Analysis of Medicinal Properties of the Co-1 Cultivar of Fenugreek Leaf Oil by Gc-MS Metabolite Profiling

Abstract

The leguminous family of plants includes fenugreek (Trigonella-Foenum Graecum), one of the oldest and most promising therapeutic herbs. The seeds are mostly used as spices to flavour practically all recipes, while the fresh, delicate leaves and stem are eaten as curry vegetables. The Co.1 variety from the TG-2356 strain was done for North India. Dual purpose variety evolved at TNAU, yield of 680 kg/ha on average, lasting 80 to 85 days. The key characteristics are early maturing varieties with fast growth and resistance to root rot disease. Fenugreek seeds have been the subject of in-depth research for the treatment of diabetes, cancer, and inflammation but not about leaves. In this investigation, fenugreek leaf oil was extracted and subjected to gas chromatography coupled to mass spectrometry analysis to determine its chemical compositions (GC-MS). Investigating the chemical make-up of physiologically active chemicals from fenugreek leaf oil is the goal of this study. Through GC-MS analysis, a total of 40 chemical components, accounting for 99% of the oil, were found and identified in fenugreek seed oil.Aziridine,1,2,3-trimethyl-, trans-(7.11% and 16.47%), Cyclohexane,1,1'-(1,2-dimethyl-1,2-ethanediyl)bis-,(R*,R*)-(n)-(2.85%), Sucrose (2.68%), 3-O-Methyl-d-glucose (7.36%), 9, 12-Octadecadienoic acid (Z,Z)-(1.47%), Oleic Acid (1.49%), Heptadecane, 9-hexyl-(1.23%), Heptacosane (4.38%) were the main components of the extracted oil. Due to their inherent pharmaceutical qualities, were shown to be extremely helpful in decreasing free radicals. Overall, fenugreek leaf oil may be useful against various illnesses due to its anticancer, antioxidant, antiinflammatory, antitumor, antibacterial, diuretic, and antifungal characteristics, according to the results of this study. As a result, this study implies that fenugreek leaf oil could have medical use.