Analysis of mono‐, di‐, and triglycerides by capillary supercritical fluid chromatography

Abstract

SummaryThe separation of mixed mono-, di-, and triglycerides by capillary supercritical fluid chromatography is described. The separations were performed at low operating temperatures, using a carbon dioxide mobile phase, a conventional flame ionization detector, and two different stationary phases, DB-5 (95% dimethyl-(5%)-diphenylpolysiloxane) and DB-225 (50% cyanopropyl-methyl-(5O0/~)-methylphenylpolysiloxane). Because the separations were performed at low operating temperatures, no thermal degradation of the glycerides wasobserved. Even under these mild operating conditions, trinewonin, a triglyceride having a molecular weight over 11 00 amu, was readily eluted. When rapid pressure programming of the carbon dioxide mobile phase was employed, trinervonin was eluted in less than two minutes. On DB-5, the mono-, di-, and triglycerides eluted in order of increasing molecular weight. A graph of t i (on DB-5) versus molecular weight is linear. When DB-225 was used as the stationary phase, triglycerides eluted in the order of increasing unsaturation-Thus, theorder of elution on 138-225 was tristearin, triolein, trilinolein, and trilinolenin.

Supercritical fluid chromatographic analysis of new crop seed oils and their reactions

“…The percentage of triepoxy TG (54.4%) we obtained is slightly higher than that measured by HPLC (18,24) and than that predicted theoretically with use of a random distribution: 41.9%, based on the oil. Similar SFC calibration curves also have been displayed elsewhere (5,28,29). The slope of the calibration curve is initially steep; then it gradually decreases.…”

“…When open tubular columns with nonpolar stationary phases are employed, separation of glycerides occurs via molecular weight (MW), or equivalently, carbon number, but not by degree of unsaturation (1,(4)(5)(6)(7)(8). Determination of an analyte's MW can be determined simply by creation of a retention time-MW calibration curve (5,6). A recent example demonstrated the ability of SFC to elute triglycerides (TG) with MW over 1500 Da.…”

Triacylglycerol profiling by using chromatographic techniques

“…Stationary phases widely differing in polarity, i.e. crosslinked DB-5 and DB-225, were compared for their applicability to capillary SFC of TGs [217]. On DB-5 TGs with the same CN, i.e.…”

Applications of Supercritical Fluids in Industrial Analysis

“…A preliminary study (White and Houck, 1985) investigated the choice of column for the analysis of mono-, diand triglycerides using FID. The two different stationary phases investigated were DB-5 (95% dimethyl-(5%) diphenylpolysiloxane) and DB-225 (50% cyanopropylmethyl (50%) methylphenylpolysiloxane).…”