Analysis of metalaxyl racemate using high performance liquid chromatography coupled with four kinds of detectors

Chen, Tao; Fan, Jun; Gao, Ruiqi; Wang, Tai; Yu, Ying; Zhang, Weiguang

doi:10.1016/j.chroma.2016.07.007

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…The average and the range of the OR detector were set at off and 4.0 mdeg, respectively. Separation parameters such as retention factor ( k ), selectivity ( α ), and resolution ( R s ) were determined according to standard formulas on the basis of USP standards …”

Section: Methodsmentioning

confidence: 99%

“…In addition, the OR and refractive index (RI) detectors became the preferred choices in HPLC studies for the analytes with nonultraviolet active groups. Especially the OR detector as a highly selective detector is totally free from nonoptically active compounds . Goodall's group investigated the semipreparation of warfarin enantiomers through HPLC with an improved OR detector .…”

Section: Introductionmentioning

confidence: 99%

“…Especially the OR detector as a highly selective detector is totally free from nonoptically active compounds. [20][21][22] Goodall's group investigated the semipreparation of warfarin enantiomers through HPLC with an improved OR detector. 21 Our group reported a comparative evaluation of HPLC coupled with UV, OR, RI, and evaporative light scattering detectors for qualitative and quantitative analyses of metalaxyl racemate, and the effects of separation conditions on the peak area ratio between two enantiomers, including sample concentration, column temperature, mobile phase composition, and flow rate, have been investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

“…21 Our group reported a comparative evaluation of HPLC coupled with UV, OR, RI, and evaporative light scattering detectors for qualitative and quantitative analyses of metalaxyl racemate, and the effects of separation conditions on the peak area ratio between two enantiomers, including sample concentration, column temperature, mobile phase composition, and flow rate, have been investigated in detail. 22 In this work, isoborneol enantiomers were successfully resolved on the cellulose derivative-coated CSP by HPLC with a hexane-ethanol mixture. Taking its resolution into consideration, the effects of types of CSPs, alcoholic modifiers, and column temperature are discussed in detail.…”

Section: Introductionmentioning

confidence: 99%

“…Goodall's group investigated the semipreparation of warfarin enantiomers through HPLC with an improved OR detector . Our group reported a comparative evaluation of HPLC coupled with UV, OR, RI, and evaporative light scattering detectors for qualitative and quantitative analyses of metalaxyl racemate, and the effects of separation conditions on the peak area ratio between two enantiomers, including sample concentration, column temperature, mobile phase composition, and flow rate, have been investigated in detail . In this work, isoborneol enantiomers were successfully resolved on the cellulose derivative‐coated CSP by HPLC with a hexane‐ethanol mixture.…”

Section: Introductionmentioning

confidence: 99%

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Reliable HPLC separation, vibrational circular dichroism spectra, and absolute configurations of isoborneol enantiomers

et al. 2017

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Resolution of chiral compounds has played an important role in the pharmaceutical field, involving detailed studies of pharmacokinetics, physiological, toxicological, and metabolic activities of enantiomers. Herein, a reliable method by high-performance liquid chromatography (HPLC) coupled with an optical rotation detector was developed to separate isoborneol enantiomers. A cellulose tris(3, 5-dimethylphenylcarbamate)-coated chiral stationary phase showed the best separation performance for isoborneol enantiomers in the normal phase among four polysaccharide chiral packings. The effects of alcoholic modifiers and column temperature were studied in detail. Resolution of the isoborneol racemate displayed a downward trend along with an increase in the content of ethanol and column temperature, indicating that less ethanol in the mobile phase and lower temperature were favorable to this process. Moreover, two isoborneol enantiomers were obtained via a semipreparative chiral HPLC technique under optimum conditions, and further characterized by analytical HPLC, and experimental and calculated vibrational circular dichroism (VCD) spectroscopy, respectively. The solution VCD spectrum of the first-eluted component was consistent with the Density Functional Theory (DFT) calculated pattern based on the SSS configuration, indicating that this enantiomer should be (1S, 2S, 4S)-(+)-isoborneol. Briefly, these results have provided reliable information to establish a method for analysis, preparative separation, and absolute configuration of chiral compounds without typical chromophoric groups.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%