Analysis of microbial volatile organic compounds produced by wood-decay fungi

Konuma, Rumi; Umezawa, Kiwamu; Mizukoshi, Atsushi; Kawarada, Kensuke; Yoshida, Makoto

doi:10.1007/s10529-015-1870-9

Cited by 22 publications

(7 citation statements)

References 18 publications

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“…Methyl furoates have been identified as MVOCs in fungal cultivations on Japanese beech wood (Konuma et al . 2015), but no clear relation with any of the compounds with either brown rot of white rot decay type could be recognized. In our study, NMDS orientation analysis moreover visualized the correspondence of methyl 3-furoate with white rot decay type, especially pointing the relation with fungal-produced manganese peroxidase activity, which is indicative of a strong oxidoreductive attack on lignin in wood (Hatakka and Hammel 2010; Floudas et al .…”

Section: Discussionmentioning

confidence: 93%

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Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns

Mali

Mäki

Hellén

et al. 2019

FEMS Microbiology Ecology

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Effect of three wood-decaying fungi on decomposition of spruce wood was studied in solid-state cultivation conditions for a period of three months. Two white rot species (Trichaptum abietinum and Phlebia radiata) were challenged by a brown rot species (Fomitopsis pinicola) in varying combinations. Wood decomposition patterns as determined by mass loss, carbon to nitrogen ratio, accumulation of dissolved sugars and release of volatile organic compounds (VOCs) were observed to depend on both fungal combinations and growth time. Similar dependence of fungal species combination, either white or brown rot dominated, was observed for secreted enzyme activities on spruce wood. Fenton chemistry suggesting reduction of Fe3+ to Fe2+ was detected in the presence of F. pinicola, even in co-cultures, together with substantial degradation of wood carbohydrates and accumulation of oxalic acid. Significant correlation was perceived with two enzyme activity patterns (oxidoreductases produced by white rot fungi; hydrolytic enzymes produced by the brown rot fungus) and wood degradation efficiency. Moreover, emission of four signature VOCs clearly grouped the fungal combinations. Our results indicate that fungal decay type, either brown or white rot, determines the loss of wood mass and decomposition of polysaccharides as well as the pattern of VOCs released upon fungal growth on spruce wood.

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Section: Discussionmentioning

confidence: 93%

“…Together with an array of terpenoids, methyl furoates (predicted as linoleic acid oxidation derived products) were identified in another study as MVOC products in beech-wood single-species cultures of a brown rot fungus ( Fomitopsis palustris ) and a white rot fungus ( Trametes versicolor ) (Konuma et al . 2015).…”

Section: Discussionmentioning

confidence: 99%

Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns

Mali

Mäki

Hellén

et al. 2019

FEMS Microbiology Ecology

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“…Recent studies have shown that fungal emission patterns can be species-specific, and chemotyping is possible for some species and functional groups (Müller et al 2013;Redeker et al 2018). Species-specific VOCs have already been defined for a few polypore species (Marshall 1970;Cowan 1973;McAfee & Taylor 1999;Rapior et al 2000;Rosecke et al 2000;Ziegenbein et al 2010;Konuma et al 2015). More generally, this confirms that direct mass spectrometry allows for a reliable species identification of wood decaying polypores, including a discrimination between F. fomentarius and Fomes inzengae (Pristas et al 2017).…”

Section: Volatile Organic Compoundsmentioning

confidence: 54%

“…At first, it is surprising that pure cultures produce a higher diversity and higher concentrations of VOCs than basidiomes. Wood-decaying fungi produce specific VOCs during wood degradation, and emission patterns depend on both the cultivation stage and the substrate (wood chips or potato dextrose agar), suggesting that wood degradation might activate synthetic pathways such as VOC production (Konuma et al 2015). Emission patterns of basidiomes could differ because hyphae are not physiologically active any more: no wood degradation occurs in basidiomes, and in those the hyphae have mainly structural (skeletal hyphae) and reproductive functions.…”

Section: Volatile Organic Compoundsmentioning

confidence: 99%

How to resolve cryptic species of polypores: an example in Fomes

et al. 2019

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Species that cannot be easily distinguished based on morphology, but which form distinct phylogenetic lineages based on molecular markers, are often referred to as cryptic species. They have been proposed in a number of fungal genera, including the basidiomycete genus Fomes. The main aim of this work was to test new methods for species delimitation in cryptic lineages of polypores, and to define useful characters for species identification. A detailed examination of a number of different Fomes strains that had been collected and isolated from different habitats in Italy and Austria confirmed the presence of distinct lineages in the Fomes fomentarius clade. Our zero hypothesis was that the Mediterranean strains growing on Quercus represent a species which can be delimited based on morphological and physiological characters when they are evaluated in statistically relevant numbers. This hypothesis was tested based on phylogenetic analysis of the rDNA ITS region, morphological characters of basidiomes and pure cultures, growth rates and optimum growth temperature experiments, mycelial confrontation tests, enzyme activity tests and volatile organic compound (VOC) production. The Mediterranean lineage can unambiguously be delimited from F. fomentarius. A syntype of an obscure and previously synonymized name, Polyporus inzengae, represents the Mediterranean lineage that we recognize as Fomes inzengae, a distinct species. The rDNA ITS region is useful for delimitation of Fomes species. Moreover, also a variety of morphological characters including hymenophore pore size, basidiospore size, and diameter of skeletal hyphae are useful delimiting characters. The ecology is also very important, because the plant host appears to be a central factor driving speciation. Physiological characters turned also out to be species-specific, e.g. daily mycelial growth rates or the temperature range of pure cultures. The production of VOCs can be considered as a very promising tool for fast and reliable species delimitation in the future.

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“…During this process, different types of fungi produce a unique grouping of microbial volatile organic compounds (MVOCs), which can also be used for species identification. This phenomenon indicates the possibility of using MVOCs as detection markers for wood decay fungus [1].…”

Section: Introductionmentioning

confidence: 99%

Identification of MVOCs Produced by Coniophora puteana and Poria placenta Growing on WPC Boards by Using Subtraction Mass Spectra

Kozicki

Wiejak

Piasecki

et al. 2019

IJERPH

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Volatile fungal metabolites are responsible for various odors and may contribute to a “sick building syndrome” (SBS) with a negative effect on the heath of building. The authors have attempted to fill the research gaps by analyzing microbial volatile organic compounds (MVOCs) originating from representatives of the Basidiomycetes class that grow on wood-polymer composite (WPC) boards. WPCs have been analyzed as a material exposed to biodeterioration. Indoor air quality (IAQ) is affected by the increased use of WPCs inside buildings, and is becoming a highly relevant research issue. The emission profiles of MVOCs at various stages of WPC decay have been demonstrated in detail for Coniophora puteana and Poria placenta, and used to set the European industrial standards for wood-decay fungi. Differences in the production of MVOCs among these species of fungi have been detected using the thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) method. This study identifies the production of alcohols, aldehydes, ketones, carboxylic acids and other compounds during one month of fungal growth. The identified level of metabolites indicates a relation between the level of air pollution and condition of the WPC material, which may become part of IAQ quantification in the future. The study points to the species-specific compounds for representatives of brown and white-rot fungi and the compounds responsible for their odor. In this study, 1-Octen-3-ol was indicated as a marker for their active growth, which is also associated with SBS. The proposed experimental set-up and data analysis are a simple and convenient way to obtain emission profiles of MVOCs from microbes growing on different materials.

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Analysis of microbial volatile organic compounds produced by wood-decay fungi

Abstract: As both fungi produced specific MVOCs in the course of wood degradation indicates the possibility of the application of MVOCs as detection markers for wood-decay fungus existing in woody materials.

Cited by 22 publications

References 18 publications

Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns

Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns

How to resolve cryptic species of polypores: an example in Fomes

Identification of MVOCs Produced by Coniophora puteana and Poria placenta Growing on WPC Boards by Using Subtraction Mass Spectra

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