Analysis of Microsatellite Loci of the Chloroplast Genome in the Genus Capsicum (Pepper)

Genetic Relationships Within and Between Capsicum Species

“…Numbers on branches are bootstrap frequency values for 2,000 replicates. C B , C. baccatum complex; C P , C. pubescens complex; C A , C. annuum complex, according to Tong and Bosland (1999), Walsh and Hoot (2001), Jarret and Dang (2004), and Ryzhova and Kochieva (2004) Fig . 3 Differentiation of 15 Capsicum accessions along the first two principal coordinate axes.…”

“…The first axis (PCO 1 ) explained 27.5% of the variance, and the second axis (PCO 2 ) 17.1%. C B , C. baccatum complex; C P , C. pubescens complex; C A , C. annuum complex, according to Tong and Bosland (1999), Walsh and Hoot (2001), Jarret and Dang (2004), and Ryzhova and Kochieva (2004) many plant and animal species for DNA fingerprinting and mapping studies (Pang et al 2009;Rahman et al 2009). In this study, we used a touch-down PCR procedure to enhance repeatability of RAPD banding patterns.…”

“…Various molecular markers including RAPD markers have been used in a variety of plant species (Rodriguez et al 1999;Ryzhova and Kochieva 2004;Ince et al 2009). Although reproducibility of RAPD is open to debate, RAPD markers are used in Fig.…”

“…Modest uncertainty still exists, but the number of species in this genus is approximately 27, consisting of 5 domesticated and 22 wild species (Rodriguez et al 1999;Onus and Pickersgill 2004;Ince et al 2009). Species identification in Capsicum is traditionally based on morphological characteristics and hybridization studies (Hunziker 1950(Hunziker , 1956(Hunziker , 1998Heiser and Smith 1953; RAPD markers have been widely used to quantify genetic variation and mapping studies in closely related plant species, including Capsicum (Ryzhova and Kochieva 2004), Brassica (Jain et al 1994), petunia (Cerny et al 1996), and bermudagrass (Karaca et al 2002). RAPD markers are produced when the primer anneals to template DNA in opposite orientations at intervals usually shorter than 4,000 bp.…”

Nuevas herramientas en la lucha contra las virosis del pimiento

“…C. eximium and C. cardenasii are morphological distinct, but they form natural hybrids that are more fertile than some crosses between varieties of the same species (Eshbaugh 1976;McLeod et al 1979b). In our analysis, these two wild species were indistinguishable from each other using molecular data, as they were clustered together, as was also previously reported (Choong 1998;Ryzhova and Kochieva 2004). This result supports the definition of C. eximium and C. cardenasii as a single, morphologically variable species that belongs to the C. pubescens complex.…”