Analysis of N-Acetylglucosamine From Enzymatic Degradationof Amorphous Chitin

Abstract

N-Acetylglucosamine is the monomeric unit of the polymer chitin with (1,4)-β-linkages and the second most abundant carbohydrate after cellulose. The purpose of this study was to analyze N-acetyl glucosamine from enzymatic degradation chitin. The method used is the enzymatic hydrolysis of chitin types amorphous using chitinase from Pseudomonas sp. TNH54. Chitin is a kind of amorphous modified chitin and showed high activity against chitinase. N-acetylglucosamine production optimization has been done with a substrate concentration of 1.2%, enzyme concentration of 0.097 U/mL and the incubation time of 8 hours. The result of purification with ethanol shows a peak with a retention time of 3.1 and a purity of 96.7%. The result showed that the N-acetyl glucosamine hydrolysis enzymatic is in accordance with the standards. Furthermore, experiments to establish purification of N-Acetylglucosamine by ethanol -acetonitrile provides a fairly high yield with similar properties commercial N-Acetylglucosamine

Production, purification and characterization of chitinase from Micromonospora sp. AR17

“…This result is undoubtedly better than crude enzyme, which takes four days to pro duce only 6.59 µg/mL of NAG. However, this result is still much smaller than Herdyastuti and Cahyaningrum (2017) research, chitinase from Pseudomonas sp. TNH54 pro duced Nacetylglucosamine with a concentration of 1,360 µg/mL, a fermentation time of 8 h, and an enzyme concen tration of 0.1 U/ml.…”