Analysis of oligoguluronic acids with NMR electrospray ionization–mass spectrometry and high-performance anion-exchange chromatography

Liu, Yan; Jiang, Xiaolu; Liao, Wei; Guan, Huashi

doi:10.1016/s0021-9673(02)00849-x

Cited by 24 publications

(7 citation statements)

References 12 publications

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“…11 In order to express the structure conveniently, 'D' was used to represent the non-reducing end residue, 'M' stood for middle residue near non-reducing end and 'R' represented for reducing end residue. The chemical shifts of H-4 (5.76 ppm) and C-4 (108.90 ppm) of the non-reducing end were found in down-field in 1 H NMR and 13 C NMR spectra, while the non-reducing end H-1 and reducing end anomeric carbon (C-1) of mannuronic acid residues appeared at Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…High resolution of 1 H and 13 C using NMR techniques are primary, rapid, and efficient technology for determining the structure of oligosaccharide and sequence determination of polysaccharides. [10][11][12][13][14][15] Here, we used extracellular endo-alginate lyase from Pseudomonas sp. HZJ216 to hydrolyze algae alginate, and the oligosaccharide fractions were separated by anion exchange chromatography.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Li¹,

Jiang²,

Guan³

et al. 2011

Carbohydrate Research

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Li¹,

Jiang²,

Guan³

et al. 2011

Carbohydrate Research

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“…One unit of enzyme activity was defined as the amount of enzyme required to cause an increase of one unit of optical density at 235 nm. 10 The reaction was carried out at 28°C for 24 h, and then the solution was heated to 100°C and kept for 10 min to stop the hydrolysis reaction. Ethanol was added to the solution until the content of ethanol reached 50% (v/v), then the mixture was centrifuged to get rid of the precipitate.…”

Section: Preparation Of Alginate Oligosaccharidesmentioning

confidence: 99%

Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510

Zhang¹,

Yu²,

Guan³

et al. 2004

Carbohydrate Research

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Abstract-Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri-and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.

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“…For the enzymatic production of AOS, the substrate specificity and catalytic mechanism of alginate lyase can be elucidated using NMR (Vilen, Lundqvist, Jouanneau, Helbert, & Sandstrom, ). However, NMR spectra can be difficult to elucidate where AOS composition is complex (Liu et al., ). Individual highly enriched AOS isomers are often required to enable unequivocal NMR analysis.…”

Section: Separation and Characterization Of Aosmentioning

confidence: 99%

Alginate Oligosaccharides: Production, Biological Activities, and Potential Applications

Liu

Yang

et al. 2019

Comp Rev Food Sci Food Safe

212

121

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Alginate, a group of polyuronic saccharides, has been widely used in both pharmaceutical and food industries due to its unique physicochemical properties as well as beneficial health effects. However, the potential applications of alginate are restricted because of its low water solubility and high solution viscosity when significant concentrations are needed, particularly in food products. Alginate oligosaccharides (AOS), oligomers containing 2 to 25 monomers, can be obtained via hydrolysis of glycosidic bonds, organic synthesis, or through biosynthesis. Generally, AOS have shorter chain lengths and thus improved water solubility when compared with higher molecular weight alginates of the same monomers. These oligosaccharides have attracted interest from both basic and applied researchers. AOS have unique bioactivity and can impart health benefits. They have shown immunomodulatory, antimicrobial, antioxidant, prebiotic, antihypertensive, antidiabetic, antitumor, anticoagulant, and other activities. As examples, they have been utilized as prebiotics, feed supplements for aquaculture, poultry, and swine, elicitors for plants and microorganisms, cryoprotectors for frozen foods, and postharvest treatments. This review comprehensively covers methods for AOS production from alginate, such as physical/chemical methods, enzymatic methods, fermentation, organic synthesis, and biosynthesis. Moreover, current progress in structural characterization, potential health benefits, and AOS metabolism after ingestion are summarized in this review. This review will discuss methods for producing and modified AOS with desirable structures that are suited for novel applications.

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Analysis of oligoguluronic acids with NMR electrospray ionization–mass spectrometry and high-performance anion-exchange chromatography

Cited by 24 publications

References 12 publications

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510

Alginate Oligosaccharides: Production, Biological Activities, and Potential Applications

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