Analysis of Metabolic Network of Synthetic Escherichia coli Producing Linalool Using Constraint-based Modeling

Tawornsamretkit, Iyarest; Thanasomboon, Rattana; Thaiprasit, Jittrawan; Waraho, Dujduan; Cheevadhanarak, Supapon; Meechai, Asawin

doi:10.1016/j.procs.2012.09.004

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Here, metabolite uptake rates were obtained from previous modeling studies that characterized the growth of E. coli in LB medium (Tawornsamretkit et al, 2012). To simulate uracil availability, the uracil uptake flux in the model was set to 9 mmol/g dW/hr, which corresponds to the maximum uracil production that can be achieved by the metabolic network from LB medium, and therefore represents a saturation condition that requires no de novo biosynthesis of uracil for any objective function.…”

Section: Star * Methodsmentioning

confidence: 99%

Bacterial Metabolism Affects the C. elegans Response to Cancer Chemotherapeutics

García-González

Ritter

Shrestha

et al. 2017

Cell

205

161

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SUMMARY The human microbiota greatly affects physiology and disease. However, the contribution of bacteria to the response to chemotherapeutic drugs remains poorly understood. Caenorhabditis elegans and its bacterial diet provide a powerful system to study host-bacteria interactions. Here, we use this system to study how bacteria affect the C. elegans response to chemotherapeutics. We find that different bacterial species can increase the response to one drug yet decrease the effect of another. We perform genetic screens in two bacterial species using three chemotherapeutic drugs, 5-fluorouracil (5-FU), 5-fluoro-2′-deoxyuridine (FUDR) and camptothecin (CPT). We find numerous bacterial nucleotide metabolism genes that affect drug efficacy in C. elegans. Surprisingly, we find that 5-FU and FUDR act through bacterial ribonucleotide metabolism to elicit their cytotoxic effects in C. elegans, rather than by thymineless death or DNA damage. Our study provides a blueprint for characterizing the role of bacteria in the host response to chemotherapeutics.

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Section: Star * Methodsmentioning

confidence: 99%

Bacterial Metabolism Affects the C. elegans Response to Cancer Chemotherapeutics

García-González

Ritter

Shrestha

et al. 2017

Cell

205

161

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“…In addition, original maintenance energy requirements were preserved (Orth et al, 2011), while no constraints were imposed to force growth (biomass formation) (Table S6). To define the main nutritional input, exchange reactions were constrained according to (Tawornsamretkit et al, 2012) assuming an LB growth medium. However, the lower boundary of all exchange and sink reactions that are not part of these medium constraints was set at -0.1 mmol/gDW/h to allow other nutrients to be used by the model so that all reactions could carry flux during FBA.…”

Section: Metabolic Network Analysesmentioning

confidence: 99%

A Delicate Balance between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development

Zhang¹,

Li²,

Olmedo³

et al. 2019

Cell Host & Microbe

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Iron is an essential micronutrient for all forms of life; low levels of iron cause human disease, while too much iron is toxic. Low iron levels induce reactive oxygen species (ROS) by disruption of the heme and iron-sulfur cluster-dependent electron transport chain (ETC). To identify bacterial metabolites that affect development, we screened the Keio Escherichia coli collection and uncovered 244 gene deletion mutants that slow Caenorhabditis elegans development. Several of these genes encode members of the ETC cytochrome bo oxidase complex, as well as iron importers. Surprisingly, either iron or anti-oxidant supplementation reversed the developmental delay. This suggests that low bacterial iron results in high bacterial ROS and vice versa, which causes oxidative stress in C. elegans that subsequently impairs mitochondrial function and delays development. Our data indicate that the bacterial diets of C. elegans provide precisely tailored amounts of iron to support proper development.

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Analysis of Metabolic Network of Synthetic Escherichia coli Producing Linalool Using Constraint-based Modeling

Cited by 2 publications

References 15 publications

Bacterial Metabolism Affects the C. elegans Response to Cancer Chemotherapeutics

Bacterial Metabolism Affects the C. elegans Response to Cancer Chemotherapeutics

A Delicate Balance between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development

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