Analysis of morphological characteristics and expression levels of extracellular matrix proteins in skin wounds to determine wound age in living subjects in forensic medicine

Fronczek, Judith; Lulf, Ronald; Korkmaz, Halil İbrahim; Witte, Birgit I.; Goot, Franklin R.W. van de; Begieneman, Mark P.V.; Krijnen, Paul A.J.; Rozendaal, Lawrence; Niessen, Hans W.M.; Reijnders, Udo

doi:10.1016/j.forsciint.2014.11.013

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…The three proinflammatory cytokines proved to be useful markers for determining a lesion onset interval of up to a few hours. The first increase in reactivity could be noted for IL-1β, IL-6, and TNF-α, almost simultaneously after 15 ± 20 min, their expression changing after ∼1 ± 1.5 h. (31,44,45,53,62) were excluded because they do not place the appearance and/or quantification of a marker on a timeline about the age of the wound.…”

Section: Results Fit With Other Available Datamentioning

confidence: 99%

Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review

et al. 2022

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Background: In forensic autopsies, examining the wounds is one of the most critical aspects to clarify the causal relationship between the cause of death and the wounds observed on the corpse. However, on many occasions, it is difficult to differentiate antemortem injuries from post-mortem injuries, mainly when they occur very close to the moment of death. At present, various studies try to find biomarkers and clarify the molecular mechanisms involved in a wound due to the high variability of conditions in which they occur, thus being one of the most challenging problems in forensic pathology. This review aimed to study the omics data to determine the main lines of investigation emerging in the diagnosis of vital injuries, time of appearance, estimation of the age and vitality of the wound, and its possible contributions to the forensic field.Methods: A systematic review of the human wound concerning forensic science was carried out by following PRISMA guidelines.Results: This study sheds light on the role of omics research during the process of wounding, identifying different cytokines and other inflammatory mediators, as well as cells involved in the specific stage of the wound healing process, show great use in estimating the age of a wound. On the other hand, the expression levels of skin enzymes, proteins, metal ions, and other biomarkers play an essential role in differentiating vital and post-mortem wounds. More recent studies have begun to analyze and quantify mRNA from different genes that encode proteins that participate in the inflammation phase of a wound and miRNAs related to various cellular processes.Conclusions: This study sheds light on the role of research in the molecular characterization of vital wounds, heralding a promising future for molecular characterization of wounds in the field of forensic pathology, opening up an important new area of research.Systematic Review Registration: URL: https://www.crd.york.ac.uk/prospero/#myprospero, Identifier: CRD42021286623.

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Section: Results Fit With Other Available Datamentioning

confidence: 99%

Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review

et al. 2022

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“…Ethical issues related to the use of tissue from living donors are also important. Indeed, the use of humans in research is subject to approval by the local ethics committee [8,9]. Moreover, as samples from injuries sustained by live human subjects must be obtained in a non-invasive manner (swabbing), so the sample size is frequently inadequate, leading to unreliable, sometimes false-negative results [8,9,15].…”

Section: Common Tissues Used To Date Woundsmentioning

confidence: 99%

“…Another obstacle is the availability of, and the not negligible ethical issues involved with, using human specimens with a known time of death [7][8][9]. Therefore, animal studies are essential, but the applicability of the results to humans lacks definitive supporting evidence.…”

Section: Introductionmentioning

confidence: 99%

Vitality and wound-age estimation in forensic pathology: review and future prospects

Bai

et al. 2018

Forensic Sciences Research

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Determining the age of a wound is challenging in forensic pathology, but it can contribute to the reconstruction of crime scenes and lead to arrest of suspects. Forensic scholars have tended to focus on evaluating wound vitality and determining the time elapsed since the wound was sustained. Recent progress in forensic techniques, particularly high-throughput analyses, has enabled evaluation of materials at the cellular and molecular levels, as well as simultaneous assessment of multiple markers. This paper provides an update on wound-age estimation in forensic pathology, summarizes the recent literature, and considers useful additional information provided by each marker. Finally, the future prospects for estimating wound age in forensic practise are discussed with the hope of providing something useful for further study.

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“…Fronczek ve ark. yaşayan kişilerden yara bölgesinden alınan yüzeyel deri biopsi materyallerinde inflamatuar hücreleri (MPO, CD45, CD68) ve inflamatuar mediatörleri (MIP-1, IL-8, CML, vitronektin) değerlendirerek yara yaşı değerlen-dirmesinde kullanılabilecek bir skorlama sistemi öner-mişlerdir (37,38). Yagi ve ark (2016) ise transmembran proteini olan ve inflamatuar hücrelerden sentezlenen CD14'ün erken dönem yara iyileşmesinde (1-5) etkin olduğu, en yüksek seviyeyi 12-24 saatte yaptığını saptamıştır (39).Van de Goot ve ark ise (2014) erken dö-nem markerlardan olan fibronektin, CD62p ve faktör 8'i immunhistokimyasal yöntemle belirleyip bir skorlama sistemi oluşturmuştur.…”

Section: Lokal Faktörlerunclassified

Yara Yaşı ve Canlılık Değerlendirmesinde Güncel Yaklaşım

Pakiş

2016

Bull Leg Med

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Adli tıp uygulamalarının ana konularından biri olan yaralar, kişiler canlı iken, ölüm sırasında ve ölüm sonrası gelişebilmektedir. Yaralanmanın kişi canlı iken mi, öldükten sonra mı oluştuğu adli tıbbın önemli sorunlarından biridir. Kesici ya da künt travmalar, yanıklar, boğulmalar gibi travmatik olgularda canlılığın gösterilmesi travma ile ilişki sağlar. Canlılık kanıtlanırsa bu travmanın kişi yaşarken olduğunun kanıtıdır (1,2).Travma ile ölüm arasındaki geçen sürenin belirlenmesi yani yara yaşı tayini de adli tıbbın temel çalışma alanlarındandır. Bu süre dakikalardan aylara hatta yıllara uzayabilmektedir. Çok erken dönemlerde yara yaşı tayini canlılıkla eş değerdir. Travmadan sonraki ilk 30 dakikadaki değişimler hem canlılık bulgusu, hem de yara iyileşmesinin işaretleri olarak kabul edilebilir. En zor soru ÖzetYara yaşı ve canlılık değerlendirilmesi adli tıbbın temel alanlarından biridir. Yaralanmanın kişi canlı iken mi, öldük-ten sonra mı oluştuğu adli tıbbın önemli sorunlarından biridir. Kesici ya da künt travmalar, yanıklar, boğulmalar gibi travmatik olgularda travma ile ilişkiyi sağlar. Canlılık kanıt-lanırsa bu travmanın kişi yaşarken olduğunun kanıtıdır. Literatürde yara yaşı tahmini ve canlılık ile ilgili yapılmış çok sayıda araştırma bulunsa da yapılacak çok sayıda araştırmaya ve araştırmalardan elde edilen bilginin günlük uygulamalara aktarılmasına ihtiyaç vardır. Çalışmada amaç yara yaşı ve canlılık değerlendirmesinde güncel uygulamalar ışığında kullanılan yöntem ve maddeleri adli tıp pratiği açısından değerlendirmektir.Anahtar Kelimeler: Yara yaşı; Canlılık; Adli patoloji. AbstractResearch on vitality and wound age estimation are the classic fields in forensic medicine. Vitality as one of the basic issues in forensic practice deals with the question, whether injuries were caused during lifetime of an individual. It plays a role in connection with traumatic deaths due to sharp and blunt force injuries, burns or drowning. In relation to the vitality of wounds it must be asserted that an injury of the body was caused during lifetime. In spite of large literature data there is still considerable demand of further research and practical transfer of knowledge and techniques to daily casework. The study aims to classify current methods and materials used in evaluation of wound age and viability and determine their practical application in forensic medicine .

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Analysis of morphological characteristics and expression levels of extracellular matrix proteins in skin wounds to determine wound age in living subjects in forensic medicine

Cited by 8 publications

References 25 publications

Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review

Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review

Vitality and wound-age estimation in forensic pathology: review and future prospects

Yara Yaşı ve Canlılık Değerlendirmesinde Güncel Yaklaşım

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