cycles of 250 µL of 1% NH 4 OH in methanol: acetonitrile (1:1), then 2 cycles of 250 µL of 1% formic acid in isopropanol: water (10:90). Following unsuccessful experiments, it was decided to try another method based on that by Simão et al [ 26 ] that used the same biological specimen, again with unsatisfactory outcomes.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, the quantities of solvents and samples are significantly reduced in MEPS [ 18 , 19 , 20 , 21 ]. This approach, in combination with techniques like GC–MS, is a powerful tool for screening and for the determination of several compounds in biological samples [ 19 , 20 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Additionally, MEPS can be fully automated when coupled online, without any adjustments [ 20 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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The Determination of Cannabinoids in Urine Samples Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry

et al. 2022

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Cannabis is the most consumed illicit drug worldwide, and its legal status is a source of concern. This study proposes a rapid procedure for the simultaneous quantification of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH), cannabidiol (CBD), and cannabinol (CBN) in urine samples. Microextraction by packed sorbent (MEPS) was used to pre-concentrate the analytes, which were detected by gas chromatography–mass spectrometry. The procedure was previously optimized, and the final conditions were: conditioning with 50 µL methanol and 50 µL of water, sample load with two draw–eject cycles, and washing with 310 µL of 0.1% formic acid in water with 5% isopropanol; the elution was made with 35 µL of 0.1% ammonium hydroxide in methanol. This fast extraction procedure allowed quantification in the ranges of 1–400 ng/mL for THC and CBD, 5–400 ng/mL for CBN and 11-OH-THC, and 10–400 ng/mL for THC-COOH with coefficients of determination higher than 0.99. The limits of quantification and detection were between 1 and 10 ng/mL using 0.25 mL of sample. The extraction efficiencies varied between 26 and 85%. This analytical method is the first allowing the for determination of cannabinoids in urine samples using MEPS, a fast, simple, and low-cost alternative to conventional techniques.

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Section: Resultsmentioning

confidence: 99%