Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells

Bukhari, Syed I. A.; Truesdell, Samuel S.; Vasudevan, Shobha

doi:10.1007/978-1-4939-7371-2_18

Cited by 5 publications

(3 citation statements)

References 63 publications

(72 reference statements)

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“…Протеїн 1 синдрому фрагільної хромосоми X і затримки розумового розвитку (fragile X mental retardation 1 -FMR1) залежно від етапу клітинного циклу може брати участь як в інгібуванні, так і в посиленні трансляції [1,13]. Протеїни FMR ссавців у процесі РНКінтерференції безпосередньо взаємодіють з протеїнами AGO комплексу RISC [2,3]. Показано, що протеїн FMR1 має здатність розпізнавати декілька кодуючих і некодуючих РНК, взаємодіяти з декількома цитоплазматичними і ядерними протеїнами, включно з AGO2, впливаючи на активність трансляції.…”

Section: мікрорнк-опосередкована активація трансляції що асоційована ...unclassified

Mechanisms of action of cytoplasmic microRNAs. Part 6. MicroRNA-mediated translation activation

Абатуров¹,

Babуch²

2023

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In the scientific review, the mechanisms of action of cytoplasmic miRNAs, namely miRNA-mediated activation of translation, are given. To write the article, information was searched using Scopus, Web of Science, MedLine, PubMed, Google Scholar, EMBASE, Global Health, The Cochrane Library databases. Examples of direct activation of mRNA translation by miRNA are presented. One of them is miRNA-mediated activation of translation, which is associated with the peculiarities of the state of the cell (resting cell effect). It has been shown that protein 1 of the fragile X mental retardation (FMR1) syndrome, depending on the stage of the cell cycle, can participate in both inhibition and enhancement of translation. It is known that microRNAs can influence the activity of RNP by binding to the RNA-binding sites of specific mRNAs or directly to RBP molecules, directly inhibiting their activity. Poly (rC) binding protein 2 (PCBP2) is a multifunctional adapter molecule that binds to RNA and DNA, competing with other RNA-binding factors. The PCBP2 protein limits translation initiation by preventing ribosome recruitment. The authors provided information on miR-346-mediated activation of the translation of receptor-interacting protein 140. It is emphasized that some miRNAs, preventing the degradation of the mRNA molecule, increasе the level of its stability, which is accompanied by an enhancement in their translation. MicroRNAs stabilize specific mRNA targets, preventing the association of the ARE element degradation factor, tristetraprolin, with mRNA. Data are presented on the activation of mRNA target translation by factors that sequester miRNAs or compete with miRNAs. Various intracellular factors and proteins can enter into a competitive relationship with miRNA and interfere with or remove it from the target mRNA. It is known that activation of translation can occur due to microRNA inhibition of repressor proteins. The authors indicate that increased miR-145 expression is accompanied by activation of myocardin translation, which induces the proliferation and migration of smooth muscle cells.

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Section: мікрорнк-опосередкована активація трансляції що асоційована ...unclassified

Mechanisms of action of cytoplasmic microRNAs. Part 6. MicroRNA-mediated translation activation

Абатуров¹,

Babуch²

2023

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“…One of the two miRNA strands (the guide strand) is bound by the Argonaute (AGO) proteins loaded into the miRNA-induced silencing complex [39,40,43,44] and modulates cellular gene expression through the recognition of complementary regions within the mRNA target. Post-transcriptional inhibition of gene expression is the most reported and well-known mechanism of action of miRNAs, although under specific conditions they can also enhance gene expression [45][46][47][48]. Gene silencing is achieved through the recruitment of proteins that mediate mRNA deadenylation, decapping, and 5 -to-3 mRNA degradation [40,43].…”

Section: Micrornas (Mirnas): Role In Disease Pathogenesis and As Pote...mentioning

confidence: 99%

MicroRNAs in Juvenile Idiopathic Arthritis: State of the Art and Future Perspectives

Pelassa

Raggi

Rossi

et al. 2023

Biology

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Juvenile Idiopathic Arthritis (JIA) represents the most common chronic pediatric arthritis in Western countries and a leading cause of disability in children. Despite recent clinical achievements, patient management is still hindered by a lack of diagnostic/prognostic biomarkers and targeted treatment protocols. MicroRNAs (miRNAs) are short non-coding RNAs playing a key role in gene regulation, and their involvement in many pathologies has been widely reported in the literature. In recent decades, miRNA’s contribution to the regulation of the immune system and the pathogenesis of autoimmune diseases has been demonstrated. Furthermore, miRNAs isolated from patients’ biological samples are currently under investigation for their potential as novel biomarkers. This review aims to provide an overview of the state of the art on miRNA investigation in JIA. The literature addressing the expression of miRNAs in different types of biological samples isolated from JIA patients was reviewed, focusing in particular on their potential application as diagnostic/prognostic biomarkers. The role of miRNAs in the regulation of immune responses in affected joints will also be discussed along with their potential utility as markers of patients’ responses to therapeutic approaches. This information will be of value to investigators in the field of pediatric rheumatology, encouraging further research to increase our knowledge of miRNAs’ potential for future clinical applications in JIA.

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“…It should be noted that inclusion of TSs of multiple miRNAs in the same vector can allow detargeting of the same or multiple tissues in a cooperative manner [ 84 , 87 ]. Lastly, it has recently been observed that some miRNAs induce translation induction rather than inhibition in quiescence cells, which are cells reversibly arrested at phase G0 of the cell cycle [ 88 ]. miRNAs including let7 and miR369 induce upregulation of target mRNAs in the nucleus of G0 cells while repressing its expression in the cytoplasm of dividing ones [ 79 ].…”

Section: Factors Affecting the Design Of Mirna-regulated Gene Delimentioning

confidence: 99%

MicroRNA-Regulated Gene Delivery Systems for Research and Therapeutic Purposes

2018

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Targeted gene delivery relies on the ability to limit the expression of a transgene within a defined cell/tissue population. MicroRNAs represent a class of highly powerful and effective regulators of gene expression that act by binding to a specific sequence present in the corresponding messenger RNA. Involved in almost every aspect of cellular function, many miRNAs have been discovered with expression patterns specific to developmental stage, lineage, cell-type, or disease stage. Exploiting the binding sites of these miRNAs allows for construction of targeted gene delivery platforms with a diverse range of applications. Here, we summarize studies that have utilized miRNA-regulated systems to achieve targeted gene delivery for both research and therapeutic purposes. Additionally, we identify criteria that are important for the effectiveness of a particular miRNA for such applications and we also discuss factors that have to be taken into consideration when designing miRNA-regulated expression cassettes.

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Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells

Cited by 5 publications

References 63 publications

Mechanisms of action of cytoplasmic microRNAs. Part 6. MicroRNA-mediated translation activation

Mechanisms of action of cytoplasmic microRNAs. Part 6. MicroRNA-mediated translation activation

MicroRNAs in Juvenile Idiopathic Arthritis: State of the Art and Future Perspectives

MicroRNA-Regulated Gene Delivery Systems for Research and Therapeutic Purposes

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