Analysis of molecular variation in porcine reproductive and respiratory syndrome virus in China between 2007 and 2012

Abstract

In the present study, 89 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in China during 2007 to 2012 were randomly selected from the GenBank genetic sequence database. Evolutionary characteristics of these isolates were analyzed based on the sequences of non-structural protein 2 (Nsp2) and glycoprotein 5 (GP5). The genetic variations of the isolates were also compared with six representative strains. The results showed that a high degree of genetic diversity exists among the PRRSV population in China. Highly pathogenic PRRSV isolates, with a discontinuous deletion of a 30 amino acid residue in the Nsp2 region, remained the most dominant virus throughout 2007-2012 in China. Owing to the extensive use of representative vaccine strains, natural recombination events occurred between strains. Three isolates - HH08, DY, and YN-2011 - were more closely related to vaccine strains than the other isolates. Both YN-2011 and DY were the evolutionary products of recombination events between strains SP and CH-1R. The results of the present study provide useful information for the epidemiology of PRRSV as well as for vaccine development.

Variations in the NSP4 gene of the type 2 porcine reproductive and respiratory syndrome virus isolated in China from 1996 to 2021

“…Li et al [ 32 ] found that HNyc15 was formed by recombining VR2332 and CH-1a gene fragments. Widespread use of representative attenuated vaccine strains of PRRSV will also increase the diversity of PRRSV in this area [ 33 ]. These genetic events indicate that gene recombination plays a critical role in the evolution of PRRSV.…”

Co-Infection of Swine with Porcine Circovirus Type 2 and Other Swine Viruses

“…Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent that causes porcine reproductive and respiratory syndrome (PRRS), which has been acknowledged as one of the most economically devastating diseases in the swine industry [54,55]. Typical clinical symptoms of PRRS are characterized as blue-ear in the infected pigs, with mild to severe respiratory disease in newborn/weaned piglets and growing pigs, as well as reproductive failure in pregnant pigs [55].…”

Preliminary Study on Prevalence, Risk Factor and Genetic Homogeneity of Porcine Reproductive and Respiratory Syndrome Virus in Registered Pig Farms in Heilongjiang, China

“…Both of the two phylogenetic trees indicated the existence of the two genetically small groups of PRRSVs in Heilongjiang Province, viruses in one of them are more closely related to the strain JXA1 (isolated in southern China), while those in another group are similar to the Chinese isolate Henan‐1 (isolated in central China). The results again proved the Chinese HP‐PRRSVs have increased rapidly in their genetic diversity (Liu et al., ; Cao et al., ).…”

“…Porcine reproductive and respiratory syndrome has posed a great threat to the Chinese pig industry since its emergence in 1995. In the previous studies, the epidemiology, aetiology and genetic diversity of the disease have been extensively studied in east, central and southern China (An et al., , ; Tian et al., ; Ferrari et al., ; Yu et al., ; Yun and Lee, ; Cao et al., ; Wang et al., ; Xie et al., ). However, there was no published information available on the disease in Heilongjiang.…”

“…A mutant PRRSV was later identified to be the causative agent of this disease and was called highly pathogenic PRRSV (HP‐PRRSV) (Tian et al., ; An et al., ). In more recent years, epidemiological and evolutionary studies showed that the circulating PRRSVs were still dominantly HP‐PRRSVs, but the genetic diversity of PRRSV was continuously increasing (Cao et al., ; Xie et al., ).…”