Analysis of mRNA and Long Non-Coding RNA Expression Profiles in Developing Yorkshire Pig Spleens

Li, Xinjian; Han, Xuelei; Sun, Caixia; Gai-ying, LI; Wang, Kejun; Qiao, Ruimin

doi:10.3390/ani11102768

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…Compared to 14 vs. 1 d and 28 vs. 1 d, the number of differential mRNAs and lncRNAs in 28 vs. 14 d is significantly lower. We speculate that the immune system of piglets starts to establish itself before 14 days, which is in general agreement with the view that the immune system of pigs is established at seven days of life (43).…”

Section: Discussionsupporting

confidence: 91%

“…The spleen is one of the pigs' most important immune organs. Additionally, studies have reported many epidemics in pigs to be associated with spleen damage, such as PRRS, Porcine Circovirus, pseudorabies, and swine fever ( 43 ). There are two existing studies on lncRNAs in the spleen of Chinese endemic pigs, and these studies focused on the multiple developmental stages of the pig spleen ( 22 , 43 ).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, studies have reported many epidemics in pigs to be associated with spleen damage, such as PRRS, Porcine Circovirus, pseudorabies, and swine fever ( 43 ). There are two existing studies on lncRNAs in the spleen of Chinese endemic pigs, and these studies focused on the multiple developmental stages of the pig spleen ( 22 , 43 ). In our study, Meishan piglets were selected as a model to analyse the transcriptional expression of lncRNA and mRNA in the spleen tissue of piglets for the first time.…”

Section: Discussionmentioning

confidence: 99%

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Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets

Shi

Xu²,

Wu³

et al. 2022

Front. Vet. Sci.

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Meishan pigs are a famous local pig breed in China, with high fertility and early sexual maturity, and stronger immunity compared to other breeds. The spleen is the largest lymphoid organ in pigs and performs essential functions, such as those relating to immunity and haematopoiesis. The invasion of many pathogenic microorganisms in pigs is associated with spleen damage. Long non-coding RNAs participate in a broad range of biological processes and have been demonstrated to be associated with splenic immune regulation. However, the expression network of mRNAs and lncRNAs in the spleen of Meishan pigs remains unclear. This study collected spleen tissues from Meishan piglets at three different ages as a model, and mRNA and lncRNA transcripts were profiled for each sample. Additionally, 1,806 differential mRNAs and 319 differential lncRNAs were identified. A complicated interaction between mRNAs and lncRNAs was identified via WGCNA, demonstrating that lncRNAs are a crucial regulatory component in mRNA. The results show that the modules black and red have similar mRNA and lncRNA transcription patterns and are mainly involved in the process of the immune defense response. The core genes (DHX58 and IFIT1) and key lncRNAs (TCONS-00002102 and TCONS-00012474) of piglet spleen tissue were screened using the ceRNA network. The expression of these genes is related to the immune response of pigs. Our research may contribute to a further understanding of mRNA and lncRNA expression in the spleen of piglets, and provide new ideas to improve the disease resistance of piglets.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets

Shi

Xu²,

Wu³

et al. 2022

Front. Vet. Sci.

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“…Additionally, FERH regulates signal transduction, intracellular signaling cascade, and glucose and lipid metabolism. rrp7a proteins regulate immune response, the proliferation of blood cells [ 34 ]. ATP10A is a transmembrane protein in the p-type cation transport ATPase family [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

The Immunoprotection of OmpH Gene Deletion Mutation of Pasteurella multocida on Hemorrhagic Sepsis in Qinghai Yak

Jia

Zhao

et al. 2023

Veterinary Sciences

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OmpH is among the most important virulence factors of Pasteurella multocida, which mediates septicemia in yaks (Bos grunniens I) after infection with the bacteria. In the present study, yaks were infected with wild-type (WT) (P0910) and OmpH-deficient (ΔOmpH) P. multocida strains. The mutant strain was generated through the reverse genetic operation system of pathogens and proteomics technology. The live-cell bacterial count and clinical manifestations of P. multocida infection in Qinghai yak tissues (thymus, lung, spleen, lymph node, liver, kidney, and heart) were analyzed. The expression of differential proteins in the yak spleen under different treatments was analyzed using the marker-free method. We found that compared with the mutant strain, the titer of wild-type strains was significantly higher in tissues. Additionally, compared with other organs, the bacteria titer was significantly higher in the spleen. Compared with the WT p0910 strain, the mutant strain generated milder pathological changes in the tissues of yak. Proteomics analysis revealed that 57 of the 773 proteins expressed in P. multocida were significantly differentially expressed between the ΔOmpH and P0910 groups. Of the 57, 14 were over-expressed, whereas 43 were under-expressed. The differentially expressed proteins in the ΔompH group regulated the ABC transporter (ATP-powered translocation of many substrates across membranes) system, the two-component system, RNA degradation, RNA transcription, glycolysis/gluconeogenesis, biosynthesis of ubiquinone and other terpenoid-quinones, oxidative phosphorylation (citrate cycle) as well as fructose and mannose metabolism. The relationship among 54 significantly regulated proteins was analyzed using STRING. We found that WT P0910 and ΔOmpH of P. multocida infection activated the expression of ropE, HSPBP1, FERH, ATP10A, ABCA13, RRP7A, IL-10, IFN-γ, IL-17A, EGFR, and dnaJ. Overall, deletion of the OmpH gene weakened the virulence but maintained the immunogenicity of P. multocida in yak. The findings of this study provide a strong foundation for the pathogenesis of P. multocida and the management of related septicemia in yaks.

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“…Another study has established that LncRNA-Cox2 regulates macrophage polarization and inflammatory responses in septic mice via the CREB-C/EBP signaling pathway [ 10 ]. In addition, Qiao et al [ 11 ] have provided transcriptome profiles of mRNAs and lncRNAs at development stages in Yorkshire pigs. However, no studies have explored lncRNA-mRNA interactions during the spleen developmental stages of Meishan pigs.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-Wide lncRNA and mRNA Profiling of Spleens from Meishan Pigs at Different Development Stages

Shi

Huang

et al. 2022

Animals

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Meishan is a well-established local Chinese breed known for its high fecundity, strong immune response and high meat quality. However, the molecular mechanism of immune regulation during the development of Meishan pigs still remains unclear. Here, we performed the transcriptional sequencing of spleen tissues from Meishan pigs at different development stages. In total, 10,268 lncRNAs were identified, including 1254 novel lncRNAs and 9014 known lncRNAs. Time series analysis revealed that genes of the up-regulated module were enriched in pathways associated with transport, immunity, and histone acetylation modifications, while genes of the down-regulated module were enriched in DNA metabolic process and cell cycle. Weighted gene co-expression network analysis (WGCNA) showed the functional linkage between mRNAs and lncRNAs, indicating that lncRNAs are important regulatory elements of mRNAs. Notably, a lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network that contained 3 mRNAs (AKT3, CBL and PTK2B), 17 lncRNAs and 67 miRNAs were screened out, which probably plays a critical role in immune regulation of Meishan pigs. Our findings not only revealed the transcriptome profile of spleen development, but also provide novel insights into the mechanism of lncRNA-miRNA-mRNA axis in the immune response in Meishan pigs.

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Analysis of mRNA and Long Non-Coding RNA Expression Profiles in Developing Yorkshire Pig Spleens

Cited by 5 publications

References 22 publications

Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets

Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets

The Immunoprotection of OmpH Gene Deletion Mutation of Pasteurella multocida on Hemorrhagic Sepsis in Qinghai Yak

Transcriptome-Wide lncRNA and mRNA Profiling of Spleens from Meishan Pigs at Different Development Stages

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