Analysis of Metabolite Profiling in Human Endothelial Cells after Plasma Jet Treatment

Abstract

Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in wound healing, dermatological treatment, hemostasis, and cancer treatment. In most cases, CAP treatment will interact with innumerable blood capillaries. Therefore, it is important and necessary to understand the effects of CAP treatment on endothelial cell metabolism. In this study, the metabolite profiling of plasma treatment on endothelial cells was measured by gas chromatography tandem time-of-flight mass spectrometry (GC-TOF-MS). We found that 695 signals (metabolites) were detected by GC-TOF-MS and then evaluated using orthogonal projections to latent structures discriminant analysis (OPLS-DA). All the differential metabolites were listed, and proline and xanthosine were the two of the most downregulated metabolites by plasma treatment. By comprehensive metabolic pathway analysis with the KEGG pathway, we showed that alanine, aspartate, glutamate, and purine metabolism pathways were the most significantly suppressed after gas plasma treatment in human endothelial cells. Our finding gives an overall picture of the metabolic pathways affected by plasma treatment in endothelial cells.

Intracellular Responses Triggered by Cold Atmospheric Plasma and Plasma-Activated Media in Cancer Cells

“…Glutaminase activity decreases after CAP treatment, leading to intracellular glutamine accumulation and leukaemia cells death [ 75 ]. In vivo CAP treatment of endothelial cells results in downregulated xantosine and proline metabolites, though KEGG pathway analysis revealed alanine, aspartate, glutamate, and purine metabolism pathways to be most suppressed [ 76 ]. Yet, He-CAP treatment of myeloma cells revealed the beta-alanine metabolism pathway to be most changed, followed by propanoate and linoleic acid metabolism [ 77 ].…”