Analysis of monoclonal antibodies that recognize γδ T/null cells

Davis, William C.; Zuckermann, Federico A.; Hamilton, Mary Jo; Barbosa, J I Rodríguez; Saalmüller, Armin; Binns, R. M.; Licence, S. T.

doi:10.1016/s0165-2427(97)00107-4

Cited by 62 publications

(38 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MAb immunoprecipitating heterodimers of 37 and 40 kDa MW [60, 61,242] recognised a constant region of the -chain [61] and first demonstrated that the majority of 'null cells' (CD2 − sIg − ) are TcR-T cells [23,243]. Some other mAb recognise different epitopes of the -chain [61], whereas 7G3 was demonstrated suitable for high-quality immunostaining on frozen sections [218].…”

Section: Tcr and -Chainmentioning

confidence: 99%

“…Some other mAb recognise different epitopes of the -chain [61], whereas 7G3 was demonstrated suitable for high-quality immunostaining on frozen sections [218]. In addition, PG83A and 86D specifically recognise the porcine -chain of the TcR [61].…”

Section: Tcr and -Chainmentioning

confidence: 99%

“…The anti-bovine WC1 mAb CC101 recognises a conserved determinant of WC1 of sheep and cattle and cross-reacts with porcine lymphocytes [24,61]. While WC1 is expressed on the majority of ruminant T cells only the CD2…”

Section: Anti-wc1mentioning

confidence: 99%

See 2 more Smart Citations

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

View full text Add to dashboard Cite

-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

show abstract

Section: Tcr and -Chainmentioning

confidence: 99%

Section: Tcr and -Chainmentioning

confidence: 99%

See 1 more Smart Citation

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

View full text Add to dashboard Cite

show abstract

“…This is largely due to the lack of mAbs which recognize V␦ or V␥ chains. In addition, there is still a lack of an Ab which recognizes all ␥␦ T cells (19,20). Thus, currently the analysis is largely restricted to molecular biological methods.…”

mentioning

confidence: 99%

Development and Compartmentalization of the Porcine TCR δ Repertoire at Mucosal and Extraintestinal Sites: The Pig as a Model for Analyzing the Effects of Age and Microbial Factors

Holtmeier

Käller

Geisel

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

γδ T cells are an important component of the mucosal immune system. Previously, we have shown that the TCR δ repertoire in human intestine is polyclonal at birth and becomes increasingly restricted with age. In this study, we expand those studies to the pig which allows more extensive experiments including several organs. Tissues from different mucosal sites like the stomach, duodenum, ileum, Peyer’s patches, jejunum, and colon, and also extraintestinal sites like the lung, spleen, thymus and mesenteric lymph nodes, were obtained from conventionally reared pigs aged 2 wk to 5.5 years. In addition, tissues were also obtained from 10-wk-old specified pathogen- and germ-free pigs. TCRDV1-DV5 transcripts were amplified by RT-PCR after which complementarity-determining region 3 spectratyping was performed. Individual bands were excised from the gels and directly sequenced. The intestinal TCR δ repertoire showed increasing restriction with age and was highly oligoclonal in the adult 2- to 5.5-year-old pigs. In old pigs, we observed a striking compartmentalization. Different TCR δ repertoires were present between the lungs and the intestinal mucosa but also within different parts of the gastrointestinal tract. However, occasionally we observed identical TCR δ transcripts in the intestine and the lungs and shared clones could be detected also along the entire gastrointestinal tract. Thus, subsets of γδ T cells are likely to transport immunological information between different compartments of the immune system. Furthermore, these data support the hypothesis that in each mucosal site, different Ags are responsible for selecting and maintaining the γδ TCR over time.

show abstract

“…Pigs have a higher population of T cells expressing neither CD4 nor CD8 (CD4-CD8-T cells) compared to other mammals (Davis et al, 1998;Yang and Parkhouse, 2000). This population expresses the yô T-cell receptor (TCR), which differs from the first three subpopulations that express the a|3 TCR.…”

Section: Cd4+cd8-t Lymphocytes Can Further Differentiate Into 2 Diffementioning

confidence: 99%

Investigation of immune response and protection induced by a combined vaccination of modified live and killed porcine reproductive and respiratory syndrome virus vaccines and its potential use in a swine herd

Nilubol

View full text Add to dashboard Cite

Analysis of monoclonal antibodies that recognize γδ T/null cells

Cited by 62 publications

References 17 publications

Membrane markers of the immune cells in swine: an update

Membrane markers of the immune cells in swine: an update

Development and Compartmentalization of the Porcine TCR δ Repertoire at Mucosal and Extraintestinal Sites: The Pig as a Model for Analyzing the Effects of Age and Microbial Factors

Investigation of immune response and protection induced by a combined vaccination of modified live and killed porcine reproductive and respiratory syndrome virus vaccines and its potential use in a swine herd

Contact Info

Product

Resources

About