Analysis of nutrition-relevant trace elements in human blood and serum by means of total reflection X-ray fluorescence (TXRF) spectroscopy

Stosnach, Hagen; Mages, Margarete

doi:10.1016/j.sab.2009.03.019

Cited by 44 publications

(28 citation statements)

References 9 publications

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“…To reduce the sample thickness, the dilution of sample with ultrapure water is used. Sample preparation conditions for direct TXRF of biological liquids vary widely depending on the sample matrix and dry matter content, for instance, the aliquot deposited on the sample career ranges from 3 µl to 22 µl, the dilution ratio (sample: dilutant) is from 1:1 (for serum) up to 1:10 (for blood) …”

Section: Introductionmentioning

confidence: 99%

Choice of optimal conditions for X‐ray fluorescence analysis of milk products with varying fat content

Smagunova

Pashkova

2013

X-Ray Spectrometry

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The design of experiments was used to study sources of errors in the sample preparation and to choose optimal conditions for X‐ray fluorescence (XRF) analysis of milk products of varying fat content. The measurements were performed using the conventional wavelength‐dispersive XRF (WDXRF) and the total reflection XRF (TXRF) techniques. For WDXRF, the dried milk samples were pelletized, and the mathematical models were constructed, which described the dependence of XRF intensity on the pelletizing pressure, the pellet weight and the milk fat content. The effect of radiation time on the stability of pressed milk powder samples was also estimated. When applying TXRF, the sample preparation involved diluting milk samples with the ultrapure water, adding the internal standard (Ga) and drying of a sample aliquot on a quartz glass sample carrier. The mathematical models were designed, which described the dependence of the sample preparation error on the dilution ratio and sample aliquot pipetted on the carrier. A physical interpretation of the obtained mathematical models was proposed. Copyright © 2013 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Choice of optimal conditions for X‐ray fluorescence analysis of milk products with varying fat content

Smagunova

Pashkova

2013

X-Ray Spectrometry

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“…In contrast, total reflection X-ray fluorescence (TXRF) does not suffer from these matrix effects because of its lack of sensitivity for light elements and as long as the sample is small and can be dried as a homogenous residue. Studies investigating various elements in human body fluids and tissues showed excellent results (Von Bohlen et al, 1988;Marco et al, 1999;Hernandez-Caraballo and Marco-Parra, 2003;Varga et al, 2005;Stosnach and Mages, 2009). Surprisingly little is known about the presence and concentration of heavy metals in ocular tissue and how it influences vision and acuity.…”

Section: Heavy Metal Analysis In Lens and Aqueous Humor Of Cataract Pmentioning

confidence: 99%

Heavy metal analysis in lens and aqueous humor of cataract patients by total reflection X-ray fluorescence spectrometry

2014

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(2014). Heavy metal analysis in lens and aqueous humor of cataract patients by total reflection X-ray fluorescence spectrometry . Powder Diffraction, 29, pp 155-158. doi:10.1017/ S0885715614000281. Heavy Metal Analysis In Lens And Aqueous Humor Of Cataract Patients By Total Reflection X-Ray Fluorescence SpectrometryMartina Schmeling, Bruce I. Gaynes, And Susanne Tidow-Kebritchi The human eye is continuously exposed to the environment yet little is known about how much of toxins, specifically heavy metals are present in its different parts and how they influence vision and acuity. To shed light into this subject, aqueous humor and lens samples were collected from 14 cataract patients to study the presence and concentration of selected metals in the eye. Subjects undergoing routine cataract surgery were consecutively enrolled for study by simple random sampling. Prior to surgery, subject demographic were compiled. The surgical procedure involved small incision cataract removal using phacoemulsification. During the procedure, a small aliquot of aqueous humor was retained for analysis, whereas homogenized lens fragments were obtained during phacoemulsification. A balanced salt solution was used as control for each set of samples. Both ocular specimens were analyzed by total reflection X-ray fluorescence spectrometry after dilution and addition of an internal standard. The data obtained show substantial variations in elemental signature between the two media (aqueous humor and lens) and the patients themselves. Most commonly found heavy metals in both types of media were chromium and manganese. Barium was found in the lens, but not in aqueous tissue, whereas nickel was found only in the aqueous humor. Concentrations were generally higher in aqueous samples. Further study and increased sample size are required to more accurately elucidate the relationship between systemic and ocular metal accumulation and the impact of metal accumulation on measures of visual function and ocular disease.

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“…PicoTAX, S2 PICOFOX, and NanoHunter, are used in laboratories and for field investigations instead of large‐scale TXRF systems with high‐power X‐ray tubes and liquid‐nitrogen cooled detectors. For instance, the S2 PICOFOX spectrometer, equipped with an air‐cooled low‐power X‐ray tube and a liquid nitrogen‐free silicon drift detector, has been applied to the multi‐element analysis of waste water effluents, landfill leachates, blood and serum, lichens, pharmaceuticals, and minerals . The good analytical results have been obtained by using the combination of the portable spectrometer and rapid microextraction procedure for the toxic element determinations in environmental water samples …”

Section: Introductionmentioning

confidence: 99%

Study of factors affecting the results of natural water analyses by total reflection X‐ray fluorescence

2013

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The study aims to choose the conditions for the direct analysis of different types of natural water samples by total reflection X-Ray fluorescence spectrometry (TXRF). The effect of some factors on TXRF results was studied such as the surface density of dried water residue on the sample carrier, the dilution ratio of high-mineralized samples with ultrapure water and the solution of the detergent Triton X-100, the salt contents, the internal standard concentration, and repeated pipetting of an aliquot of fresh water. The influence of self-absorption phenomena on quantification results was demonstrated by using brine samples. The TXRF results for natural waters of varying salinity (brines, fresh waters, and ground waters) and model solutions are presented. For a number of analytes, the data obtained by TXRF were compared to those obtained by 'wet' chemistry and inductively coupled plasma mass spectrometry.

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Analysis of nutrition-relevant trace elements in human blood and serum by means of total reflection X-ray fluorescence (TXRF) spectroscopy

Cited by 44 publications

References 9 publications

Choice of optimal conditions for X‐ray fluorescence analysis of milk products with varying fat content

Choice of optimal conditions for X‐ray fluorescence analysis of milk products with varying fat content

Heavy metal analysis in lens and aqueous humor of cataract patients by total reflection X-ray fluorescence spectrometry

Study of factors affecting the results of natural water analyses by total reflection X‐ray fluorescence

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