Analysis of mycotoxins in barley using ultra high liquid chromatography high resolution mass spectrometry: Comparison of efficiency and efficacy of different extraction procedures

Rubert, Josep; Džuman, Zbyněk; Václavíková, Marta; Zachariášová, Milena; Soler, Carla; Hajšlová, Jana

doi:10.1016/j.talanta.2012.07.010

Cited by 106 publications

(62 citation statements)

References 36 publications

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“…The most comprehensive studies describing the use of HRMS techniques in multimycotoxin analysis were published by Herebian et al [81], Rubert et al [52], De Dominics et al [82], and Beccari et al [53]. These articles cover the main mycotoxin representatives of Fusarium, Claviceps, Aspergillus, Penicillium, and Alternaria fungi and the applicability of TOF and Orbitrap MS systems for both screening and quantitative analysis of the respective toxins in cereal-based matrices.…”

Section: High-resolution (Lc-hrms) Approaches For the Targeted Determmentioning

confidence: 99%

“…It was also concluded that the HRMS technique is fully usable and a time-saving method for rapid and accurate analysis of mycotoxins. LC-Orbitrap MS instrumentation was used for the determination of all legislatively regulated mycotoxins in wheat and barley flours and crisp bread [52]. With the support of the SPE cleanup of extracts, it was possible to quantify all desired mycotoxins, and all validation data met the current European regulatory requirements for LC-MS confirmatory analysis [29].…”

Section: High-resolution (Lc-hrms) Approaches For the Targeted Determmentioning

confidence: 99%

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Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

125

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Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

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Section: High-resolution (Lc-hrms) Approaches For the Targeted Determmentioning

confidence: 99%

Section: High-resolution (Lc-hrms) Approaches For the Targeted Determmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

125

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“…The extraction method was based on an earlier published study [20] and was carried out as follows: 40g slurry (1:1 w/v) or 50g slurry (1:1.5 w/v) were mixed with 60 mL acetonitrile, and the mixture was shaken for 2 h in a horizontal shaker. After filtration, 1 mL of the extract was taken and was diluted in 3 mL of water (in the case of 1:1 w/v slurry) or 2.55 ml (in the case of 1:1.5 w/v slurry).…”

Section: Extractionmentioning

confidence: 99%

“…Most of these methods are based on the combination of high-or ultra-highperformance liquid chromatography with tandem [18][19][20][21] or highresolution [22,23] mass spectrometry. A major advantage of LC-MSbased multi-target methods is the elimination of the need for sample derivatization, in addition with the high selectivity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…However, for fumonisins, higher extraction recoveries are achieved when the water proportion is increased and/or the pH of the solvent is decreased [19]. For the cleanup, multi-target methods use simple "dilute-and-shoot" approaches [19][20]24,25], solid phase extraction [21,26,27], immunoaffinity columns [28,29] and, more recently QuEChERS methodology (Quick, Easy, Cheap, Effective, Rugged and Safe) [23,29]. However, multi-analyte methods with very simple sample preparation are leading to matrix effects which have to be considered for the quantification.…”

Section: Introductionmentioning

confidence: 99%

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A Validated UPLC-MS/MS Multi-mycotoxin Method for Nuts and Cereals for the Official Control in Cyprus within the EU Requirements

Kafouris¹,

Christofidou²,

Christodoulou³

et al. 2015

J Food Process Technol

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The validation of a rapid, reliable and sensitive method for the simultaneous determination of Aflatoxins (AFB 1 , AFB 2 , AFG 1 , AFG 2 ), Ochratoxin A, Zearalenone, Deoxynivalenol, Fumonisins B 1 and B 2 , T-2 and HT-2 toxins, in nuts (peanuts, pistachio and almonds) and cereals (maize and wheat) is reported. The method was developed and validated to fulfil the requirements of the official control of mycotoxins according to EU legislation. This method is based on a single extraction step using an acetonitrile/ water mixture followed by the analysis of the diluted crude extract using ultra high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The MS/MS detection was carried out using an electro spray-ionization interface (ESI) in positive ion mode. Matrix-matched calibration was used for the quantification of the mycotoxins, because of the absence of further clean up steps that reduce suppression/enhancement matrix effects. The method performance characteristics were determined after spiking blank samples on multiple levels. The mean recoveries of mycotoxins in spiked nuts ranged from 74.4% to 131.7%, while in cereals ranged from 52.8% to 113.9%. Relative standard deviations were lower than 20.4% for all target mycotoxins. Limits of detection and quantification for nuts and cereals ranged 0.08-30.0 and 0.25-99.0 μg/Κg, respectively.

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Screening and High‐Throughput Multi‐Contaminants Methods

Kalogiouri

Τhomaidis

2017

Food Authentication

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Analysis of mycotoxins in barley using ultra high liquid chromatography high resolution mass spectrometry: Comparison of efficiency and efficacy of different extraction procedures

Cited by 106 publications

References 36 publications

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

A Validated UPLC-MS/MS Multi-mycotoxin Method for Nuts and Cereals for the Official Control in Cyprus within the EU Requirements

Screening and High‐Throughput Multi‐Contaminants Methods

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