Analysis of nicastrin gene phylogeny and expression in zebrafish

Lim, Anne; Nik, Seyyed Hani Moussavi; Ebrahimie, Esmaeil; Lardelli, Michael

doi:10.1007/s00427-015-0500-9

Cited by 6 publications

(2 citation statements)

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“…Both mapta and maptb have been manipulated to further understand the function and role their ratios play in AD pathogenesis. The use of transgenic zebrafish expressing human MAPT showed biochemical changes consistent with those observed in human tauopathies supporting similarities among zebrafish Mapta and Maptb and human MAPT [ 46 ].…”

Section: The Zebrafish As a Complementary Model For Ad Researchmentioning

confidence: 73%

Use of Zebrafish Genetic Models to Study Etiology of the Amyloid-Beta and Neurofibrillary Tangle Pathways in Alzheimer's Disease

Kiper

Freeman

2022

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: The prevalence of neurodegenerative diseases is increasing globally, with an imperative need to identify and expand the availability of pharmaceutical treatment strategies. Alzheimer's disease is the most common neurodegenerative disease for which there is no cure or has limited treatments. Rodent models are primarily used in Alzheimer's disease research to investigate causes, pathology, molecular mechanisms, and pharmaceutical therapies. However, there is a lack of a comprehensive understanding of Alzheimer's disease causes, pathogenesis, and optimal treatments due in part to some limitations of using rodents, including higher economic cost, which can influence sample size and ultimately statistical power. It is necessary to expand our animal model toolbox to provide alternative strategies in Alzheimer's disease research. The zebrafish application in neurodegenerative disease research and neuropharmacology is greatly expanding due to several vital strengths spanning lower economic costs, the smaller size of the organism, a sequenced characterized genome, and well described anatomical structures. These characteristics are coupled to the conserved molecular function and disease pathways in humans. The existence of orthologs for genes associated with Alzheimer's disease in zebrafish is also confirmed. While wild-type zebrafish appear to lack some of the neuropathological features of Alzheimer's disease, the advent of genetic editing technologies has expanded evaluation of the amyloid and neurofibrillary tangle hypotheses using the zebrafish and exploration of pharmaceutical molecular targets. An overview of how genetic editing technologies are being used with the zebrafish to create models to investigate the causes, pathology, molecular mechanisms, and pharmaceutical targets of Alzheimer's disease is detailed.

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Section: The Zebrafish As a Complementary Model For Ad Researchmentioning

confidence: 73%

Use of Zebrafish Genetic Models to Study Etiology of the Amyloid-Beta and Neurofibrillary Tangle Pathways in Alzheimer's Disease

Kiper

Freeman

2022

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“…Auf transkriptioneller Ebene besteht somit eine Übereinstimmung von 56% mit der ncstn-Gensequenz des Zebrafisches. Diese Homologie zwischen Menschen und Zebrafisch findet sich insbesondere auch bezüglich der funktionell bedeutsamen Genabschnitte Glu333 und der Aminosäuren 312 -340, die für die Substraterkennung und -bindung verantwortlich sind(Lim et al 2015). Auf Grund der zuvor beschriebenen Homologie erschien uns der Zebrafisch als geeignetes Modell, um einige der beim Menschen beschriebenen NCSTN-Mutationen und ihre Effekte in-vivo zu untersuchen, insbesondere in Hinsicht auf möglicherweise kurative molekularbiologische Phänotypreversionsansätze.4.3.1 Kurative Phänotyp-Reversion mitZebrafisch-und humaner RNA In der hier vorgelegten Dissertationsschrift konnten wir zunächst zeigen , dass wir eine durch eine ncstn-MO induzierte Pigmentierungsstörung durch die Behandlung mit einer intakten ncstn-RNA revertieren konnten.…”

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