Analysis of Natural Killer (NK) Cell Subsets Defined by the Expression of Two Novel Surface Antigens (EB6 and GL183) in AIDS and AIDS-Related Conditions

Cauda, Roberto; Goletti, Delia; Lucia, Mothanje Barbara; Tumbarello, Mario; Rumi, Carlo; Orengo, Anna Maria; Moretta, A.

doi:10.1006/clin.1994.1029

Cited by 25 publications

(18 citation statements)

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“…We found decreased expression of KIR receptors on CD56 cells. This has been reported earlier only by Cauda et al [1994], whereas Andre et al [1999] and Galiani et al [1999] reported no signi®cant changes in the expression of these receptors on NK cells in HIVinfected individuals. It is noteworthy that the KIR family comprises many members, which could be activatory or inhibitory with regard to their effect on NK cell functions.…”

Section: Discussionsupporting

confidence: 64%

Modulation of expression of the MHC class I‐binding natural killer cell receptors, and NK activity in relation to viral load in HIV‐infected/AIDS patients

Ahmad

Sindhu

Tran

et al. 2001

Journal of Medical Virology

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The natural killer (NK) cells play an important role in viral infections via their spontaneous cytolytic activity against virus-infected cells as well as via secreting a variety of soluble mediators. The MHC class I-binding NK receptors of these cells have emerged as the most important regulators of the effector activities of cytolytic cells (both NK and CTL). We have studied the modulation of NK activity and the expression of NK receptors in HIV-infected/AIDS patients and report here that the NK activities of the patients with the lowest plasma HIV load were minimal and vice versa, suggesting a decrease in this activity following suppression of HIV replication. Interestingly, the NK activity correlated negatively with the peripheral blood CD4+ T-cell counts of these patients. Furthermore, these patients showed decreased percentages of CD56+ cells expressing NK receptors of the immunoglobulin superfamily, whereas the percentages of CD8+ cells expressing these receptors were increased. Moreover, the expression of C-type lectin-like NK receptor-associated invariant molecule CD94 was increased on CD8+ cells in these patients as compared with HIV-seronegative controls. These changes in the expression of NK receptors were also evident within groups of these patients having different viral loads. These results show, for the first time, decreased innate immunity and changes in the expression of NK receptors on cytolytic cells in relation to viral burden in HIV-infected/AIDS patient.

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Section: Discussionsupporting

confidence: 64%

Modulation of expression of the MHC class I‐binding natural killer cell receptors, and NK activity in relation to viral load in HIV‐infected/AIDS patients

Ahmad

Sindhu

Tran

et al. 2001

Journal of Medical Virology

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“…In addition, recent reports have provided evidence that in chronic viral infections, including HTLV-I and cytomegalovirus, virusspecific cytotoxic T lymphocyte (CTL) responses become progressively oligoclonal (18,19). Interestingly, increased percentages of peripheral blood T cells expressing the HLA-Cspecific NKR p58.2 recently have been detected in HIV-1-infected patients (20). In the present study, we demonstrate that all the presently known inhibitory NKRs can be expressed by T lymphocytes in patients with HIV infection.…”

supporting

confidence: 69%

“…Previous data by Cauda et al (20) indicated that T cells expressing p58.2 frequently were increased in HIV-infected patients. The availability of mAbs to additional inhibitory NKRs, including p58.1, p70, and CD94͞NKG2A, allowed a more precise analysis of NKR ϩ T cells.…”

Section: Resultsmentioning

confidence: 90%

Expression of HLA class I-specific inhibitory natural killer cell receptors in HIV-specific cytolytic T lymphocytes: Impairment of specific cytolytic functions

Maria

Ferraris

Guastella

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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Human T lymphocytes have been shown to express inhibitory natural killer cell receptors (NKR), which can down-regulate T cell antigen receptor-mediated T cell function, including cytolytic activity. In the present study, we demonstrate that CD3 ؉ NKR ؉ cells can be identified in HIVinfected patients. HIV-specific cytolytic activity was analyzed in five patients in whom autologous lymphoblastoid B cell lines could be derived as a source of autologous target cells. Phytohemagglutinin-activated T cell populations that had been cultured in interleukin 2 displayed HIV-specific cytotoxic T lymphocyte (CTL) activity against HIV env, gag, pol, and nef in 3 of 5 patients. Addition of anti-NKR mAb of IgM isotype could increase the specific CTL activity. Moreover, in one additional patient, HIV-specific CTL activity was undetectable; however, after addition of anti-NKR mAb such CTL activity appeared de novo. Similar results were obtained by analysis of CD3 ؉ NKR ؉ clones derived from two patients. These data provide direct evidence that CD3 ؉ NKR ؉ cells may include antigen (HIV)-specific CTLs and that mAb-mediated masking of inhibitory NKR may revert the down-regulation of CTL function.

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“…NK cell number and function are both early affected after HIV infection (14), and related to the progression of the disease (11, [33][34]. Moreover, a specific regulation of CD94 lectin-like NK cell receptors, in comparison with Ig-like NKT cell receptors, was found in HIV-infected individuals (17).…”

Section: Discussionmentioning

confidence: 99%

HLA-E Up-Regulation Induced by HIV Infection May Directly Contribute to CD94-Mediated Impairment of NK Cells

Martini

Agrati

D’Offizi

et al. 2005

Int J Immunopathol Pharmacol

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Alterations in NK cell numbers and function have been repeatedly shown during HIV infection. In this study, NK cell number and MHC class I expression on CD4+ T cells were studied in HIV patients at different stages of disease progression. An increased expression of HLA-E was seen on CD4+ T cells. In parallel, a reduced number of CD94+ NK cells was observed in advanced disease stages. Moreover, a decline in CD94 expression on NK cells was observed at the HIV replication peak in patients undergoing antiretroviral treatment interruption, suggesting a role of viral replication on NK cells alterations. In vitro HIV infection induced a rapid down-regulation of HLA-A,B,C expression, paralleled by an increased expression of HLA-E surface molecules, the formal ligands of CD94 NK receptors. HIV-infected HLA-E expressing cells were able to inhibit NK cell cytotoxicity through HLA-E expression, since cytotoxicity was restored by antibody masking experiments. These data indicate that the CD94/HLA-E interaction may contribute to NK cell dysfunction in HIV infection, suggesting a role of HIV replication in this process.

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Analysis of Natural Killer (NK) Cell Subsets Defined by the Expression of Two Novel Surface Antigens (EB6 and GL183) in AIDS and AIDS-Related Conditions

Cited by 25 publications

References 0 publications

Modulation of expression of the MHC class I‐binding natural killer cell receptors, and NK activity in relation to viral load in HIV‐infected/AIDS patients

Modulation of expression of the MHC class I‐binding natural killer cell receptors, and NK activity in relation to viral load in HIV‐infected/AIDS patients

Expression of HLA class I-specific inhibitory natural killer cell receptors in HIV-specific cytolytic T lymphocytes: Impairment of specific cytolytic functions

HLA-E Up-Regulation Induced by HIV Infection May Directly Contribute to CD94-Mediated Impairment of NK Cells

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