Analysis of mixed DNA profiles from the RapidHIT™ ID platform using probabilistic genotyping software STRmix™

Ward, Denise; Henry, Julianne; Taylor, Duncan

doi:10.1016/j.fsigen.2022.102664

Cited by 9 publications

(6 citation statements)

References 29 publications

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“…Additionally, rapid DNA techniques are not or less suitable for resolving DNA mixtures [ 5 ]. This results in a trade-off between performance on one hand and speed and portability on the other [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

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A Systematic Review on Commercially Available Integrated Systems for Forensic DNA Analysis

Bruijns

Knotter

Tiggelaar

2023

Sensors

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This systematic review describes and discusses three commercially available integrated systems for forensic DNA analysis, i.e., ParaDNA, RapidHIT, and ANDE. A variety of aspects, such as performance, time-to-result, ease-of-use, portability, and costs (per analysis run) of these three (modified) rapid DNA analysis systems, are considered. Despite their advantages and developmental progress, major steps still have to be made before rapid systems can be broadly applied at crime scenes for full DNA profiling. Aspects in particular that need (further) improvement are portability, performance, the possibility to analyze a (wider) variety of (complex) forensic samples, and (cartridge) costs. Moreover, steps forward regarding ease-of-use and time-to-result will benefit the broader use of commercial rapid DNA systems. In fact, it would be a profit if rapid DNA systems could be used for full DNA profile generation as well as indicative analyses that can give direction to forensic investigators which will speed up investigations.

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Section: Discussionmentioning

confidence: 99%

“…The sensitivity of the RapidHIT is lower than a standard laboratory workflow, which affects the results when touch samples are analyzed. Many of the analyzed touch DNA samples produced very low-level DNA profiles [ 76 ].…”

Section: Rapid Dna Systemsmentioning

confidence: 99%

A Systematic Review on Commercially Available Integrated Systems for Forensic DNA Analysis

Bruijns

Knotter

Tiggelaar

2023

Sensors

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“…These Rapid PCR programs are valuable to forensic investigations as they allow informative DNA profiles to be generated quickly, in the field at remote locations, and by personnel with little training. However, it is important to highlight that Rapid DNA has a specific range of applications, and currently has only had varying success with the suboptimal samples that are typically encountered in casework [95]. Thus, a major limitation of Rapid DNA cycling conditions is that they generally require high-quality samples (i.e., reference samples) to generate good-quality, informative DNA profiles [96].…”

Section: Short Tandem Repeat Profilingmentioning

confidence: 99%

“…The largest alteration to PCR cycling conditions in forensic science came with the introduction of Rapid DNA instruments to laboratory workflows. While the features and importance of Rapid DNA have already been discussed, it is important to emphasize that Rapid DNA was designed for high-quality samples (i.e., reference samples) and has only had varying success when used with sub-optimal samples [95]. This highlights an important limitation of Rapid DNA: it requires good-quality DNA samples to generate informative DNA profiles [96].…”

Section: Evolution Of Pcr Cycling Conditions In Other Disciplinesmentioning

confidence: 99%

PCR in Forensic Science: A Critical Review

McDonald,

Taylor,

Linacre

2024

Genes

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The polymerase chain reaction (PCR) has played a fundamental role in our understanding of the world, and has applications across a broad range of disciplines. The introduction of PCR into forensic science marked the beginning of a new era of DNA profiling. This era has pushed PCR to its limits and allowed genetic data to be generated from trace DNA. Trace samples contain very small amounts of degraded DNA associated with inhibitory compounds and ions. Despite significant development in the PCR process since it was first introduced, the challenges of profiling inhibited and degraded samples remain. This review examines the evolution of the PCR from its inception in the 1980s, through to its current application in forensic science. The driving factors behind PCR evolution for DNA profiling are discussed along with a critical comparison of cycling conditions used in commercial PCR kits. Newer PCR methods that are currently used in forensic practice and beyond are examined, and possible future directions of PCR for DNA profiling are evaluated.

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“…Some attempts to extend the range of sample types to low quantities of DNA or mixtures have been published with various levels of success (see Table 4 ). Researcher and practitioners from Australia [ [40] , [41] , [42] ], Canada [ 43 ], China [ 44 ], Italy [ 45 ], Japan [ 46 , 47 ], and the United States [ [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] ] have contributed to an increased understanding of rapid DNA testing capabilities and limitations.…”

Section: Advancements In Current Practicesmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Analysis of mixed DNA profiles from the RapidHIT™ ID platform using probabilistic genotyping software STRmix™

Cited by 9 publications

References 29 publications

A Systematic Review on Commercially Available Integrated Systems for Forensic DNA Analysis

A Systematic Review on Commercially Available Integrated Systems for Forensic DNA Analysis

PCR in Forensic Science: A Critical Review

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

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