Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species

Nina, Jaime; McDonald, Vincent; Dyson, D.A.; Catchpole, J.; Uni, Shigehiko; Iseki, Motohiro; Chiodini, P. L.; Mcadam, K. P. W. J.

doi:10.1128/iai.60.4.1509-1513.1992

Cited by 36 publications

(10 citation statements)

References 20 publications

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“…When antigens obtained from C. muris oocysts and sporozoites are used in immunoblotting, bands at 110 kDa, 54-51 kDa, and in the region of low molecular weights have been found (Nina et al, 1992) , unlike our finding of sporozoite antigens with bands at 78, 47.5, and 27.5 kDa. C. parvum sporozoites have surface antigens of 55, 28, 20, and 15 kDa that may be responsible for induction of the neutralization antibodies (Mead et al, 1988;Riggs et al, 1989;Tilley et al, 1991) .…”

Section: Infectivity Of Sporozoites Treated With Antiserumcontrasting

confidence: 97%

Neutralization of Cryptosporidium muris Sporozoites by Rabbit Anti-C. muris Serum.

Uni

Hayashi

Fukunaga

et al. 1995

Jap. J. Trop. Med. Hyg.

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Section: Infectivity Of Sporozoites Treated With Antiserumcontrasting

confidence: 97%

Neutralization of Cryptosporidium muris Sporozoites by Rabbit Anti-C. muris Serum.

Uni

Hayashi

Fukunaga

et al. 1995

Jap. J. Trop. Med. Hyg.

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“…Furthermore, some C. parvum isolates have been shown to infect chicks when large numbers of oocysts are used for inoculation (20,34). The results of an analysis of an undefined DNA sequence (39) and an antigen reactivity analysis (23) also suggested that C. parvum and C. baileyi are more closely related to each other than they are to C. muris. The results of this study also suggest that Cryptosporidium parasites are not related to other coccidians at the SSU rRNA locus.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Cryptosporidium Parasites Based on the Small-Subunit rRNA Gene Locus

Xiao

Escalante

Yang

et al. 1999

Appl Environ Microbiol

649

263

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Biological data support the hypothesis that there are multiple species in the genus Cryptosporidium, but a recent analysis of the available genetic data suggested that there is insufficient evidence for species differentiation. In order to resolve the controversy in the taxonomy of this parasite genus, we characterized the small-subunit rRNA genes of Cryptosporidium parvum,Cryptosporidium baileyi, Cryptosporidium muris, and Cryptosporidium serpentis and performed a phylogenetic analysis of the genus Cryptosporidium. Our study revealed that the genus Cryptosporidium contains the phylogenetically distinct species C. parvum, C. muris, C. baileyi, and C. serpentis, which is consistent with the biological characteristics and host specificity data. The Cryptosporidium species formed two clades, with C. parvum and C. baileyi belonging to one clade and C. muris and C. serpentisbelonging to the other clade. Within C. parvum, human genotype isolates and guinea pig isolates (known asCryptosporidium wrairi) each differed from bovine genotype isolates by the nucleotide sequence in four regions. A C. muris isolate from cattle was also different from parasites isolated from a rock hyrax and a Bactrian camel. Minor differences were also detected between C. serpentis isolates from snakes and lizards. Based on the genetic information, a species- and strain-specific PCR-restriction fragment length polymorphism diagnostic tool was developed.

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“…The lack of an efficient in vitro culturing system for this parasite (5) still represents a serious obstacle to understanding the biology of the parasite and to studying the immunological and biochemical properties of Cryptosporidium antigens. In fact, until recently, few parasite proteins have been extensively characterized (1,(14)(15)(16) and very few genes have been cloned (4,6,9). There is an urgent need to collect more information on the biology of Cryptosporidium proteins.…”

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confidence: 99%

Characterization and immunolocalization of a Cryptosporidium protein containing repeated amino acid motifs

et al. 1993

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The oocyst wall is one of the components that permits cryptosporidia both to survive in the environment and to retain infectivity. With the aim of identifying Cryptosporidium proteins specifically expressed at the oocyst stage, we screened Agtll genomic libraries of Cryptosporidium parvum with both an oocyst antiserum and a * Corresponding author.We thought that such genes would be important both in understanding how the parasites survive and retain infectivity for several months in the external environment and in searching for the antigens that may be the targets of the immune response. To generate Xgtll expression libraries of cryptosporidia, we used either DNA extracted from purified oocysts or DNA derived from the gut cells of the brush border of a Cryptosporidium parvum-infected calf. For this purpose, we developed a method for collecting the infected cells of the superficial layer of the mucosa, thus increasing the parasite DNA/intestinal cell DNA ratio. On the basis of the reactivity with a Cryptosporidium oocyst antiserum, we selected from the library insert cpRL3, which was generated with the DNA extracted from infected gut cells. This insert encoded a partial sequence of 786 amino acids. The analysis of the deduced amino acid sequence suggested that the Cryptosporidium protein may play an important functional and/or structural role in the parasite. Using cpRL3 as a hybridization probe, we isolated from a library generated with shared DNA of Cryptospondium oocysts an insert of 4,420 bp. This insert encompassed the entire sequence of cpRL3 and contained an open reading frame of 1,252 amino acids. The deduced amino acid sequence was characterized by a high content of cysteine and by the presence of two distinct repeated amino acid motifs. We developed sera and monoclonal antibodies against a recombinant polypeptide encompassing the first 786 amino acids to study the expression and localization of the protein in C. parvum. MATERIALS AND METHODS Developmentof a Agtll library with DNA extracted from infected gut mucosa. To develop a C. parvum genomic 2347 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from 2348 RANUCCI ET AL. on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species

Cited by 36 publications

References 20 publications

Neutralization of Cryptosporidium muris Sporozoites by Rabbit Anti-C. muris Serum.

Neutralization of Cryptosporidium muris Sporozoites by Rabbit Anti-C. muris Serum.

Phylogenetic Analysis of Cryptosporidium Parasites Based on the Small-Subunit rRNA Gene Locus

Characterization and immunolocalization of a Cryptosporidium protein containing repeated amino acid motifs

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