Analysis of nitrofurans residues in foods of animal origin

Molognoni, Luciano; Daguer, Heitor; Hoff, Rodrigo Barcellos

doi:10.1016/b978-0-12-822360-4.00015-7

Cited by 2 publications

(2 citation statements)

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“…These substances are broad spectrum antimicrobial agents, with genotoxic and carcinogenic properties (EFSA 2015) and therefore prohibited. The NFs rapidly metabolise to 3-amino-2-oxazolidinone (AO Z), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AHD), semicarbazide (SEM) and 3,5-dinitrosalicylic acid hydrazide (DNSH), respectively (Molognoni et al 2021). For that reason, instead of the native substances, the metabolites are included in the analyses of NFs in food products as mentioned in the guideline created by the European advisory body, the European Reference Laboratory (EURL) (CRL 2007).…”

Section: Introductionmentioning

confidence: 99%

“…However, multiple methods for the analyses of NFs together with other substance classes are limited not only in milk but also in all kind of matrices (Perez et al 2002;Xia et al 2008;Shen et al 2013;Kaufmann et al 2015;Shendy et al 2016;Zhang et al 2017;Aldeek et al 2018;Chen et al 2020). For NFs analysis, a hydrolysis is required to include protein-bound residues and a derivatisation is applied to stabilise the metabolites and to enhance the mass spectrometric signal (Molognoni et al 2021). However, using these hydrolysis and derivatisation only one validated multi-method for milk analysis is published (Kaufmann et al 2015) and this method is a combination of only the NF metabolites and CAP.…”

Section: Introductionmentioning

confidence: 99%

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A single method to analyse residues from five different classes of prohibited pharmacologically active substances in milk

Bongers

Schans

Nibbeling

et al. 2021

Food Additives & Contaminants: Part A

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In the European Union, the use of veterinary drugs belonging to the A6 group is prohibited in foodproducing animals according to Commission Regulation (EU) No. 2010/37. The aim of this study was to improve the analytical control strategy by developing a single method to analyse residues of prohibited pharmacologically active substances in milk. For this, a single method was developed to analyse 16 prohibited pharmacologically active substances belonging to five different substance classes at required or recommended levels: nitroimidazoles at 3 μg kg −1 , nitrofurans at 0.5 μg kg −1 , chloramphenicol at 0.1 μg kg −1 , dapsone at 5 μg kg −1 and chlorpromazine at 1 μg kg −1 . Milk sample preparation started with an acid hydrolysis combined with a derivatisation. These steps were followed by a clean-up consisting of a dispersive solid-phase extraction and a liquid-liquid extraction. Finally, the sample extracts were analysed by liquid chromatography combined with tandem mass spectrometry, operating alternately in the positive and negative mode. The method was fully validated according to Commission Decision 2002/657/EC for bovine milk and additionally validated for caprine milk. The validation proved that the method is highly effective to detect and confirm all 16 substances in bovine and caprine milk and, additionally to quantify 15 of these substances in bovine milk and 13 of these substances in caprine milk. This study resulted in a new multi-class method to detect, quantify and confirm the identity of 16 prohibited pharmacologically active substances belonging to five different substance classes in two types of milk.

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Section: Introductionmentioning

confidence: 99%