Analysis of mixtures by collisional activation spectrometry: Pyrolysis products of deoxyribonucleic acid

Abstract

Six major components of a mixture of the pyrolysis products of deoxyribonucleic acid have been analysed by combined low energy electron impact and collisional activation spectra. The method allowed the assignment of the structures of the different components in the complex mixture without prior separation.

Historical and contemporary stable isotope tracer approaches to studying mammalian protein metabolism

“…SRM is typically performed using triple quadrupole MS instruments (however can also be performed using other tandem MS configurations such as QTOF), whereby three quadrupole are aligned in series prior to the detector. Following ionisation of the compound in the ion source, the first quadrupole acts as a mass filter allowing passage of a predefined precursor ion to the second quadrupole which is in fact a collision cell containing an inert gas (e.g., argon), where the precursor ion is further fragmented before entering the final quadrupole which acts as another mass filter to select a predefined fragment ion (Levesen & Schulten, ; Kondrat & Cooks, ). This high level of selectivity provided by this technique significantly increases the signal to noise ratio by removing any background or interfering signals from the sample, thereby increasing the sensitivity of the equipment when analysing compounds within complex matrices such as biological samples (Kondrat & Cooks, ; Addona et al, ).…”

Identification of Protected Deoxyribonucleotides by Field Desorption Mass Spectrometry in Fractions from High Performance Liquid Chromatography

“…The ion at mjz 128 indicates the pchlorophenyl moiety of the phosphate triester and the fragment at m/z 98 corresponds by its nominal mass to a degradation product of deoxyribose found in the high resolution FD spectra of DNA [15]. Its structure had been identified by collisional activation mass spectrometry as a mixture of a-angelica lactone and furfuryl alcohol [16].…”

Rapid analysis of complex mixtures by mass spectrometry/mass spectrometry

“…Commercial electrothermal graphite tube atomizers have two limitations: they give relatively low sensitivities because of their relatively low rates of heating; their heating is nonisothermal in time and along the length of the graphite tube, resulting in condensation of the analyte vapor at the cooler ends of the graphite tube and its subsequent re-evaporation producing unpredictable consequences (1)(2)(3)(4)(5)(6)(7)(8)(9). The above two limitations can be removed by using much faster heating rates with a capacitive discharge power supply together with an auxiliary power supply and anisotropic pyrolytic graphite tubes.…”

“…This ability to specifically analyze for particular reactions has led to the development of the mass-analyzed ion kinetic energy spectrometer (MIKES) which records all the reactions of a particular ion in a single scan.This capability has been successfully applied to the direct analysis of specific components in complex mixtures (3, 4). Implicit in the use of a mass analyzer as a separator in mass spectrometry/mass spectrometry (MS/MS) (5)(6)(7)(8)(9)(10)(11) is a time advantage over chromatographic alternatives, This paper seeks to demonstrate this feature explicitly.Speed of analysis is a particularly important consideration in the detection of trace amounts of carcinogens, pollutants, or impurities in complex mixtures where both high sample throughput and high sensitivity are required. The time required for analysis must include that used in sample preparation, viz., isolation, derivat.ization, and purification, as well as the requisite instrumental analysis time.…”

“…This capability has been successfully applied to the direct analysis of specific components in complex mixtures (3, 4). Implicit in the use of a mass analyzer as a separator in mass spectrometry/mass spectrometry (MS/MS) (5)(6)(7)(8)(9)(10)(11) is a time advantage over chromatographic alternatives, This paper seeks to demonstrate this feature explicitly.…”