Analysis of maternal and neonatal factors that influence the nucleated and CD34+ cell yield for cord blood banking

Nakagawa, Ryuji; Watanabe, Tsutomu; Kawano, Yoshifumi; Kanai, Sachiyo; Suzuya, Hiroko; Kaneko, Michiya; Watanabe, Hiroyoshi; Okamoto, Yasuhiro; Kuroda, Yasuhiro; Nakayama, T

doi:10.1111/j.1537-2995.2004.00645.x

Cited by 104 publications

(164 citation statements)

References 24 publications

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“…These results are consistent with several previous studies showing that maternal age does not affect either nucleated cells or CD34 + cells (Donaldson et al 1999;Nakagawa et al 2004;George et al 2006;Mancinelli et al 2006). However, focusing on the 1-gravidae alone, the maternal age of 1-gravidae have a slightly positive correlation with the total LD cells (Fig.…”

Section: Discussionsupporting

confidence: 83%

“…Some previous studies described the context of CB banking regarding the cost of CB cell processing and freezing in storage (Sirchia et al 1998(Sirchia et al , 1999Ballen et al 2001;Nakagawa et al 2004). Other previous studies also demonstrated the correlations between the number of nucleated/CD34 + cells and maternal/neonatal factors (Donaldson et al 1999;Yamada et al 2000;Li et al 2001;Askari et al 2005;George et al 2006;Mancinelli et al 2006).…”

Section: Cb Units Collected For Subjectsmentioning

confidence: 91%

“…In this process of separations, the red blood cells and granulocytes are fully removed during the treatment of Ficoll-Paque centrifugation and the purification of CD34 + cells. According to other previous reports (Ballen et al 2001;Mohyeddin Bonab et al 2004;Nakagawa et al 2004), hydroxyethyl starch is used for the separation of nucleated cells and the contents of CD34 + cells are analyzed by flow cytometry. Therefore it could be difficult to compare the absolute numbers of cells in our results with others because the total nucleated cells in other previous reports seem to contain more granulocytes.…”

Section: Discussionmentioning

confidence: 99%

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Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34+ Cells from Placental/Umbilical Cord Blood

Omori

Takahashi

Hazawa

et al. 2008

Tohoku J. Exp. Med.

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Placental/umbilical cord blood (CB) contains nucleated cells and hematopoietic stem/ progenitor cells (CD34 + cells). However it is difficult to predict the number of nucleated/ CD34 + cells in each CB before cell processing. Despite many previous studies from institutes affiliated with CB banks in metropolitan areas, little information is available regarding the characteristics of CB units from other medical facilities. The purpose of the present study was to analyze the maternal/neonatal factors on the yield of cells in CB units. A total of 176 CB units were obtained from single-birth and normal vaginal deliveries. Mononuclear low-density (LD) cells were separated using Ficoll-Paque within 24 hrs after CB collection and then processed for the purification of CD34 + cells. A multiple linear regression analysis was performed to assess the correlations between the yield of cells and maternal/neonatal factors including maternal age, gravid status, duration of labor, gestational age, neonatal height and weight, cord length, and meconium in the amniotic fluid. The total LD cells per CB unit had a weak positive correlation with the maternal age of primigravidae. The total LD cells per CB unit from the primigravidae aged 25 were significantly higher than those from the primigravidae aged 24. The total CD34 + cells per CB unit from the 1-gravidae were significantly higher than those from the 2-gravidae and 3-gravidae, respectively among all donors. These results indicate that the CB units from the primigravidae aged 25 are more likely to contain higher yield of LD/CD34 + cells. cord blood; mononuclear cells; CD34 + cells; maternal/neonatal factors.Tohoku

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Section: Discussionsupporting

confidence: 83%

Section: Cb Units Collected For Subjectsmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34+ Cells from Placental/Umbilical Cord Blood

Omori

Takahashi

Hazawa

et al. 2008

Tohoku J. Exp. Med.

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“…8,9 Studies performed on healthy pregnancies have shown that gestational and obstetric factors, including maternal age, parity, gestational age, length of labor, stress during labor, route of delivery, technique of UCB collection and positioning of the newborn after delivery can influence the numbers of HSC in UCB obtained after delivery. [10][11][12][13][14] A younger maternal age, shorter gestational age and shorter time from collection to processing were associated with higher CD34 + HSC counts. 14 Neonatal characteristics, including gender and weight of the newborn, may also influence the numbers of HSC in UCB.…”

Section: Introductionmentioning

confidence: 99%

“…[10][11][12][13][14] A younger maternal age, shorter gestational age and shorter time from collection to processing were associated with higher CD34 + HSC counts. 14 Neonatal characteristics, including gender and weight of the newborn, may also influence the numbers of HSC in UCB. Bigger babies had higher NCC, more CD34 + cells and colony-forming units granulocyte-macrophage (CFU-GM).…”

Section: Introductionmentioning

confidence: 99%

Effects of preeclampsia on the yield of hematopoietic stem cells obtained from umbilical cord blood at delivery

Fadilah

Nasaruddin²,

Idris

et al. 2012

J of Obstet and Gynaecol

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Aim: To compare the numbers of cord blood CD34+ hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non-PE) subjects and to determine the factors that may influence this observation. Methods: Umbilical cord blood was collected from 28 PE and 19 non-PE subjects. Nucleated and CD34+ cell counts were derived using the Trucount tube-based stem cell enumeration kit on BD FACSCalibur. Results: The cord blood volume, nucleated and CD34+ cell counts were significantly reduced in PE subjects compared to non-PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34 + cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34 + cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34 + cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects. Conclusion: Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.

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Cellular Engineering for the Production of New Blood Components

2016

Transfusion Medicine

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Analysis of maternal and neonatal factors that influence the nucleated and CD34+ cell yield for cord blood banking

Abstract: Maternal and neonatal factors appeared to affect CB cell yields. These findings might be useful for efficiently collecting more qualified CB units.

Cited by 104 publications

References 24 publications

Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34+ Cells from Placental/Umbilical Cord Blood

Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34+ Cells from Placental/Umbilical Cord Blood

Effects of preeclampsia on the yield of hematopoietic stem cells obtained from umbilical cord blood at delivery

Cellular Engineering for the Production of New Blood Components

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