Analysis of Old Samples of Cannabis sativa L.

Abstract

Relatively few phytochemical studies have been conducted on very old samples of Cannabis sativa L., particularly leaf and stem material [1–4]. At least one article [5] has shown that the fluid extract of marihuana is stable for as long as 40 years. A more recent article [6] reports on the stability of cannabinoids in stored plant material at different temperatures.

Potent Inhibition of Oxidative Phosphorylation by Marine Natural Products

“…For example, metyrapone is used to inhibits steroid 11-P-hydroxylase, but it also is a potent inhibitor of many other cytochrome P-450 transformations (1,3).…”

“…pressants, smooth muscle-novel marine compounds Respiration, mitochondrial-effect of marine natural products Der Marderosian (1) observed that marine organisms offered enormous potential for the development of new and different pharmaceuticals, and he cited examples of the pharmacological activities from marine sources known a t that time. In addition, Der Marderosian stressed the difficulties in handling the crude extracts for biological screening and the problems inherent in the extraction and characterization of the compounds responsible for the biological activity.…”

“…Keyphrases 0 Oxidation-cytochrome P-450, inhibition of in uitro metabolism of aniline and aminopyrine, effect of substituted pyridines on inhibition 0 Aniline-hydroxylase activity, effect of substituted pyridines on in uitro aniline metabolism, cytochrome P-450 oxidation reactions Aminopyrine-demethylation activity, effect of substituted pyridines on in uitro aminopyrine metabolism, cytochrome P-450 oxidation reactions Cytochrome P-450 is the terminal oxygenase responsible for the metabolism of many drugs and xenobiotics. Numerous pyridine-containing compounds such as metyrapone and ellipticine are known to inhibit cytochrome P-450-catalyzed reactions (1,2). It has been suggested that potent inhibitors of cytochrome P-450-catalyzed oxidations may be useful therapeutically in the prevention of chemical-induced cancer (2).…”

“…The nonselective nature of the known inhibitors of cytochrome P-450-mediated reactions limits their therapeutic application. For example, metyrapone is used to inhibits steroid 11-P-hydroxylase, but it also is a potent inhibitor of many other cytochrome P-450 transformations (1,3).…”