Analysis of meticillin-susceptible and meticillin-resistant biofilm-forming Staphylococcus aureus from catheter infections isolated in a large Italian hospital

Petrelli, Dezemona; Repetto, Antonella; D'Ercole, Stefania; Rombini, Silvia; Ripa, Sandro; Prenna, Manuela; Vitali, Luca A.

doi:10.1099/jmm.0.47621-0

Cited by 39 publications

(37 citation statements)

References 48 publications

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“…In accordance with our findings, medical instruments were suggested as a common source of biofilm producing staphylococci (27,28). However, such high rates of biofilm producing isolates from wound and blood samples in our study are not uncommon, since several authors have shown high rates of biofilm formation among staphylococci obtained from these settings (29)(30)(31)).…”

Section: Discussionsupporting

confidence: 73%

“…In addition, these genes regulate the slime production (7,13). In our study, the presence of icaA gene in all tested isolates was associated with the presence of icaD gene and vice versa, which has also been mentioned by other authors (28,34). The frequency of icaA/D genes among our staphylococci isolates was relatively high; however, such remarkable rates of ica operon among staphylococci isolates are not unexpected, since the same finding has been implicated in several studies (19,34,35).…”

Section: Discussioncontrasting

confidence: 34%

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Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Zalipour

Ebrahim‐Saraie

Sarvari

et al. 2016

Jundishapur J Microbiol

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Background: Biofilm formation capacity is recognized as an important virulence factor in staphylococci that makes the organisms more resistant to antibiotics and host defenses. Objectives: This study aimed to determine the biofilm producing ability and presence of icaA/D genes in staphylococcal isolates obtained from different clinical specimens. Methods: This cross-sectional study was performed on a total of 151 staphylococcal isolates (79 Staphylococcus aureus and 72 S. epidermidis) obtained from different clinical specimens from February to August 2013 in Shiraz, Southwest of Iran. Slime production ability was evaluated using the both phenotypic (by cultivation of staphylococcal isolates on Congo red agar (CRA)) and genotypic (detection of the presence of icaA/D genes by PCR) methods. Results: Overall, of the 79 S. aureus isolates tested with CRA method, 64.7% of methicillin-resistant S. aureus (MRSA) isolates, and 46.7% of methicillin-sensitive S. aureus (MSSA) isolates were able to produce biofilm. The relative frequency of biofilm producing S. epidermidis isolates was 70.8% that was significantly higher than that of S. aureus isolates. The most common source of biofilm producing isolates in both S. aureus and S. epidermidis isolates was endotracheal tube (ETT) with 100% biofilm formation. Moreover, the presence of icaA/D genes was detected in 63.3% and 81.9% of S. aureus and S. epidermidis isolates, respectively. Conclusions: The remarkable rates of biofilm production ability among clinically isolated staphylococci emphasize the necessity of more effective infections control policies to prevent biofilm formation on medical devices and hospital environmental surfaces.

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Section: Discussionsupporting

confidence: 73%

Section: Discussioncontrasting

confidence: 34%

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Zalipour

Ebrahim‐Saraie

Sarvari

et al. 2016

Jundishapur J Microbiol

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“…A study was conducted in Egypt, which was about the effect of ciprofloxacin on bacterial adherence and biofilm formation, the results of this study showed that ciprofloxacin decreased biofilm synthesis by ≥60% (5). Particularly, ciprofloxacin is one of the most currently prescribed classes of antibiotics in both the hospital and community (19), so it can be a reason for increasing resistance to ciprofloxacin. Tetracyclines are protein synthesis inhibitors, broad-spectrum, bacteriostatic antibiotics that target the 30S ribosome and prevent binding of tRNA and effective against both Gram-positive and Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Biofilm Formation Among Methicillin Resistance Staphylococcus aureus Isolated From Nasal Carriers

Rezaei¹,

Moniri²,

Mousavi³

et al. 2013

Jundishapur J Microbiol

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Background: Methicillin -resistant staphylococcus aureus (MRSA) is associated with serious infections. Having the ability of biofilmformation decrease their susceptibility to antibiotics. Objectives: The aim of this study was to determine the prevalence of biofilm formation among MRSA isolated from nasal carriers in the Beheshti Teaching Hospital in Kashan, Iran. Materials and Methods: A cross-sectional study was conducted in 810 patients referred to emergency department in Beheshti Hospital in Kashan. Sterilized nasal swabs were used for collecting nasal bacteria. Nasal specimens were further recognized as S. aureus strains by standard biochemical tests, and MRSA isolates were detected by disk diffusion method. PCR assay was used for detecting mecA gene in MRSA isolates. The susceptibility of MRSA isolates to amikacin, clindamycin, gentamicin, ciprofloxacin, SXT, erythromycin, tetracycline were determined by using disk diffusion method according to recommendation of CLSI. Biofilm formation ability of MRSA isolates were examined by crystal violet microtitre plate assay and Congo red agar (CRA). Results: Two hundred and ninety six (36.5%) out of 810 isolates were S. aureus. Twenty six (8.8%) of all S. aureus isolates were recognized as MRSA. All the MRSA isolates have the ability of biofilm formation which 15.4%, 19.2% and 65.4% of them were strong, medium and weak biofilm producer respectively. The resistance rate of strong biofilm producer were; erythromycin (100%), clindamycin (75%), ciprofloxacin (75%), SXT (75%), gentamycin (50%), tetracycline (0%), amikacin (0%). Conclusions: High rate of MRSA nasal carrier and having the ability of biofilm formation which decrease their susceptibility to antibiotics, is an alarming for public health. Statistically significant correlation between susceptibility to tetracycline and MRSA carrier was observed.

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“…In view of the appreciable activity against S. aureus, an additional series of S. aureus clinical isolates from catheter-associated infections was selected to confirm the observation and to prove the extendibility of the antimicrobial activity of the essential oil beyond the reference strain ATCC 29213. Four isolates were selected based on their proven resistance towards oxacillin, the congener of methicillin [25]. Hence, the four isolates were MRSA (methicillin-resistant Staphylococcus aureus).…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Biological Activities of the Essential Oil from Erigeron floribundus

et al. 2016

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Erigeron floribundus (Asteraceae) is an herbaceous plant widely used in Cameroonian traditional medicine to treat various diseases of microbial and non-microbial origin. In the present study, we evaluated the in vitro biological activities displayed by the essential oil obtained from the aerial parts of E. floribundus, namely the antioxidant, antimicrobial and antiproliferative activities. Moreover, we investigated the inhibitory effects of E. floribundus essential oil on nicotinate mononucleotide adenylyltransferase (NadD), a promising new target for developing novel antibiotics, and Trypanosoma brucei, the protozoan parasite responsible for Human African trypanosomiasis. The essential oil composition was dominated by spathulenol (12.2%), caryophyllene oxide (12.4%) and limonene (8.8%). The E. floribundus oil showed a good activity against Staphylococcus aureus (inhibition zone diameter, IZD of 14 mm, minimum inhibitory concentration, MIC of 512 µg/mL). Interestingly, it inhibited the NadD enzyme from S. aureus (IC 50 of 98 µg/mL), with no effects on mammalian orthologue enzymes. In addition, T. brucei proliferation was inhibited with IC 50 values of 33.5 µg/mL with the essential oil and 5.6 µg/mL with the active component limonene. The essential oil exhibited strong cytotoxicity on HCT 116 colon carcinoma cells with an IC 50 value of 14.89 µg/mL, and remarkable ferric reducing antioxidant power (tocopherol-equivalent antioxidant capacity, TEAC = 411.9 µmol·TE/g).

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Analysis of meticillin-susceptible and meticillin-resistant biofilm-forming Staphylococcus aureus from catheter infections isolated in a large Italian hospital

Cited by 39 publications

References 48 publications

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Prevalence of Biofilm Formation Among Methicillin Resistance Staphylococcus aureus Isolated From Nasal Carriers

Biological Activities of the Essential Oil from Erigeron floribundus

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